[Mice scald model investigated by OCT combined with reflection spectrum].

OCT combined with reflective spectrum was employed to precisely evaluate the mice scald model. Under the conditions that the temperature increased by 5 degrees C from 60 to 95 degrees C with 20 s thermal effect, the cutis depth linearly increased. The fitting absorption parameters and the gradients showed the V trends. Wave peaks of first order differential were legible from 65 to 85 degrees C, then wave peaks became worse over 85 degrees C. At color coordinates, red value decreased severely from 65 to 85 degrees C and from 90 to 95 degrees C, green value continuously decreased, but blue value increased. Under the conditions that thermal effect time increased from 10 to 40 s at 90 degrees C, the main changes of above factors happened at about 20 s, and after 20 s effect time the change of these factors was weak. These factors could reflect the form and progresses of zones of coagulation, injury of cutis and hypodermis. So OCT combined with reflection spectrum could provide a novel method that can be applied for the real-time, low-cost, in vivo and noninvasive optical biopsy on scald.

[Comparative study of reflectance spectroscopy of women's acupoints around menstruation].

Acupoint was reaction of viscera and its optical parameter was an important characteristic of tissue. In the present work, in order to discuss specificity of acupuncture on the response of qi and blood in human body, we compared the diffuse reflectance of Taichong (LV3), Taibai (SP3), and Chongyang (ST42) before, during and after menstruation. All the acupoints had the same shape of the spectrum and troughs were all at 423.16, 544.06 and 577.47 nm. The values of reflectance during menstruation were greater than before and after menstruation, especially for SP3 and ST42 (P < 0.05). SP3 and ST42 were more sensitive to the changes of qi and blood than LV3, and it was asymmetric and we found no evidence for the imbalance of the left side and right. These results indicated that acupoint diffuse reflectance changed with qi and blood, and that SP3 and ST42 had close relationship with menstruation. Diffuse reflectance may be possibly used in the quantitative analysis of qi and blood.

[Analysis of polyA, polyU and double-stranded complex polyA x polyU via Raman spectroscopy].

The Raman spectra of PolyA, PolyU and their double-stranded complex were measured, and the spectral changes upon the formation of double-stranded complex were studied. The experimental results show: (1) Under the experimental conditions used in the present work (0.14 mol x L(-1) NaCl, 1 mmol x L(-1) Tris solution, neutral pH and 15 degrees C), PolyU, PolyA and PolyA x PolyU occur as random-coiled, A-single-stranded helical and A-double-stranded helical conformations, respectively. One of the main spectral differences between the latter two conformations and the former one is the Raman band near 814 cm(-1) of ordered structures. Another difference is in the full width at half the maximum (i.e. FWHM) of the band near 1100 cm(-1). The FWHM of the band 1100 cm(-1) of PolyA is the same as that of PolyA x PolyU, while the band of PolyU shows remarkable broadening. In addition, we found that the conformation of PolyA is somewhat not so ordered as that of its duplex, which can be concluded from the value of I814/I1100 of the two polynucleotides. (2) The formation of duplex makes base-base stacking interactions much stronger, and the conformation of the backbone more ordered, which leads to obvious Raman hypochromic effect with some corresponding band shift. In this process, PolyU underwent more significant spectral changes than PolyA. As spectral markers, these results can be of great importance in Raman spectral signal detection of gene-chips.