ABSTRACT
Maternal deprivation (MD) causes cognitive deficits that persist until adulthood. Thereby, the environmental enrichment (EE) is widely used to increase brain plasticity. Here, pregnant female rats were used and their offspring were submitted to neonatal MD from post-natal day 1-10; after weaning the rats were submitted to EE. MD caused deficits on short and long-term aversive and recognition memory and on cognitive flexibility tested on reversed Morris Water Maze test. MD also promoted the decrease of hippocampal Brain-Derived Neurotropic Factor (BDNF) protein expression. The EE was able to protect against the cognitive deficits, avoiding the memory and the cognitive flexibility disrupting, and normalizing hippocampal BDNF expression of rats submitted to MD. These data confirms that MD promotes long-life memory deficits and demonstrates that MD causes cognitive flexibility disruption; the mechanisms seem involve the decrease of BDNF. We also demonstrate that EE, which improves BDNF, is able to avoid memory deficits and cognitive flexibility disrupts.
Subject(s)
Brain-Derived Neurotrophic Factor/metabolism , Cognition/physiology , Hippocampus/metabolism , Housing, Animal , Maternal Deprivation , Memory/physiology , Animals , Animals, Newborn , Elevated Plus Maze Test , Memory Consolidation , Memory, Long-Term , Memory, Short-Term , Morris Water Maze Test , Neuronal Plasticity , Open Field Test , Rats , Recognition, PsychologyABSTRACT
Maternal deprivation (MD) in early life affects the development of the brain, causing cognitive losses in adulthood. Oxidative imbalance may be one of the factors that trigger these deficits. Therapies with antioxidant components, like green tea from Camellia sinensis (GT) has been used to treat or prevent memory deficits in a variety of conditions related to oxidative stress. Here we demonstrate that memory deficits caused by MD can be prevented by GT antioxidant activity in hippocampus. Pregnant female rats were used. Her puppies were submitted to MD and intake of GT. Recognition and aversive memory were evaluated, as well as hippocampal oxidative status. Data showed that MD prejudice short and long-term recognition and aversive memory and that GT protected memory. Hippocampal reactive oxygen species levels were increased in MD rats; this increase was avoided by GT supplementation. GSH was decreased on hippocampus MD rats. GT did not avoid GSH decrease, but promote the increase of total antioxidant capacity in MD rats' hippocampus. In conclusion, GT protects against memory deficits related to MD, and one of the implicated mechanism seems to be the antioxidant effects of GT.
Subject(s)
Camellia sinensis/metabolism , Maternal Deprivation , Memory Disorders/etiology , Memory Disorders/prevention & control , Acetylcholinesterase/metabolism , Age Factors , Analysis of Variance , Animals , Animals, Newborn , Camellia sinensis/chemistry , Catechin/metabolism , Chromatography, High Pressure Liquid , Exploratory Behavior/physiology , Female , Glutathione/metabolism , Lipid Peroxidation/physiology , Male , Maze Learning/physiology , Pain Threshold/physiology , Pregnancy , Rats , Reactive Oxygen Species/metabolism , Recognition, Psychology/physiologyABSTRACT
It is known from previous research that physical exercise prevents long-term memory deficits induced by maternal deprivation in rats. But we could not assume similar effects of physical exercise on short-term memory, as short- and long-term memories are known to result from some different memory consolidation processes. Here we demonstrated that, in addition to long-term memory deficit, the short-term memory deficit resultant from maternal deprivation in object recognition and aversive memory tasks is also prevented by physical exercise. Additionally, one of the mechanisms by which the physical exercise influences the memory processes involves its effects attenuating the oxidative damage in the maternal deprived rats' hippocampus and prefrontal cortex.
Subject(s)
Hippocampus/physiopathology , Maternal Deprivation , Physical Conditioning, Animal/physiology , Physical Conditioning, Animal/psychology , Prefrontal Cortex/physiopathology , Recognition, Psychology/physiology , Animals , Avoidance Learning/physiology , Glutathione/metabolism , Male , Memory Disorders/physiopathology , Memory Disorders/prevention & control , Memory, Long-Term/physiology , Memory, Short-Term/physiology , Oxidative Stress/physiology , Rats, Wistar , Reactive Oxygen Species/metabolism , Running/physiology , Running/psychology , Thiobarbituric Acid Reactive Substances/metabolismABSTRACT
Extinction is the learned inhibition of retrieval. Recently it was shown that a brief exposure to a novel environment enhances the extinction of contextual fear in rats, an effect explainable by a synaptic tagging-and-capture process. Here we examine whether this also happens with the extinction of another fear-motivated task, inhibitory avoidance (IA), and whether it depends on dopamine acting on D1 or D5 receptors. Rats were trained first in IA and then in extinction of this task. The retention of extinction was measured 24 h later. A 5-min exposure to a novel environment 30 min before extinction training enhanced its retention. Right after exposure to the novelty, animals were given bilateral intrahippocampal infusions of vehicle (VEH), of the protein synthesis inhibitor anisomycin, of the D1/D5 dopaminergic antagonist SCH23390, of the PKA inhibitor Rp-cAMP or of the PKC inhibitor Gö6976, and of the PKA stimulator Sp-cAMP or of the PKC stimulator PMA. The novelty increased hippocampal dopamine levels and facilitated the extinction, which was inhibited by intrahippocampal protein synthesis inhibitor anisomysin, D1/D5 dopaminerdic antagonist SCH23390, or PKA inhibitor Rp-cAMP and unaffected by PKC inhibitor Gö6976; additionally, the hippocampal infusion of PKA stimulator Sp-cAMP reverts the effect of D1/D5 dopaminergic antagonist SCH 23390, but the infusion of PKC stimulator PMA does not. The results attest to the generality of the novelty effect on fear extinction, suggest that it relies on synaptic tagging and capture, and show that it depends on hippocampal dopamine D1 but not D5 receptors.
Subject(s)
Extinction, Psychological , Fear , Hippocampus/metabolism , Receptors, Dopamine D1/metabolism , Animals , Anisomycin/chemistry , Behavior, Animal , Benzazepines/chemistry , Carbazoles/chemistry , Cyclic AMP/analogs & derivatives , Cyclic AMP/chemistry , Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors , Dopamine/chemistry , Learning , Male , Memory , Memory Disorders/metabolism , Protein Kinase C/antagonists & inhibitors , Rats , Rats, Wistar , Receptors, Dopamine D5/metabolism , Stress, Physiological , Thionucleotides/chemistry , Time FactorsABSTRACT
Este estudo tem a finalidade de relatar uma prática proposta (Semana do Cérebro de Uruguaiana) com o intuito de contribuir com a popularização e divulgação da Neurociência junto à Educação Básica. Paralelamente à Semana Internacional do Cérebro (Brain Awareness Week) foram realizadas atividades voltadas para alunos da rede pública de Educação Básica. As atividades foram organizadas em seis etapas: (1) divulgação; (2) palestra explicativa sobre o funcionamento do Sistema Nervoso Central; (3) exposição de neuroanatomia; (4) demonstração prática de ações nervosas; (5) noções de pesquisa científica em neurociências; e (6) avaliação. Ao final verificou-se a opinião dos alunos quanto às atividades realizadas durante as diferentes etapas, 98,6% manifestaram ter interesse em aprender mais sobre o sistema nervoso, 85,2% relataram que as atividades propostas serviram para despertar seu interesse por pesquisa e ciência, e, ao avaliarem as ações de uma forma geral, atribuindo uma nota de 0 a 10, a nota atribuída foi de 9,4 ± 0,99. Desse modo, pode-se afirmar que a proposta foi bem aceita pelos alunos, sendo considerada válida e atingindo o objetivo de promover a divulgação e popularização da neurociência.
This study aimed to report a practice (Brain Awareness Week of Uruguaiana) that was proposed with the aim of contribute with the popularization and dissemination of Neuroscience in the Basic Education. Alongside the Brain Awareness Week we performed activities with students from public basic education. The activities were organized in six steps: (1) disclosure; (2) lecture explaining the functioning of the Central Nervous System; (3) neuroanatomy exposition; (4) practical demonstration of nerve actions; (5) notions of scientific research neuroscience; and (6) evaluation. At the end was verified the opinion of students about the activities carried in the different steps. 98.6% of students expressed interest in learning more about the nervous system. 85.2% reported that the activities proposed in our actions served to awaken their interest on research and science, and when evaluating the actions in general, with a score from 0 to 10, the assigned score was 9.4 ± 0.99. Thus, we can claim that our proposal was well accepted by the students and is considered valid and reaching the aim of promoting the dissemination and popularization of neuroscience.
Subject(s)
Humans , Neurosciences , Human Experimentation , EducationABSTRACT
Este estudo tem a finalidade de relatar uma prática proposta (Semana do Cérebro de Uruguaiana) com o intuito de contribuir com a popularização e divulgação da Neurociência junto à Educação Básica. Paralelamente à Semana Internacional do Cérebro (Brain Awareness Week) foram realizadas atividades voltadas para alunos da rede pública de Educação Básica. As atividades foram organizadas em seis etapas: (1) divulgação; (2) palestra explicativa sobre o funcionamento do Sistema Nervoso Central; (3) exposição de neuroanatomia; (4) demonstração prática de ações nervosas; (5) noções de pesquisa científica em neurociências; e (6) avaliação. Ao final verificou-se a opinião dos alunos quanto às atividades realizadas durante as diferentes etapas, 98,6% manifestaram ter interesse em aprender mais sobre o sistema nervoso, 85,2% relataram que as atividades propostas serviram para despertar seu interesse por pesquisa e ciência, e, ao avaliarem as ações de uma forma geral, atribuindo uma nota de 0 a 10, a nota atribuída foi de 9,4 ± 0,99. Desse modo, pode-se afirmar que a proposta foi bem aceita pelos alunos, sendo considerada válida e atingindo o objetivo de promover a divulgação e popularização da neurociência.
This study aimed to report a practice (Brain Awareness Week of Uruguaiana) that was proposed with the aim of contribute with the popularization and dissemination of Neuroscience in the Basic Education. Alongside the Brain Awareness Week we performed activities with students from public basic education. The activities were organized in six steps: (1) disclosure; (2) lecture explaining the functioning of the Central Nervous System; (3) neuroanatomy exposition; (4) practical demonstration of nerve actions; (5) notions of scientific research neuroscience; and (6) evaluation. At the end was verified the opinion of students about the activities carried in the different steps. 98.6% of students expressed interest in learning more about the nervous system. 85.2% reported that the activities proposed in our actions served to awaken their interest on research and science, and when evaluating the actions in general, with a score from 0 to 10, the assigned score was 9.4 ± 0.99. Thus, we can claim that our proposal was well accepted by the students and is considered valid and reaching the aim of promoting the dissemination and popularization of neuroscience.
Subject(s)
Humans , Neurosciences , Human Experimentation , EducationABSTRACT
Este estudo tem a finalidade de relatar uma prática proposta (Semana do Cérebro de Uruguaiana) com o intuito de contribuir com a popularização e divulgação da Neurociência junto à Educação Básica. Paralelamente à Semana Internacional do Cérebro (Brain Awareness Week) foram realizadas atividades voltadas para alunos da rede pública de Educação Básica. As atividades foram organizadas em seis etapas: (1) divulgação; (2) palestra explicativa sobre o funcionamento do Sistema Nervoso Central; (3) exposição de neuroanatomia; (4) demonstração prática de ações nervosas; (5) noções de pesquisa científica em neurociências; e (6) avaliação. Ao final verificou-se a opinião dos alunos quanto às atividades realizadas durante as diferentes etapas, 98,6% manifestaram ter interesse em aprender mais sobre o sistema nervoso, 85,2% relataram que as atividades propostas serviram para despertar seu interesse por pesquisa e ciência, e, ao avaliarem as ações de uma forma geral, atribuindo uma nota de 0 a 10, a nota atribuída foi de 9,4 ± 0,99. Desse modo, pode-se afirmar que a proposta foi bem aceita pelos alunos, sendo considerada válida e atingindo o objetivo de promover a divulgação e popularização da neurociência
This study aimed to report a practice (Brain Awareness Week of Uruguaiana) that was proposed with the aim of contribute with the popularization and dissemination of Neuroscience in the Basic Education. Alongside the Brain Awareness Week we performed activities with students from public basic education. The activities were organized in six steps: (1) disclosure; (2) lecture explaining the functioning of the Central Nervous System; (3) neuroanatomy exposition; (4) practical demonstration of nerve actions; (5) notions of scientific research neuroscience; and (6) evaluation. At the end was verified the opinion of students about the activities carried in the different steps. 98.6% of students expressed interest in learning more about the nervous system. 85.2% reported that the activities proposed in our actions served to awaken their interest on research and science, and when evaluating the actions in general, with a score from 0 to 10, the assigned score was 9.4 ± 0.99. Thus, we can claim that our proposal was well accepted by the students and is considered valid and reaching the aim of promoting the dissemination and popularization of neuroscience
Subject(s)
Humans , Neurosciences , Human Experimentation , EducationABSTRACT
Este estudo tem a finalidade de relatar uma prática proposta (Semana do Cérebro de Uruguaiana) com o intuito de contribuir com a popularização e divulgação da Neurociência junto à Educação Básica. Paralelamente à Semana Internacional do Cérebro (Brain Awareness Week) foram realizadas atividades voltadas para alunos da rede pública de Educação Básica. As atividades foram organizadas em seis etapas: (1) divulgação; (2) palestra explicativa sobre o funcionamento do Sistema Nervoso Central; (3) exposição de neuroanatomia; (4) demonstração prática de ações nervosas; (5) noções de pesquisa científica em neurociências; e (6) avaliação. Ao final verificou-se a opinião dos alunos quanto às atividades realizadas durante as diferentes etapas, 98,6% manifestaram ter interesse em aprender mais sobre o sistema nervoso, 85,2% relataram que as atividades propostas serviram para despertar seu interesse por pesquisa e ciência, e, ao avaliarem as ações de uma forma geral, atribuindo uma nota de 0 a 10, a nota atribuída foi de 9,4 ± 0,99. Desse modo, pode-se afirmar que a proposta foi bem aceita pelos alunos, sendo considerada válida e atingindo o objetivo de promover a divulgação e popularização da neurociência.
This study aimed to report a practice (Brain Awareness Week of Uruguaiana) that was proposed with the aim of contribute with the popularization and dissemination of Neuroscience in the Basic Education. Alongside the Brain Awareness Week we performed activities with students from public basic education. The activities were organized in six steps: (1) disclosure; (2) lecture explaining the functioning of the Central Nervous System; (3) neuroanatomy exposition; (4) practical demonstration of nerve actions; (5) notions of scientific research neuroscience; and (6) evaluation. At the end was verified the opinion of students about the activities carried in the different steps. 98.6% of students expressed interest in learning more about the nervous system. 85.2% reported that the activities proposed in our actions served to awaken their interest on research and science, and when evaluating the actions in general, with a score from 0 to 10, the assigned score was 9.4 ± 0.99. Thus, we can claim that our proposal was well accepted by the students and is considered valid and reaching the aim of promoting the dissemination and popularization of neuroscience.
