A comparison of signal sequence prediction methods using a test set of signal peptides.

We describe the creation of a test set containing secretory and non-secretory proteins. Five existing prediction programs for signal sequences and their cleavage sites are compared on the basis of this test set: SPScan, SigCleave, SignalP V1.1, SignalP V2.0. b2-HMM and SignalP V2.0.b2-NN.