Thyroid metastasis of vaginal leiomyosarcoma: a case report and review of the literature.

Metastasis of vaginal leiomyosarcoma to the thyroid gland is an extremely rare event. Here, we report a case of thyroid metastasis of vaginal leiomyosarcoma. A 65-year-old female patient presented with a multinodular toxic goiter. 5 years earlier the patient has undergone a radical vaginal resection followed by chemotherapy due to high-grade vaginal leiomyosarcoma. Fine-needle aspiration cytology of the right thyroid lobe was suggestive of the benign thyroid lesion. As the diagnosis was not clear, following a multidisciplinary team discussion the decision was made to proceed with a total thyroidectomy. Pathologic assessment of the tumor confirmed thyroid metastasis of vaginal leiomyosarcoma.

Periodontitis agresiva infantil en un niño de 4 años / Aggressive infantile periodontitis in a 4-year-old boy

Se define a la periodontitis agresiva como una forma agresiva y rápidamente destructiva de enfermedad, caracterizada por la pérdida severa del ligamento periodontal y hueso alveolar. Si bien la mayor prevalencia se ubica en la población adulta, se han registrado casos en niños y adolescentes. Se reporta el caso clínico de un niño de 4 años de edad que concurre a la consulta en el año 2010 por pérdida prematura de piezas dentarias. La evaluación de los exámenes de rutina y las radiografías con las que acudió permitieron descartar una periodontitis como manifestación de una enfermedad sistémica. Se confirma el diagnóstico de periodontitis infantil agresiva, por medio de examen microbiológico y ténica PCR. El tratamiento indicado fue terapia básica, antibioticoterapia y controles periódicos. Luego de tre años no hay signos clínicos de la enfermedad. El diagnóstico preciso, sumado a la motivación constante y los controles periódicos, hacen posible la detención del avance de la enfermedad...

Cytotoxicity testing with three-dimensional cultures of transfected pulp-derived cells.

SV40 large T-antigen-transfected bovine pulp-derived cells were grown three-dimensionally on polyamide meshes. For optimal cell growth, various cell numbers and mesh coatings were tested. Next the three-dimensional cultures were used in a dentin barrier test device, and the system was evaluated by testing a set of dental filling materials. After 24 hr exposure with or without perfusion of the pulpal part of the test device, cell survival was evaluated using an MTT assay. In all experiments pulp-derived cells transfected with SV40 large T-antigen grew three-dimensionally on polyamide meshes and showed growth kinetics similar to those on cell culture plates with lag, log, and plateau phases (reached after about 14 days of incubation). Cross-sections of the three-dimensional cell cultures revealed about 15 to 20 cell layers. In vitro cytotoxicity tests resulted in cell survival rates which are in good agreement with in vivo data and with results obtained from cytotoxicity tests with three-dimensional cultures of human foreskin fibroblasts.

Concentrations of arsenic, cadmium, copper, lead, mercury, and zinc in human placentas from two cities in Ukraine.

Ukraine is a highly industrialized country with major environmental problems and deteriorating reproductive health. Heavy metals are known reproductive toxins; a study was undertaken to determine whether they were present at sufficient concentrations to be playing a major role in these health problems. Placental concentrations of arsenic, cadmium, copper, lead, mercury, and zinc were determined in 200 women from the general population of two urban areas of Ukraine, Kyiv and Dniprodzerzhinsk. Arsenic was detected in only 5% of the samples, lead in 22%, and mercury in 28%. Cadmium was detected in almost all samples, with a median of 5.2 ng/g. Concentrations of lead, mercury, and cadmium were low compared to those reported elsewhere, while zinc and copper concentrations were comparable.

[The characteristics of the local protective factors in disseminated suppurative peritonitis]. / Osobennosti faktorov mestnoi zashchity pri rasprostranennom gnoinom peritonite.

A comparative evaluation was done of factors of the local and systemic immunity in 18 patients with diffuse pyoperitonitis. Morphological (neutrophiles from the abdominal cavity in patients with peritonitis retrieve NBT in a diffuse-dotted fashion whereas neutrophiles of blood give a diffuse pattern and by granules) and functional (reduced metabolic activity of abdominal neutrophiles in a spontaneous NBT-test and an augmented index for the reserve versus metabolic activity of blood neutrophiles) differences in local and systemic factors of immune defence were noted. It is shown that the content of all classes of immunoglobulins is significantly lower in the peritoneal exudate than it is in blood serum. The above facts are to be considered in treatment of patients with generalized purulent peritonitis.

[Immunomodulator sensitivity in patients with peritonitis]. / Chuvstvitel'nost' bol'nykh s peritonitom k immunomoduliatoram.

The indexes of the loading rosetteforming tests were studied up in vitro in 10 healthy persons and in 12 patients with peritonitis. In the patients the hyporeactive type of reactivity and multidirectional reaction on immunomodulators was revealed more frequently by comparison with such in a healthy persons. The individual option of the immunocorrecting agents is mandatory in patients with an acute peritonitis.

[Evaluation of severity of the state of patients with peritonitis by the modified APACHE II system]. / Otsenka tiazhesti sostoianiia bol'nykh s peritonitom s ispol'zovaniem modifitsirovannoi sistemy APACHE II.

According to the APACHE II modified method 201 patient with an acute peritonitis was examined using the results of investigations available during the first 30 min after hospitalization of patient. Veritable differences of the modified APACHE II indexes were established in patients with local and diffuse peritonitis in dead and survived patients and in those who died in the first day and later after hospitalization.

[Complications and adverse effects of laser therapy in patients with suppurative-septic diseases]. / Oslozhneniia i pobochnye éffekty lazernoi terapii u patsientov s gnoino-septicheskimi zabolevaniiami.

The complications and side-effects of laser therapy in 229 patients with septic-purulent diseases were analyzed. Metal taste in oral cavity, sleepiness, vertigo, weakness, sense of heat were the most frequently occurring side-effects of laser therapy observed.

[Intraocular penetration of systemically administered cephalosporins under steady-state conditions in animal experiments]. / Intraokulare Penetration systemisch applizierter Cephalosporine unter Steady-state-Bedingungen im Tierversuch.

In previous studies of intraocular penetration of systemically applied antibiotics a single dose of the antibiotic was applied. An experimental study was performed in order to determine if maintaining a constant serum level for an extended period of time will lead to an increase in ocular concentration. The antibiotic dosage was calculated so that the serum level remained constant for a given period of time. Using the rabbit as an experimental animal two cephalosporins (Cefsulodin, Cefotiam) were then injected intravenously using an infusion pump. Evaluation of serum levels (high-performance liquid chromatography) revealed constant levels for each animal but different interindividual levels. The intraocular concentrations of Cefsulodin and Cefotiam were different. The intraocular level of Cefsulodin rose continuously over a period of 5 h, whereas the Cefotiam levels remained at the concentration measured after bolus injection.