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1.
Mol Cell Endocrinol ; 377(1-2): 65-74, 2013 Sep 05.
Article in English | MEDLINE | ID: mdl-23835445

ABSTRACT

Glucose regulates energy homeostasis and reproductive function within the hypothalamus. The underlying mechanisms responsible for glucose regulation of GnRH gene transcription were investigated using a novel murine immortalized, adult-derived hypothalamic cell line, mHypoA-GnRH/GFP. Analysis of GnRH mRNA synthesis and secretion following agonist treatment demonstrated that the mHypoA-GnRH/GFP cell line is a representative model of in vivo GnRH neurons. c-fos mRNA levels, following glucose exposure, indicated that these neurons were responsive to low (0.5mM) and high (5mM) glucose, and high glucose stimulated GnRH mRNA transcription in a metabolism-dependent manner. Glucose inhibited AMPK activity, and was linked to the downstream stimulation of GnRH mRNA levels. The effect was confirmed with an AMPK antagonist, Compound C. Collectively, these findings demonstrate that glucose can directly regulate GnRH transcription, while implicating the AMPK pathway as an essential mediator of nutritional signaling in a novel GnRH neuronal cell model.


Subject(s)
AMP-Activated Protein Kinases/metabolism , Aging/metabolism , Glucose/pharmacology , Gonadotropin-Releasing Hormone/metabolism , Green Fluorescent Proteins/metabolism , AMP-Activated Protein Kinases/antagonists & inhibitors , Animals , Cell Line , Colforsin/pharmacology , Cyclic AMP-Dependent Protein Kinases/metabolism , Gene Expression Regulation/drug effects , Gonadotropin-Releasing Hormone/genetics , Mice , Models, Biological , Neurons , Nitric Oxide/metabolism , Peptides/metabolism , Phosphorylation/drug effects , Protein Kinase C/metabolism , Protein Kinase Inhibitors/pharmacology , Proto-Oncogene Proteins c-fos/genetics , Proto-Oncogene Proteins c-fos/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Second Messenger Systems , Signal Transduction/drug effects , Tetradecanoylphorbol Acetate/pharmacology
2.
Mol Endocrinol ; 27(6): 990-1003, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23579487

ABSTRACT

Central resistance to the actions of insulin and leptin is associated with the onset of obesity and type 2 diabetes mellitus, whereas leptin and insulin signaling is essential for both glucose and energy homeostasis. Although it is known that leptin resistance can lead to attenuated insulin signaling, whether insulin resistance can lead to or exacerbate leptin resistance is unknown. To investigate the molecular events underlying crosstalk between these signaling pathways, immortalized hypothalamic neuronal models, rHypoE-19 and mHypoA-2/10, were used. Prolonged insulin exposure was used to induce cellular insulin resistance, and thereafter leptin-mediated regulation of signal transduction and gene expression was assessed. Leptin directly repressed agouti-related peptide mRNA levels but induced urocortin-2, insulin receptor substrate (IRS)-1, IRS2, and IR transcription, through leptin-mediated phosphatidylinositol 3-kinase/Akt activation. Neuronal insulin resistance, as assessed by attenuated Akt phosphorylation, blocked leptin-mediated signal transduction and agouti-related peptide, urocortin-2, IRS1, IRS2, and insulin receptor synthesis. Insulin resistance caused a substantial decrease in insulin receptor protein levels, forkhead box protein 1 phosphorylation, and an increase in suppressor of cytokine signaling 3 protein levels. Cellular insulin resistance may cause or exacerbate neuronal leptin resistance and, by extension, obesity. It is essential to unravel the effects of neuronal insulin resistance given that both peripheral, as well as the less widely studied central insulin resistance, may contribute to the development of metabolic, reproductive, and cardiovascular disorders. This study provides improved understanding of the complex cellular crosstalk between insulin-leptin signal transduction that is disrupted during neuronal insulin resistance.


Subject(s)
Insulin Resistance , Leptin/physiology , Signal Transduction , Transcription, Genetic , Androstadienes/pharmacology , Animals , Cells, Cultured , Chromones/pharmacology , Extracellular Signal-Regulated MAP Kinases/metabolism , Gene Expression Regulation , Hypothalamus/cytology , Insulin/physiology , Janus Kinase 2/metabolism , Mice , Morpholines/pharmacology , Neurons/metabolism , Phosphatidylinositol 3-Kinases/metabolism , Phosphoinositide-3 Kinase Inhibitors , Phosphorylation , Protein Processing, Post-Translational , Proto-Oncogene Proteins c-akt/antagonists & inhibitors , Proto-Oncogene Proteins c-akt/metabolism , Rats , STAT3 Transcription Factor/metabolism , Wortmannin
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