ABSTRACT
The instability of the ocean waves, such as intermittence, randomness, and irregularity, greatly affects the application of a triboelectric nanogenerator (TENG) in its aspects and leads to the irregularity and uncontrollability of its output performance. Hence, the energy storage TENG (ES-TENG) based on the ratchet mechanism is proposed in this work. The ES-TENG uses the ratchet mechanism to store the wave energy in the clockwork spring and then releases it in a centralized way to convert the wave energy into electric energy. When the ES-TENG adopts this method, the change of external excitation does not affect its output performance. Simultaneously, the shell of the ES-TENG is duck-shaped, which can better adapt to the wave environment. The peak power, open-circuit voltage, and short-circuit current of the ES-TENG are 6.2 mW, 495 V, and 19 µA, respectively. In the simulated wave experiment, the ES-TENG can successfully drive a temperature sensor. In summary, this work shows an economic, environmental friendly TENG that can adapt to the wave motion, and its output performance is not affected by wave instability, which has an important guiding significance for the further development and utilization of TENG in ocean energy.
ABSTRACT
Label-free quantitative proteomic (LFQ) and widely targeted metabolomic analyses were applied in the safety evaluation of three genetically modified (GM) maize varieties, BBL, BFL-1, and BFL-2, in addition to their corresponding non-GM parent maize. A total of 76, 40, and 25 differentially expressed proteins (DEPs) were screened out in BBL, BFL-1, and BFL-2, respectively, and their abundance compared was with that in their non-GM parents. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that most of the DEPs participate in biosynthesis of secondary metabolites, biosynthesis of amino acids, and metabolic pathways. Metabolomic analyses revealed 145, 178, and 88 differentially accumulated metabolites (DAMs) in the BBL/ZH58, BFL-1/ZH58, and BFL-2/ZH58×CH72 comparisons, respectively. KEGG pathway enrichment analysis showed that most of the DAMs are involved in biosynthesis of amino acids, and in arginine and proline metabolism. Three co-DEPs and 11 co-DAMs were identified in the seeds of these GM maize lines. The proteomic profiling of seeds showed that the GM maize varieties were not dramatically different from their non-GM control. Similarly, the metabolomic profiling of seeds showed no dramatic changes in the GM/non-GM maize varieties compared with the GM/GM and non-GM/non-GM maize varieties. The genetic background of the transgenic maize was found to have some influence on its proteomic and metabolomic profiles.
ABSTRACT
The guy cables can effectively improve the bearing capacity for the telescopic boom of the crane, while the introduction of tensioned cables makes the buckling analysis complicated. The primary objective of this study was to propose an analytical method for the out-of-plane buckling of the telescopic boom with the spatial symmetric guy cables. To analyze the influence of the guy cables on the out-of-plane buckling property of the telescopic boom, the deflection differential equation of the multi-stepped telescopic boom with guy cables was established based on the theory of elastic beam, then the buckling characteristic equation to determine the critical load of the telescopic boom was derived. Comparison of results with that given by the finite element method showed the high accuracy of the proposed method. In the end, with this equation, the influences of the structural geometric parameters on the critical load were investigated. The results indicated that, in the engineering application, the critical load of the telescopic boom can be increased by decreasing the length ratio a/L or increasing the angle φ between the two cables. The influence of angle θ on the out-of-plane buckling analysis of the telescopic boom can be neglected. With the proposed method, the buckling behavior of the telescopic boom with guy cables can be solved accurately. The present work is significant to structural design and safety analysis of the telescopic boom, and it can be utilized to provide technical support for the structural design of telescopic boom.
ABSTRACT
PURPOSE: Podocyte injury is a key event in proteinuric kidney disease and eventually glomerular scarring. While adrenomedullin (AM), a potent vasodilatory peptide, has been reported to confer renoprotection in several experimental models of kidney diseases, its effect on injured podocytes and the related mechanism is still largely unknown. METHODS: Employing Western blotting analysis, immunoprecipitation and immunofluorescence, we investigated the effects of AM on the expressions of podocyte cytoskeletal proteins and Rho-family small GTPases (Rho GTPases) in puromycin aminonucleoside (PAN)-induced podocyte injury, both in cultured podocytes and in PAN nephrosis rats. Urinary protein excretion and the morphologic changes of kidney in PAN nephrosis rats were evaluated. Glutathione-S-transferase pull-down assay was applied for Rho GTPases activity. RESULTS: PAN induced massive albuminuria and morphologic injury, which were significantly mitigated by AM administration. AM significantly antagonized not only the PAN-decreased expressions of synaptopodin, nephrin, CD2AP and podocin, but also the PAN-disrupted interactions between synaptopodin-RhoA, nephrin-CD2AP, and CD2AP-Rac1-cortactin. These effects of AM in cultured podocytes were mostly significantly blocked by H89, a PKA inhibitor. AM dramatically upregulated the PAN-induced Rho GTPases protein expressions and their activities. PAN increased the expressions of endogenous AM and its receptor RAMP2 which was furthermore upregulated by AM administration. CONCLUSIONS: AM alleviated podocyte injury induced by PAN both in cell culture and in PAN nephrosis. The beneficial effects of AM on podocytes can be attributable to direct modulation of podocyte cytoskeletal proteins and Rho GTPases, mainly via a PKA-dependent pathway.
