[Effect of antipsychotic amisulpride on immune reactivity].

The effect of atypical antipsychotic solian (amisulpride), binding predominantly to dopamine D2/D3-receptors, on the immune reactivity has been studied in mice of the CBA strain with different psychoemotional states (aggressive and submissive behavior). In addition, the effect of solian on the expression of various CD-markers of lymphocytes in has been analyzed in vitro for patients with schizophrenia diagnosis. Chronic (10 days) administration of solian in mice at a dose of 5.0 mg/kg resulted in a significant suppression of the immune response to T-dependent antigen (sheep red blood cells). This effect was manifested in animals with both psychoemotional states, but was more expressed in aggressive animals. In the in vitro system, solian produced opposite effects on the expression of surface CD receptors in lymphocytes of patients with schizophrenia. It is suggested that solian does not only affects immune function through D2 receptors of the brain, but also directly influences immunocompetent cells.

[Enzyme immunoassay in diagnosis of opisthorchiasis. Communication 2. Enzyme immunoassay test system for diagnosis of acute opisthorchiasis]. / Immunofermentnyi analiz v diagnostike opistorkhoza. Soobshchenie 2. Kharakteristika immunofermentnoi test-sistemy dlia diagnostiki ostrogo opistorkhoza.

A standard, highly specific and sensitive enzyme immunoassay test system has been developed for the diagnosis of acute opisthorchiasis, which is based on indirect solid-phase enzyme immunoassay for determination IgM antibodies to the antigen derived from Opisthorchis felineus marita extracts. The sensitivity of the test system is 87%, its specificity is 96.8%.

[Immunoenzyme analysis in the diagnosis of opisthorchiasis. I. The development of an immunoenzyme method for determining IgM antibodies to the Opisthorchis antigen]. / Immunofermentnyi analiz v diagnostike opistorkhoza. Soobshchenie I. Razrabotka immunofermentnogo metoda opredeleniia IgM-antitel k opistorkhoznomu antigenu.

An assay was developed to determine IgM antibodies to Opisthorchis felineus antigens for the diagnosis of acute infection. The method is based on indirect solid-phase enzyme immunoassay. Optimal conditions were elaborated for analysis and its high specificity and specifications are shown in the paper.

[Cross-reacting antigens of Opisthorchis felineus and Salmonella typhi and their possible effect on the clinical course of typhoid fever]. / Perekrestno reagiruiushchie antigeny Opisthorchis felineus i Salmonella typhi i ikh vozmozhnoe vliianie na klinicheskoe techenie briushnogo tifa.

The experiments on rabbits immunized with a complex O. felineus antigen and spirit abdominal typhoid vaccine enriched with Vi-antigen, as well as studies on patients with chronic opisthorchiasis and bacterial carriers of S. typhi have established the presence of cross-reacting antigens in abdominal typhoid and opisthorchiasis pathogens. It is suggested that these antigens play an essential role in the development and outcome of abdominal typhoid secondary to chronic opisthorchiasis invasion.

[Circulating immune complexes and insulin antibodies in obesity in children]. / Tsirkuliruiushchie immunnye kompleksy i antitela k insulinu pri ozhirenii u detei.

Clinical and laboratory studies of circulating immune complexes (CIC) and insulin antibodies were carried out in 110 children aged 7 to 14 years. Of these, 90 patients suffered from obesity and 20 were normal. The data obtained indicate that obese children had a high content of CIC and insulin antibodies. The high content of CIC and appearance of pathogenic CIC in obese children may attest to a possibility of the development of autoimmune processes. Insulin resistance seen in obese children is likely to be connected with appearance of insulin antibodies.

[Humoral factors in children with diabetes mellitus]. / Gumoral'nye faktory pri sakharnom diabete u detei.

Clinical and laboratory studies into the physiochemical properties of circulating immune complexes, serum IgA, IgM, IgG and antibodies against insulin contained by blood serum and circulating immune complexes were conducted in 102 children aged 7-14 years. Of these, 60 children were affected with insulin-dependent diabetes mellitus (IDDM) and 42 were normal. The data obtained point to the activity of autoimmune processes in IDDM patients in the first years of the disease, which manifested itself by the high content of circulating immune complexes and antibodies against insulin. The content of circulating immune complexes may serve an additional immunological indicator of compensation and prognosis of the disease.

[The use of immunoenzyme assay for the control of specific activity of antisera against vasopressin]. / Primenenie immunofermentnogo analiza dlia kontrolia spetsificheskoi aktivnosti antisyvorotok k vazopressinu.

A procedure is described for production of antiserum against peptide hormone Arg-vasopressin. A sensitive immunoenzyme assay is developed for estimation of the antiserum activity and specificity.

[In vitro study of the sensitizing activity of an antirabies immunoglobulin and its fractions]. / Izuchenie in vitro sensibiliziruiushchei aktivnosti antirabicheskogo immunoglobulina i ego fraktsii.

The dynamics of the blood in guinea pigs sensitized with heterogenous antirabic immunoglobulin was studied. The damage of neutrophils in the blood was found to be an index which allowed to detect changed sensitivity to heterogenous protein already at an early period (on day 6) after immunization, whereas the indirect mast cell degranulation test detected it only at a later period (on day 21). The above methods revealed that beta 1 and beta 2 globulin fractions possessed high sensitizing activity.