ABSTRACT
A Gram-reaction-negative, strictly aerobic, non-pigmented, non-gliding, rod-shaped bacterium, designated strain BY4(T), was isolated from freshwater. Cells were catalase- and oxidase-positive and indole was produced. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BY4(T) belonged to the family Flavobacteriaceae and showed 91.6-95.9% sequence similarities to the most closely related strains. The major respiratory quinone was MK-6 and the major polar lipid was phosphatidylethanolamine. The major polyamine was homospermidine and the major fatty acids were iso-C(15â:â0), iso-C(17â:â0) 3-OH and summed feature 3 (C(16â:â1)ω7c and/or C(16â:â1)ω6c). The DNA G+C content was 30.0 mol%. On the basis of phenotypic, phylogenetic and genotypic features, strain BY4(T) is suggested to represent a novel species in a new genus within the family Flavobacteriaceae, for which the name Chishuiella changwenlii gen. nov., sp. nov. is proposed. The type strain of this type species is BY4(T) (â=âCGMCC 1.12707(T)â=âJCM 19633(T)). On the basis of data collected from previous and present studies, it is proposed to reclassify Wautersiella falsenii to the genus Empedobacter as the new combination Empedobacter falsenii comb. nov. (type strain NF 993(T)â=âCCUG 51536(T)â=âCIP 108861(T)).
Subject(s)
Flavobacteriaceae/classification , Fresh Water/microbiology , Phylogeny , Bacterial Typing Techniques , Base Composition , China , DNA, Bacterial/genetics , Fatty Acids/chemistry , Flavobacteriaceae/genetics , Flavobacteriaceae/isolation & purification , Molecular Sequence Data , Phosphatidylethanolamines/chemistry , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Spermidine/chemistry , Vitamin K 2/analogs & derivatives , Vitamin K 2/chemistryABSTRACT
A Gram-reaction-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, AY17T, was isolated from the Chishui River in Guizhou Province, South-west China. Strain AY17T grew optimally at pH 7.0 and 20 °C. Flexirubin-type pigments were produced. 16S rRNA gene sequence analysis indicated that strain AY17T belonged to the family Chitinophagaceae within the phylum Bacteroidetes; the closest phylogenetic relative was Taibaiella smilacinae PTJT-5T (95.3% gene sequence similarity). The DNA G+C content was 49.5 mol%. Strain AY17T contained MK-7 as the predominant respiratory quinone and phosphatidylethanolamine as the major polar lipid. The major fatty acids were iso-C15:0, iso-C15:1G and iso-C17:0 3-OH. On the basis of phylogenetic, phenotypic and genetic data, strain AY17T was classified as representing a novel species of the genus Taibaiella, for which the name Taibaiella chishuiensis sp. nov. is proposed. The type strain is AY17T (=CGMCC 1.12700T=JCM 19637T).
