[Effect of spectra on growth of chlorella and isochrysis].

Focusing on the speed and efficiency, the effects of different spectra on the growth of chlorella vulgaris and isochrysis galbana Parke 8701 were investigated by using monochromatic LED (light-emitting diode) and fluorescent lamp as light sources. It was concluded that continuous spectra accelerate the top-growth-rate, blue light has the best efficiency, and the combination of them can obtain a good balance of speed and efficiency. For the purpose of measuring spectra as a parameter of irradiation quantitatively, spectra-absorbability-coefficient defined as the quanta-absorbability- efficiency of spectra for algae was calculated by means of absorption spectra of algae and emission spectra of light sources. Compared with the experimental results the coefficients of different light sources have a positive correlation to their efficiency for growth, so the coefficient can be used to elementarily quantify the relation between the spectra and the efficiency for growth.

[2+1, 1+1 and 1+1+1 multiphoton resonant ionization of manganese atom].

Resonance ionization spectroscopy (RIS) is an important technique for trace analyses. In the present paper, based on the population rate equations, "2+1", "1+1" and "1+1+1" multiphoton resonant ionization processes of Mn atom are studied by computer simulation. The phenomena and the threshold condition of saturated excitation and ionization processes are discussed. The threshold laser intensities for saturated ionization of Mn atom with different RIS schemes are calculated. With the typical Q-switched laser duration of 10 ns, the obtained saturation threshold laser intensity for the ionization steps in all the resonant ionization processes of Mn is about 10(8) W x cm(-2) with the exception of the "1+ 1" resonant ionization of Mn which is 3 orders of magnitude lower. The obtained threshold laser intensity for the saturated excitation steps is about 10(2)-10(3) W x cm(-2) in "1+1" and "1+1+1" ionization processes. It is also shown that the threshold laser intensity required for saturated excitation and ionization processes turns smaller with the laser duration prolonged.

[Investigation of calculating the FRET efficiency of donor-acceptor pair].

The research on the method of calculating FRET efficiency is important to organic white LED research. In the present article, a new method of calculating the FRET efficiency of donor-acceptor pair by means of emission and excitation spectra was investigated. This method is easy to use to process data, needs only normal experiment equipments and doesn't need the parameters such as fluorescence quantum yield. However it can only be used to calculate comparative result. With this method, the FRET efficiency of fluorescein sodium, alpha-naphthol, bromocresol purple and riboflavin with different concentrations was studied, and the results match the experimental results completely, so this method has proved to be reasonable.

[Applying patial least-squares discriminant analysis on autofluorescence spectra to identify gastric cancer].

Measurement of fluorescence intensity was performed at excitation wavelength of 308 nm and emission wavelength in the range of 328-596 nm. The partial least-squares (PLS) method was used to analyze autofluorescence spectra of gastric cancer. The 42 normal samples and 42 cancer samples were taken from 42 gastric cancer patients. The normalized and centralized spectra of two kinds of samples showed similar but divergent patterns. PLS classification algorithm could differentiate cancer tissues from normal tissues with a sensitivity of 83.3%, a specificity of 95.2%, and a positive predictive value of 94.6%. We concluded therefore that the PLS method was a fast, effective choice for identification of gastric cancer.

[Study of achieving white organic LED by fluorescence dye].

Some hybrid organic LEDs are made by using fluorescence dye and InGaN blue-light chip and the possibility of achieving white organic LED is investigated according to light conversion theory. Firstly, the LEDs made by normal method and double-dotting glue method are studied. It is found that the double-dotting glue method is too complex and the LEDs made by this way is low-luminance, so it isn't fit to make LED. Secondly the different weight ratio of 1/1/100, 1/1/200, 2/1.5/100 ax-17/zq-13/AB LEDs are manufactured. The 2/1.5/100 LED's color coordinate is (0.32, 0.30), approaching to white point and the correlative color temperature is 6290K which is close to sunlight. So it is a relatively ideal white lamp-house.

[Study of luminescence ability of organic white light emitting diode by light conversion].

An organic luminescence material of white light was investigated. The organic luminescence film was made by heating and melting method. The film consists of riboflavin, anthrone derivative, and linear low-density polyethylene. The results show that the spectra of luminescence films were affected by weight ratio and heating time of the made films. The spectra were wide band from green light to red light. The organic white light emitting diodes made of this film have super performance.

[The study of protoporphyrin IX metabolism in cell proliferation using yolk as the target samples].

In order to find the better target sample to investigate protoporphyrin IX metabolism in the cell proliferation process in chick embryos, the emission spectra of egg white, yolk and urine from non-incubated and incubated eggs were measured under the excitation at 405 nm. It was observed that, among the three components investigated, only yolk sample was possible to get in pureness almost through the whole incubation process. Furthermore, compared with the egg white, the yolk had more obvious characteristic emission of protoporphyrin IX and lower background spectra and turned to be the better target sample for the investigation. The relative characteristic emission intensity of PpIX of yolk samples was used to determine the level of PpIX metabolism. It was found that the relative emission intensity increases during the first 10 days of proliferation process, indicating an increase in the concentration or in the metabolism level of protoporphyrin IX. The relative emission intensity, hence the PpIX metabolism level, then decreased gradually during the rest days of proliferation process. These results are similar to those obtained on human malignant tumors as they develop. The result testifies the point of view further that the abnormity of PpIX metabolism is correlative to the rapid limitless cell proliferation.

[Research on energy transfer between riboflavin and anthrone derivative].

According to Forster's theory about energy transfer, the energy transfer between riboflavin and anthrone derivative was investigated. It was found that there is a notable energy transfer between riboflavin and anthrone derivative, and the energy transfer efficiency is affected by the following factors: heating temperature, heating time, and mass ratio. And the best ratio of riboflavin and anthrone derivative is 1:3. At the same time the energy transfer efficiency drops with increasing heating temperature and time.

[Fluorescence study by simulating the metabolizability of carotenoid and porphyrin during cancer development].

The purpose of the present work was to investigate the effects of carotenoid on the spectroscopic examination of human blood serum by simulating the metabolizability of the carotenoid and porphyrin during the development of cancer and by studying the fluorescence of them. Blood serum was prepared with blood sample from elbow vein and the luminescence center of blood serum was studied using photo induced-luminescence techniques. The major factors of blood serum fluorescence were electron transitions in serological protein, carotenoid and porphyrin molecules with conjugated groups. The intensity of the fluorescence spectrum at 530 nm was enhanced while the minimum intensity at 614 nm was also enhanced with the content of carotenoid increasing, thus the value of diagnostic symbol I decreased, namely the veracity of cancer examination decreased.

[The reformation of tomato ecology using photo-ecology film].

The action spectrum of photosynthesis of tomato was studied with excitation and fluorescence spectra of its leaf. To simulate action spectrum of tomato the fluorescent auxiliary was specially prepared and used to make the photo-ecology film. The film was used to cover the roof of greenhouses for planting tomatoes in Jianguo village of Xinfa town, Daoli district, Harbin. The survey of ecology conditions, tomato growth and yield has been made in comparison with those in the greenhouse using normal film. It is shown that the yield of tomato in the photo-ecology film greenhouse has increased by 53.7%, which leads to a production value rise of 61.4%.

[The non-trauma fluorescence analysis of the porphyrin in the blood of human superficial skin tissue].

The porphyrin compound in human body mainly includes hemoglobin, protoheme, protoporphyrin, coproporphyrin, uroporphyrin and so on. As we know, the metabolism of the porphyrin in human body depends on the physical conditions. It was found that the relative content of protoporphyrin (PPIX) and carotenoid in blood varied with the developing of the malignant tumor. A system, including Ti: sapphire laser, optical fiber delivery system, fluorescence probe, spectrometer CCD and computer, has been developed to examine the porphyrin in human superficial skin tissue without trauma. It was shown that the fluorescence spectrum of the porphyrin obtained from the superficial skin tissue of human earlobe has the same feature as that from the aqueous solution with PP IX.

[Influence of initial chirp on the evolution of spectra].

The influence of dispersion and nonlinearity on the spectra was analysed. The nonlinear Schrödinger equation was solved numerically in the frequency domain. The effect of the initial chirp on the spectra of different formats was simulated. It was found that the influence of the initial chirp on the spectra changed with different formats. The positive initial chirps make spectra spreading and negative initial chirps have little influence upon spectra for Gauss pulses. For super Gauss pulses, the spectra have some side tails, which cause energy loss, but the initial chirp has very little effect on the spectra. The initial chirps have a great influence on the spectra for soliton pulse, especially for high order soliton, which suggests that initial chirp must be decreased for stable pulse transmission.

[The acting spectra simulating and planting of high yield radish].

The acting spectra for the photosynthesis of radish were investigated by the reflection spectra of radish leaves. It was found that the reflection spectrum of radish leaves and the acting spectrum required for photosynthesis were similar to a reflection peak at 550 nm and two absorption bands around wavelengths of 440 and 680 nm. The fluorescent auxiliary and colorant were dispersed in macromolecule resin to make the photo-ecology agricultural film, hence simulating the acting spectra of crops. The fluorescence spectrum of photo-ecology film showed two emission bands at the wavelengths of 616 and 660 nm which overlap the band of 680 nm in the acting spectrum. In the excitation spectra of the film, a peak was found at the wavelength of 550 nm, the same as the reflection band of radish leaves. As a result of this, the solar radiation in the wavelength range of red could be enriched by using the photo-ecology film. It was shown that the yield of radish, planted in the greenhouse of the photo-ecology film, had increased by 20.2%.