[Comparative characteristics of the chemical composition of vitreal contents of cadaver eyes and eyes with terminal refractory glaucoma]. / Sravnitel'naia kharakteristika khimicheskogo sostava vitreal'nogo soderzhimogo kadavernykh glaz i glaz s refrakternoi terminal'noi glaukomoi.

PURPOSE: To compare the chemical elemental composition of vitreous cavity content taken from cadaveric eyes compared to samples taken from the eyes with terminal stage refractory glaucoma with decompensated intraocular pressure (IOP). MATERIAL AND METHODS: The vitreous contents of the eyes from 2 groups were studied. The 1st group included 15 cadaveric eyes; the 2nd group included 15 eyes with refractory glaucoma in the terminal stage of the disease with decompensated IOP in patients with hypertension pain. The vitreal content samples were taken in the course of antiglaucoma surgery aimed at preserving the eye as an organ and involving employment of drainage in the vitreous cavity. The study of virtual contents was carried out on energy dispersive spectrometer Oxford X-Max 50 integrated into scanning electron microscope Zeiss EVO LS10. RESULTS: Increased concentrations of Kalium and Phosphorus were detected in the vitreous content of cadaveric eyes compared with the vitreal content from the eyes with terminal glaucoma with decompensated IOP taken in vivo (K - 0.172/0.093; P - 0.045/0.025 mmol/L). In the vitreous cavity in the eyes with end-stage glaucoma with decompensated IOP, the concentration of Nitrogen was higher in comparison with human cadaver eyes (2.030/1.424 mmol/L). CONCLUSION: The increased concentrations of Kalium and Phosphorus in the vitreous content of cadaveric eyes is associated with postmortem autolytic processes and with the release of intracellular content in the destruction of cell membranes. The increased Nitrogen concentration in the vitreal contents of the eyes with terminal stage glaucoma with decompensated IOP may be associated with the presence of osmotically active nitrogen-containing compounds in the eyes with increased IOP.

[Influence of chemical compositions of anterior chamber aqueous humour and blood serum on the secretion of intraocular fluid]. / Vliianie khimicheskogo sostava vlagi perednei kamery glaza i syvorotki krovi na sekretsiiu vnutriglaznoi zhidkosti.

PURPOSE: to identify the standard chemical composition of anterior chamber aqueous humour (AH) using energy dispersive spectrometer (EDS) integrated in scanning electron microscope (SEM); to study the influence of concentration gradient of chemical elements between aqueous humor and blood serum (BS) on the secretion of intraocular fluid (IOF) at normal and increased intraocular pressure (IOP). MATERIAL AND METHODS: The study involved two groups of patients consisting of 33 people (33 eyes) each. The 1st group included patients with cataract and normal IOP (control), the 2nd group included patients with POAG and decompensated IOP. The samples (AH and BS) were taken during surgeries and studied using Oxford X-Max50 EDS integrated in EVO LS10 SEM. RESULTS: The concentration ratios (mmol/l) of AH/BS for Na at normal IOP was 1.472/1.278, for K - 0.106/0.035. In cases with POAG with decompensated IOP, the concentration ratio of Na was 1.424/1.248, K - 0.114/0.036. The concentration ratio of N between AH in cases with decompensated IOP and AH of normotonic eyes was 1.151/0.960, for S - 0.020/0.012. CONCLUSION: Consistent excess of Na and K concentrations in AH compared to BS indicates active participation of these osmotically active elements in the normal process of intraocular fluid secretion. The absence of significant differences in the ratios of Na and K in AH and BS at normal and decompensated IOP indicates low significance of these elements for pathological hypersecretion of intraocular fluid. Increased concentration of N in AH eyes with decompensated IOP compared with AH at normal IOP indicates possible involvement of nitrogen-containing osmotically active substances in the abnormal hypersecretion of intraocular fluid.