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1.
An. acad. bras. ciênc ; 89(2): 1175-1188, Apr.-June 2017. tab, graf
Article in English | LILACS | ID: biblio-886685

ABSTRACT

ABSTRACT Detection of virulent strains associated with aquatic environment is a current concern for the management and control of human and animal health. Thus, Vibrio diversity was investigated in four estuaries from state of Ceará (Pacoti, Choró, Pirangi and Jaguaribe) followed by antimicrobial susceptibility to different antimicrobials used in aquaculture and detection of main virulence factors to human health. Isolation and identification were performed on TCBS agar (selective medium) and dichotomous key based on biochemical characteristics, respectively. Nineteen strains of genus Vibrio were catalogued. Vibrio parahaemolyticus (Choró River) and V. alginolyticus (Pacoti River) were the most abundant species in the four estuaries. All strains were submitted to disk diffusion technique (15 antimicrobials were tested). Resistance was found to: penicillin (82%), ampicillin (54%), cephalotin (7%), aztreonan (1%), gentamicin, cefotaxime and ceftriaxone (0.5%). Five pathogenic strains were chosen to verification of virulence factors. Four estuaries showed a high abundance of species. High number of tested positive strains for virulence is concerning, since some of those strains are associated to human diseases, while others are known pathogens of aquatic organisms.


Subject(s)
Vibrio/drug effects , Vibrio/pathogenicity , Drug Resistance, Multiple , Estuaries , Rivers/microbiology , Vibrio/isolation & purification , Virulence , Water Microbiology , Brazil , Microbial Sensitivity Tests , Virulence Factors , Aquatic Organisms/isolation & purification , Aquatic Organisms/drug effects , Aquatic Organisms/pathogenicity , Geographic Mapping , Anti-Bacterial Agents/pharmacology
2.
An Acad Bras Cienc ; 89(2): 1175-1188, 2017.
Article in English | MEDLINE | ID: mdl-28489191

ABSTRACT

Detection of virulent strains associated with aquatic environment is a current concern for the management and control of human and animal health. Thus, Vibrio diversity was investigated in four estuaries from state of Ceará (Pacoti, Choró, Pirangi and Jaguaribe) followed by antimicrobial susceptibility to different antimicrobials used in aquaculture and detection of main virulence factors to human health. Isolation and identification were performed on TCBS agar (selective medium) and dichotomous key based on biochemical characteristics, respectively. Nineteen strains of genus Vibrio were catalogued. Vibrio parahaemolyticus (Choró River) and V. alginolyticus (Pacoti River) were the most abundant species in the four estuaries. All strains were submitted to disk diffusion technique (15 antimicrobials were tested). Resistance was found to: penicillin (82%), ampicillin (54%), cephalotin (7%), aztreonan (1%), gentamicin, cefotaxime and ceftriaxone (0.5%). Five pathogenic strains were chosen to verification of virulence factors. Four estuaries showed a high abundance of species. High number of tested positive strains for virulence is concerning, since some of those strains are associated to human diseases, while others are known pathogens of aquatic organisms.


Subject(s)
Drug Resistance, Multiple , Estuaries , Rivers/microbiology , Vibrio/drug effects , Vibrio/pathogenicity , Anti-Bacterial Agents/pharmacology , Aquatic Organisms/drug effects , Aquatic Organisms/isolation & purification , Aquatic Organisms/pathogenicity , Brazil , Geographic Mapping , Microbial Sensitivity Tests , Vibrio/isolation & purification , Virulence , Virulence Factors , Water Microbiology
3.
Curr Microbiol ; 63(2): 126-30, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21626146

ABSTRACT

Between October 2008 and June 2009, 15 samples of 10 live oysters each (Crassostrea rhizophorae) measuring 8.31-10.71 cm were purchased from a restaurant on the seashore of Fortaleza, Brazil. The Vibrio count ranged from 75 (estimated) to 43,500 CFU/g. Fourteen species were identified among the 56 isolated Vibrio strains, with V. parahaemolyticus as the most prevalent. Two of the 17 V. parahaemolyticus strains were urease-positive and tdh- and trh-positive on multiplex PCR, but neither produced ß-hemolysis halos in Wagatsuma agar. Thus, fresh oysters served in natura in Fortaleza, Brazil, were found to contain Vibrio strains known to cause gastroenteritis in humans.


Subject(s)
Bacterial Proteins/genetics , Food Microbiology , Ostreidae/microbiology , Vibrio/genetics , Vibrio/isolation & purification , Virulence Factors/genetics , Animals , Bacterial Load , Brazil , Culture Media/chemistry , Hemolysin Proteins/metabolism , Hemolysis , Polymerase Chain Reaction , Vibrio/classification
4.
Braz. j. microbiol ; 35(1/2): 126-130, Jan.-Jun. 2004. ilus, tab
Article in English | LILACS | ID: lil-388810

ABSTRACT

O objetivo deste trabalho foi avaliar a qualidade microbiológica da ostra de mangue (Crassostrea rhizophorae) originária de um criadouro natural no estuário do Rio Cocó, Fortaleza, Ceará, Brasil. Para isso, foram realizadas as estimativas do Número Mais Provável (NMP) de Coliformes Totais (CT) e de Fecais (CF) e de Enterococcus spp. Os valores encontrados para CT e CF no músculo (com líquido intervalvar) variaram de <1,8 a >1.600 e <1,8 a 920 por grama, respectivamente. O valor do NMP de Enterococcus spp. variou de <3,0 a >1.100/g. Não houve correlação entre os parâmetros físico-químicos (temperatura, salinidade e pH) da água na área do criadouro e os níveis de contaminação encontrados nas ostras. Somente houve correlação entre os valores de CT e CF. Cepas de Enterococcus spp. foram isoladas e submetidas a testes bioquímicos para identificação das espécies e, posteriormente, foram testadas para verificar a produção de substância inibitória semelhante à bacteriocina utilizando a cepa-teste Escherichia coli ATCC 25922. De um total de 121 cepas de Enterococcus spp. testadas apenas uma, E. faecalis, apresentou atividade inibitória.


Subject(s)
Bacteriocins , Enterobacteriaceae , Enterococcus faecalis , Escherichia coli , In Vitro Techniques , Ostreidae , Culture Media , Methods
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