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Jpn J Cancer Res ; 89(5): 525-32, 1998 May.
Article in English | MEDLINE | ID: mdl-9685856

ABSTRACT

A human tumor cell line designated RMS-GR was established from an embryonal rhabdomyosarcoma. The monolayer cells were polygonal, round or spindle-shaped. The RMS-GR cell line became stable with a doubling time of 42 h. Tumorigenicity of the cells was confirmed by heterotransplantion into nude mice. Electron microscopic images showed typical cytoplasmic inclusion of aggregated intermediate filaments and myofibril-like thin filaments. The expression of desmin, vimentin, actin and human myoglobin was recognized by cytofluorometric analyses, and a large fraction of CK-MM and small fractions of CK-BB and MCK-1 isoenzymes were found. Chromosomal analysis showed that the modal chromosome number was consistently near triploid with structural abnormalities mostly involving chromosomes 1, 3 and 8, and additional unidentified markers. No alteration of chromosome 2 was observed. The RMS-GR cell line may provide a system to identify genes which are involved in the pathogenic mechanism of rhabdomyosarcomas, and to investigate the modulation of myogenic differentiation.


Subject(s)
Rhabdomyosarcoma, Embryonal/pathology , Tumor Cells, Cultured , Actins/analysis , Animals , Cell Division , Chromosome Aberrations , Creatine Kinase/analysis , Humans , Isoenzymes , Keratins/analysis , Mice , Mice, Nude , Myoglobin/analysis , Neoplasm Transplantation , Rhabdomyosarcoma, Embryonal/chemistry , Rhabdomyosarcoma, Embryonal/genetics , Transplantation, Heterologous , Vimentin/analysis
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