ABSTRACT
Soluble antigen (SA) from chlamydial elementary bodies (EBs) was extracted with N-lauroylsarcosine. The extracted SA composed of lipopolysaccharide (LPS) and proteins was compared with EBs using an enzyme-linked immunosorbent assay (ELISA). Patient sera from natural chlamydial infections exhibited ELISA mean absorbance (A(492) and A(405/650)) values 2-5 times higher with SA than with EBs, resulting in a better discrimination between positive and negative human sera.
Subject(s)
Antibodies, Bacterial/blood , Antigens, Bacterial/chemistry , Chlamydia Infections/diagnosis , Chlamydia trachomatis/immunology , Enzyme-Linked Immunosorbent Assay/methods , Lipopolysaccharides/chemistry , Sarcosine/analogs & derivatives , Adult , Blotting, Western , Chlamydia trachomatis/chemistry , Detergents/chemistry , Electrophoresis, Polyacrylamide Gel , Female , Humans , Male , Middle Aged , Sarcosine/chemistryABSTRACT
A cross-reactivity among some strains of Coxiella burnetii and chlamydiae with immune rabbit and mouse sera in ELISA and immunoblot analysis was observed. In the latter, the cross-reactivity disappeared after a treatment of C. burnetii or C. psittaci with proteinase K, which indicates that only proteins were involved. The observed cross-reactivity was not influenced by host chick embryo yolk sac proteins. After adsorption of immune rabbit sera with homologous corpuscular antigens the cross-reactivity disappeared. The possibility of influence of such cross-reactivity on serological diagnosis of C. burnetii or chlamydiae infections is discussed.
