ABSTRACT
Salacia crassifolia (Mart. Ex. Schult.) G. Don., popularly known in Brazil as "bacupari", "cascudo", and "saputá", is a shrub of the Celastraceae family that is unique to the Brazilian Cerrado region. In folk medicine, this plant has been mainly used to treat skin cancer and gastric ulcers. In the present study, the genotoxic, cytotoxic, antigenotoxic, and anticytotoxic effects of S. crassifolia stem bark fractions (hexane, ethyl acetate, and hydroalcoholic extracts) were evaluated using the mouse bone marrow micronucleus test. Our results showed that none of the S. crassifolia fractions led to a significant increase in the frequency of micronucleated polychromatic erythrocytes (MNPCE) (P > 0.05), suggesting the absence of genotoxicity. In the antigenotoxicity assessment, a significant decrease in the MNPCE frequency was observed in all fractions of this plant (P < 0.05), demonstrating its protective action against genotoxicity induced by mitomycin C (MMC), which was used as the positive control. Only the hexane fraction of S. crassifolia significantly decreased the poly- and normochromatic erythrocyte ratio (PCE/NCE) in all doses tested (P < 0.05), demonstrating its cytotoxic activity. In association with MMC, both ethyl acetate and hydroalcoholic fractions significantly increased the PCE/NCE ratio in almost all doses tested (P < 0.05), demonstrating the protective action of S. crassifolia against the cytotoxic effect of the positive control. In contrast, the hexane fraction presented a significant decrease in the PCE/NCE ratio in all treatments (P < 0.05), demonstrating an increase in this plant's cytotoxicity in mouse bone marrow cells.
Subject(s)
Cytotoxins/pharmacology , DNA Damage , Plant Bark/chemistry , Plant Extracts/pharmacology , Salacia/chemistry , Animals , Antibiotics, Antineoplastic/pharmacology , Bone Marrow Cells/drug effects , Chemical Fractionation , Cytotoxins/chemistry , Cytotoxins/toxicity , Erythrocytes/drug effects , Mice , Micronuclei, Chromosome-Defective/drug effects , Mitomycin/pharmacology , Plant Extracts/chemistry , Plant Extracts/toxicity , Plant Stems/chemistry , Salacia/anatomy & histologyABSTRACT
A família Myrtaceae possui representantes de grande interesse medicinal e o gênero Pimenta é um dos que merecem destaque. A maioria das espécies desse gênero é nativa da América Central, com exceção da Pimenta pseudocaryophyllus (Gomes) L. R. Landrum, nativa nas regiões centro-oeste e sudeste do Brasil. O presente estudo teve como objetivo revisar aspectos botânicos, químicos e farmacológicos descritos na literatura sobre o gênero Pimenta. Os dados foram obtidos de artigos originais e revisões indexadas nas bases Periódicos Capes, ISI Web of Knowledge, Bireme e SciELO. As espécies desse gênero podem ser arbustivas ou arbóreas, possuem pelos unicelulares, folhas usualmente coriáceas, inflorescência em dicásio ou panícula e são distinguidas, principalmente, pela estrutura do ovário. As propriedades farmacológicas são conferidas, principalmente, pelos óleos essenciais que são constituídos, na maioria, por derivados fenilpropanóides, monoterpenos, aldeídos monoterpênicos e alcoóis monoterpênicos. Dentre as propriedades farmacológicas apresentadas por espécies desse gênero destacam-se as anti-hipertensivas, anti-inflamatórias, analgésicas, antimicrobianas e antioxidantes. Das quinze espécies de Pimenta conhecidas, a Pimenta dioica (L.) Merrill e a Pimenta racemosa (Miller) J. Moore são as espécies de maior importância econômica e, por esse motivo são as mais estudadas do ponto de vista químico e farmacológico.
The family Myrtaceae has representatives of great medical interest, and the genus Pimenta deserves attention. Most species of this genus are native to the Central America, except Pimenta pseudocaryophyllus (Gomes) L. R. Landrum, which is native to the Central-West and Southeast Brazilian Regions. This study aimed to review botanical, chemical and pharmacological aspects described in the literature for Pimenta genus. Information was obtained from original papers and reviews indexed in the databases "Periódicos Capes", ISI Web of Knowledge, "Bireme" and SciELO. This genus can present shrubs or trees, which have unicellular hairs, usually coriaceous leaves and dichasium or panicle inflorescence, and are mainly distinguished by the ovary structure. The pharmacological properties are mostly due to essential oils, mainly consisted of phenylpropanoid, monoterpene, monoterpenic aldehyde and alcohol derivatives. Among these properties, antihypertensive, anti-inflammatory, analgesic, antimicrobial and antioxidant ones are highlighted. Of the fifteen known Pimenta species, Pimenta dioica (L.) Merrill and Pimenta racemosa (Miller) J. Moore have the greatest economic importance and consequently have been the most chemically and pharmacologically studied.
