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Objective:To explore the composition and content differences of volatile oil in the leaves of Phlomis umbrosa from different habitats, in order to clarify the chemical composition and content on efficacy.Methods:To collect the Phlomis umbrosa from Haoping area in Mei county and Sanyuan county of Shaanxi province. The volatile oil of Phlomis umbrosa was extracted by steam distillation method. Gas chromatography- mass spectrometry (GC-MS) was used to detect the chemical constituents of volatile oil.Results:Forty-nine kinds of volatile components were identified from the leaves collected in Haoping area of Mei county and fifty-six volatile component were identified from the leaves collected in Sanyuan county. There are five ingredients in common, excluding diacetone alcohol. 2-tert-butoxy-5-methylthiophene is the main volatile oil composition and the content reached 18.009% of leaves collected from Haoping area in Mei county, while paclitaxel is main volatile oil composition and the content reached 50.728% in Sanyuan county.Conclusions:The chemical compostion and content of volatile oil of Phlomis umbrosa in different habitats are different.
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Objective:To explore the difference of constituents and contents of volatile oil in Perilla leaf and Perilla stem.Methods:The volatile oils of Perillaleaf and Perillastems were extracted with steam distillation method. Gas chromatography-mass spectrometry (GC-MS) technique was used to detect the chemical constituents of volatile oil from different parts of Perilla frutescens. The relative percentage content of each component was determined by peakarea normalization method, and the differences of the components and contents of volatile oils from different parts of Perilla frutescens were compared.Results:A total of 24 volatile oil components were identified in perilla leaves and 19 volatile oil components in Perilla stems. The most components in Perilla leaf and Perilla stems was 2-Hexanoyl furan. The content of 2-Hexanoyl furan in the oil of Perilla leaf reached 67.493%. The content of 2-Hexanoyl furan in the oil of Perilla stem reached 70.473%.Conclusions:The composition and content of volatile oil in different parts of Perillafrutescens are different.
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Qinling is a treasury of medicine with abundant resources of herbs. Due to the goal of enriching teaching contents, quality of teaching and creating a Chinese materia medica teaching system. Based on the Qinling plant gene database, our education team presents a series of innovative thoughts, such as multidisciplinary contact, inheriting and carrying forward the folk medicine, attaching importance to the practical application of herbs in Qinling and theory with practice to enrich teaching contentsand creating Shanxi characteristic teaching system of Chinese materia madica.That makes a contribution to raise the level of teaching in modern traditional Chinese medicine and promote the development of medicine in Shannxi.
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Chinese medicine talents are the foundation and guarantee for the development of TCM, and the first resource for the inheritance and innovation of traditional Chinese medicine. Reforming the curriculum system and training mode of Chinese medicine, training Chinese medicine professional who are suitable for research and development of Chinese medicine, such as cultivation of Chinese medicine materials, quality detection, species identification and resource census, is the direction of further expansion and reformation of Chinese medicine education. In this article, on account of the characteristic of Chinese medicine professional courses, we will combine practice education mode with professional knowledge competition, to probe innovative mode of comprehensive ability training for Chinese medicine based on the combination of"Multi-mode and Multi-Ways". The new mode of Chinese medicine education is to provide reference for trainers of Chinese medicine.
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Objective To investigate the optimal isolation and purification conditions of flavonoids in Gnaphlium affine Thunb. and the content of flavonoids in different parts of Gnaphlium affine Thunb. was measured. Methods The separation and purification abilities of D-101 macroporoue adsorbing resins for flavonoids in Gnaphlium affine Thunb. were studied with adorption and desorption as index. The static adsorption and dynamic adsorption methods were used to analyze the effects of static saturated adsorption, static elution rate, sample concentration, sample pH value, eluent concentration and amount of eluent. The flavonoids concentrations were determined with rutin as standard. Results The D-101 macroporous adsorption resin had good effect on the separation and purification of flavonoids from Gnaphlium affine Thunb.; The optimal conditions for purification were: sample concentration 1.0 mg/ml, sample pH=4, 60％ ethanol as desorption solvent, washing flow 2 BV/h, with these parameters. With such condition, the purity of flavonoids in Gnaphlium affine Thunb. was 60.92％; The content of flavonoids in Gnaphlium affine Thunb. showed the highest content was in the stem, the second in the flower, and the least in the root. Conclusions The purification of flavonoids from the D-101 macroporous adsorption resin increased in the best purified separation conditions and the content of flavonoids was the highest in the leaf.