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Hum Pathol ; 31(3): 394-5, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10746685

ABSTRACT

To investigate the presence and the role of polyomaviruses JC (JCV), BK (BKV), and the simian polyomavirus (SV40) in human brain tumors, samples from 25 glial-derived tumors (10 astrocytomas, 5 ependymomas, 5 oligodendrogliomas, and 5 glioblastomas) were examined by means of molecular biology and immunohistochemistry. Nested PCR of the large T (LT) region and its sequence analysis showed JCV in 6 cases (4 astrocytomas, 1 oligodendroglioma, and 1 ependymoma), while the transcriptional control region (TCR) was amplified only in 1 astrocytoma, the oligodendroglioma, and the ependymoma, one of which (astrocytoma) also stained positively by immunohistochemistry (JCV LT). TCR sequence analysis of the oligodendroglioma showed a JCV rearranged structure not related to a known viral strain, while the astrocytoma and the ependymoma disclosed a JCV Mad-4 strain that is known to induce brain tumors in animals. We suggest that JCV could have played a role in the pathogenesis of these brain tumors.


Subject(s)
Brain Neoplasms/virology , Glioma/virology , JC Virus/isolation & purification , Adolescent , Adult , Aged , Antigens, Polyomavirus Transforming/analysis , Brain Neoplasms/chemistry , Brain Neoplasms/pathology , DNA, Viral/analysis , Female , Glioma/chemistry , Glioma/pathology , Humans , JC Virus/genetics , Male , Middle Aged , Polymerase Chain Reaction , Simian virus 40/genetics , Simian virus 40/isolation & purification
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