ABSTRACT
The complete nucleotide sequence of the D10H fragment (10850 bp) was determined. The D10H fragment is located on the right arm of chromosome III near the centromere and contains the SUF2 gene. Six open reading frames (ORFs) larger than 300 bp were found. One of them is the CIT2 gene encoding the cytoplasmic citrate synthase. The others are new putative genes and show no significant similarity with any known gene. In addition two tRNA genes (Asn and Pro) and a solo delta element were identified. Two ORFs were disrupted; no peculiar phenotype was observed.
Subject(s)
Chromosome Mapping , Chromosomes, Fungal , DNA, Fungal/genetics , Saccharomyces cerevisiae/genetics , Base Sequence , Centromere , Citrate (si)-Synthase/genetics , Cloning, Molecular , Molecular Sequence Data , RNA, Transfer, Asn/genetics , RNA, Transfer, Pro/genetics , Reading Frames , Regulatory Sequences, Nucleic Acid/genetics , Sequence Homology, Nucleic AcidABSTRACT
Representative strains of the known groups and serogroups of spiroplasmas have been compared on the basis of the results of one- and two-dimensional protein analysis on polyacrylamide gels. Each of the four subgroups of the Spiroplasma citri complex (S. citri and honeybee, corn stunt, and 277F spiroplasmas) has characteristic protein profiles and maps. Spiroplasma G1 isolated from flowers has a protein profile closely related to that of the honeybee spiroplasmas (KC3, BC3, AS576, B1707, B88, and B63). The flower spiroplasmas fall into two groups on the basis of the mole percentage (mol%) of guanine plus cytosine (G + C) of their deoxyribonucleic acid (DNA) 26 and 30 mol %, respectively) and their serologic properties. The three flower spiroplasmas (OBMG, BNR1, and 23-6) with 26 mol % of G + C have the same basic protein pattern. Although some of the flower spiroplasmas with 30 mol % of G + C have closely related protein patterns, others have more distant profiles. Spiroplasmas B13 and L89, isolated from honeybees and froghoppers, respectively, had DNA with 30 mol % of G + C and could be included with other flower spiroplasmas with similar DNA base composition on the basis of their protein profiles and maps. Individual proteins separated by two-dimensional analysis on polyacrylamide gels have been used for the production of monospecific immunoglobulins, which are useful in taxonomic studies.
