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1.
Eur J Oral Sci ; 108(2): 130-5, 2000 Apr.
Article in English | MEDLINE | ID: mdl-10768726

ABSTRACT

Oxygen reactive intermediates released from phagocytic cells are important for microbicidal activity, but they may also be harmful to surrounding cells and matrix components at the inflammation site. In different forms of inflammatory periodontal disease, peripheral and crevicular polymorphonuclear leukocytes, as well as mononuclear phagocytes and gingival fibroblasts, are exposed to bacterial cell wall components and cytokines. The aim of this study was to evaluate if some bacterial components and cytokines induce superoxide release and superoxide dismutase (SOD) expression in gingival fibroblasts. Lipopolysaccharide (LPS), streptococcal cell walls (SCW), and formyl-methionyl-leucyl-phenylalanine were found to stimulate O2- release from gingival fibroblasts, which increased when Ca2+ was added. Phorbol myristate acetate, a potent activator of respiratory burst in phagocytes, was found to be a weak stimulator of O2- release in gingival fibroblasts. Of the cytokines tested, tumor necrosis factor (TNF)-alpha was found to activate superoxide release in gingival fibroblasts. Gene expression for manganese superoxide dismutase (MnSOD), but not for copper/zinc superoxide dismutase (CuZnSOD), was demonstrated in fibroblasts exposed to LPS, SCW and TNF-alpha using Northern blot analysis. The production of MnSOD may be protective for these cells. We conclude that bacterial cell wall components and cytokines modulate O2- release by gingival fibroblasts which may contribute to periodontal pathology.


Subject(s)
Fibroblasts/enzymology , Free Radical Scavengers/metabolism , Gingiva/enzymology , Superoxide Dismutase/metabolism , Superoxides/metabolism , Analysis of Variance , Blotting, Northern , Calcium/pharmacology , Cell Wall/metabolism , Cells, Cultured , Cytokines/pharmacology , Fibroblasts/cytology , Gene Expression Regulation, Enzymologic , Gingiva/cytology , Humans , Lipopolysaccharides/pharmacology , N-Formylmethionine Leucyl-Phenylalanine/pharmacology , Neutrophils/metabolism , Phagocytes/metabolism , Reactive Oxygen Species/metabolism , Respiratory Burst/drug effects , Streptococcus/metabolism , Superoxide Dismutase/genetics , Tetradecanoylphorbol Acetate/pharmacology , Tumor Necrosis Factor-alpha/pharmacology
2.
J Biol Chem ; 272(48): 30498-503, 1997 Nov 28.
Article in English | MEDLINE | ID: mdl-9374543

ABSTRACT

To study the role of the focal adhesion tyrosine kinase (FAK) in receptor-mediated secretion, we transfected FAK cDNA into a variant (3B6) of the RBL-2H3 mast cell line. This 3B6 cell line expressed low levels of FAK and was defective in high affinity IgE receptor (FcepsilonRI) but not Ca2+ ionophore-mediated secretion. FcepsilonRI-mediated secretion was reconstituted after transfection of wild-type FAK. Histamine release was also enhanced by the stable expression of two mutants of FAK: a kinase-inactive form in which the ATP binding site Lys-454 was replaced by Arg or a mutant in which the autophosphorylation site Tyr-397 was replaced by Phe. Therefore, the catalytic activity and the autophosphorylation site of FAK are not essential for secretion. FcepsilonRI aggregation increased the tyrosine phosphorylation of both mutants of FAK to the same extent as wild-type FAK. Therefore, tyrosine kinases activated by FcepsilonRI aggregation are phosphorylating FAK and some of these phosphorylation sites are other than Tyr-397. These results strongly suggest that FAK plays a role in FcepsilonRI-induced secretion by functioning as an adapter or linker molecule.


Subject(s)
Cell Adhesion Molecules/metabolism , Histamine Release , Mast Cells/physiology , Protein-Tyrosine Kinases/metabolism , Receptors, IgE/physiology , Amino Acid Substitution , Animals , Focal Adhesion Kinase 1 , Focal Adhesion Protein-Tyrosine Kinases , Mast Cells/enzymology , Mice , Phosphorylation , Phosphotyrosine/metabolism , Rats , Receptor Aggregation , Recombinant Proteins , Signal Transduction , Transfection
3.
Cell Signal ; 7(6): 535-44, 1995 Aug.
Article in English | MEDLINE | ID: mdl-8588970

ABSTRACT

Tyrosine phosphorylation of proteins is a mechanism of signalling for different receptors and is important for cell growth and differentiation. Mast cells and basophils are secretory cells that play a role in inflammatory and immediate allergic reactions. The activation/aggregation of different surface receptors on these cells induces tyrosine phosphorylation of proteins. Because these signals are essential for the function of basophils and mast cells, characterizing these pathways could provide methods to specifically regulate the function of these cells. Here we discuss the signals generated by three receptors: the high affinity IgE receptor (Fc epsilon RI) the growth factor receptor, Kit, and integrins.


Subject(s)
Basophils/physiology , Mast Cells/physiology , Receptors, Immunologic/physiology , Signal Transduction/physiology , Tyrosine/metabolism , Animals , Humans , Phosphorylation
4.
J Biol Chem ; 270(20): 12305-9, 1995 May 19.
Article in English | MEDLINE | ID: mdl-7744883

ABSTRACT

The focal adhesion kinase, pp125FAK, is a novel non-receptor protein tyrosine kinase expressed in different cells including mast cells. Here we report that a 77-kDa protein associates with pp125FAK in the mast cell analog, rat basophilic leukemia (RBL-2H3) cells. When pp125FAK immunoprecipitates were subjected to an in vitro kinase assay, there was prominent phosphorylation on tyrosine of pp125FAK and of a 77-kDa protein. By V8 protease digestion mapping and by immunoblotting with two different anti-pp125FAK antibodies, the 77-kDa protein was distinct from pp125FAK. This Fak Associated Protein or FAP was detected in RBL-2H3 cells but not in fibroblasts. The aggregation of the high affinity IgE receptor, Fc epsilon RI, induced the in vivo tyrosine phosphorylation of FAP. However, there was a marked decrease in the in vitro phosphorylation of FAP in the immunoprecipitates from Fc epsilon RI aggregated cells. Both of these Fc epsilon RI-mediated effects were enhanced by cell adhesion. There was strong association of FAP with non-tyrosine-phosphorylated pp125FAK. Thus this interaction does not appear to be mediated by the Src homology 2 domain. Together the data indicate that FAP associates with pp125FAK and suggest that FAP may play a role in Fc epsilon RI signaling.


Subject(s)
Cell Adhesion Molecules/metabolism , Immunoglobulin E/immunology , Immunologic Capping , Mast Cells/metabolism , Protein Processing, Post-Translational , Protein-Tyrosine Kinases/metabolism , Receptors, IgE/immunology , 3T3 Cells/metabolism , Animals , Electrophoresis, Gel, Two-Dimensional , Focal Adhesion Kinase 1 , Focal Adhesion Protein-Tyrosine Kinases , Leukemia, Basophilic, Acute/pathology , Mice , Neoplasm Proteins/metabolism , Phosphorylation , Rats , Tumor Cells, Cultured
5.
J Immunol ; 153(10): 4655-62, 1994 Nov 15.
Article in English | MEDLINE | ID: mdl-7963537

ABSTRACT

Tyrosine phosphorylation of proteins is an essential component of high affinity IgE receptor (Fc epsilon RI) signaling and secretion. This signaling and secretion is also dependent on the organization of the cytoskeleton. Here we report that the aggregation of Fc epsilon RI on rat basophilic leukemia cells results in tyrosine phosphorylation of the cytoskeletal protein, paxillin. Tyrosine phosphorylation of paxillin is a relatively late event after Fc epsilon RI aggregation. Both the direct increase in intracellular Ca2+ with calcium ionophore and the activation of protein kinase C (PKC) with PMA induced tyrosine phosphorylation of paxillin. The optimal tyrosine phosphorylation of paxillin by Fc epsilon RI aggregation required PKC and extracellular Ca2+. However, there was also Fc epsilon RI-mediated tyrosine phosphorylation of paxillin independent of Ca2+ influx or PKC activation. By fluorescent microscopy, cell stimulation induced a redistribution of paxillin toward the periphery of the cells. Although Fc epsilon RI aggregation induced tyrosine phosphorylation of paxillin in nonadherent cells, adherence markedly enhanced this phosphorylation. Together, the data suggest a role for paxillin in Fc epsilon RI signaling.


Subject(s)
Cytoskeletal Proteins/metabolism , Phosphoproteins/metabolism , Protein-Tyrosine Kinases/metabolism , Receptor Aggregation/immunology , Receptors, IgE/immunology , Animals , Blotting, Western , Calcium/metabolism , Cell Adhesion/immunology , Cytoskeletal Proteins/immunology , Microscopy, Confocal , Microscopy, Fluorescence/methods , Paxillin , Phosphoproteins/immunology , Precipitin Tests , Protein Kinase C/metabolism , Rats , Tumor Cells, Cultured
6.
Immunol Today ; 15(2): 62-6, 1994 Feb.
Article in English | MEDLINE | ID: mdl-8155264

ABSTRACT

Basophils and mast cells play a role both in immediate allergic reactions and in inflammation. Both types of cells have surface adhesion receptors that can mediate binding to other cells and to extracellular matrix glycoproteins. Here Majed Hamawy and colleagues discuss the importance of these adhesion molecules in regulating basophil and mast-cell functions.


Subject(s)
Basophils/immunology , Cell Adhesion Molecules/physiology , Mast Cells/immunology , Animals , Cell Division , Cell Line , Cell Movement , Humans , Signal Transduction/immunology
7.
J Periodontol ; 64(5 Suppl): 492-6, 1993 May.
Article in English | MEDLINE | ID: mdl-7686221

ABSTRACT

To determine how the microenvironment in which mast cells are located may influence their function, we explored the effects of fibronectin and fibroblasts on histamine secretion in vitro from a mast cell model, the rat basophilic leukemia (RBL-2H3) cell line. RBL-2H3 cells bound specifically to fibronectin-coated surfaces. Binding was maximal by 1 hour, was not detectable at 0 degrees C or in the absence of Ca++, and was inhibited by preincubating the cells with a synthetic peptide containing the RGD sequence. Adherence to fibronectin stimulated RBL-2H3 cell spreading with a concomitant reorganization of the cytoskeleton and a repositioning of the cytoplasmic granules to the cell periphery. Although adherence to fibronectin did not by itself induce histamine release, when stimulated by either immunologic or non-immunologic means, fibronectin-adherent cells released dramatically more histamine than cells plated in wells coated with BSA only. Thus, RBL-2H3 cells bind specifically to fibronectin, and in so doing are stimulated to undergo changes in morphology and enhanced responsiveness to secretory stimuli. RBL-2H3 cells grown in coculture with 3T3 fibroblasts, but not RBL-2H3 cells grown alone, became responsive to the polymeric synthetic secretagogue Compound 48/80 and the neuropeptide Substance P. Maximum sensitivity to Compound 48/80 was attained by the second week in coculture. Histamine release was dose-dependent, noncytotoxic and occurred even in the absence of extracellular Ca++. Contact between the 2 cell types appeared to be a critical factor. RBL-2H3 cells, separated from 3T3 cells by a 0.45 micron filter, failed to secrete histamine in response to Compound 48/80.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Fibroblasts/physiology , Fibronectins/physiology , Histamine Release/physiology , Leukemia, Basophilic, Acute/pathology , Mast Cells/physiology , Animals , Rats , Tumor Cells, Cultured
8.
J Periodontol ; 64(5 Suppl): 461-6, 1993 May.
Article in English | MEDLINE | ID: mdl-8315569

ABSTRACT

Apoptosis or programmed cell death is a physiological form of cell suicide that is profoundly influenced by the extracellular microenvironment. Apoptosis is characterized by a cascade of genetic and biochemical events that cause cell shrinkage, condensation of cytoplasmic and nuclear material, cleavage of chromosomal DNA into oligonucleosomesized (approximately 200 bp) fragments, and enhanced recognition of the dying cell by phagocytes. Apoptosis differs fundamentally from necrosis, the pathological form of cell death, in which the cell swells and lyses. The capacity to selectively induce apoptosis in leukocytes might be an important physiological mechanism for controlling accumulation of these cells in inflammatory lesions. In this paper, we review our data on apoptosis in human monocytes, cells which contribute both to the persistence and resolution of chronic inflammation. Apoptosis can be initiated when monocytes are cultured in the absence of appropriate exogenous stimulation. For example, addition of chemotactic factors is insufficient to block apoptosis. However, apoptosis in monocytes can be inhibited by adherence in the presence of serum, by microbial products such as lipopolysaccharide, or by certain pro-inflammatory cytokines, such as interleukin 1 and tumor necrosis factor-alpha. Cytokines derived from type 1 helper T cells (e.g., interferon-gamma) inhibit apoptosis whereas those derived from type 2 helper T cells (e.g., interleukin-4) enhance apoptosis in activated monocytes. Thus, cytokines derived from monocytes as well as T cells modulate apoptosis, implicating both autocrine and paracrine regulatory circuits in monocyte survival. The capacity to therapeutically regulate monocyte apoptosis promises to have tremendous value in promoting rapid healing or reducing the immunopathogenesis of chronic inflammation.


Subject(s)
Apoptosis/physiology , Monocytes/physiology , Cell Survival/physiology , Chronic Disease , Cytokines/physiology , Humans , Inflammation
9.
J Biol Chem ; 268(10): 6851-4, 1993 Apr 05.
Article in English | MEDLINE | ID: mdl-8463210

ABSTRACT

The aggregation of the high affinity IgE receptor (Fc epsilon RI) in adherent rat basophilic leukemia (RBL-2H3) cells induces the tyrosine phosphorylation of several proteins. We examined whether focal adhesion-associated tyrosine kinase, pp125FAK, is one of these proteins. Anti-pp125FAK monoclonal antibody immunoblotted and precipitated a 115-kDa tyrosine-phosphorylated protein. In the absence of Fc epsilon RI aggregation, pp125FAK was tyrosine-phosphorylated only in adherent cells. Aggregating Fc epsilon RI in adherent cells markedly enhanced tyrosine phosphorylation of pp125FAK. This increase was detectable within 1 min of Fc epsilon RI aggregation and was maximal by 15 min. In contrast, in nonadherent cells Fc epsilon RI aggregation did not induce tyrosine phosphorylation of pp125FAK. The enhanced influx of calcium by calcium ionophore or the activation of protein kinase C by phorbol myristate acetate induced tyrosine phosphorylation of pp125FAK only in adherent cells. Thus, Fc epsilon RI-induced tyrosine phosphorylation of pp125FAK could be mediated by the activation of protein kinase C and/or the induction of calcium influx. The data indicate that cell adherence is essential for Fc epsilon RI-induced tyrosine phosphorylation of pp125FAK.


Subject(s)
Cell Adhesion Molecules/metabolism , Cell Adhesion , Protein-Tyrosine Kinases/metabolism , Receptor Aggregation , Receptors, IgE/metabolism , Tyrosine/metabolism , Animals , Calcimycin/pharmacology , Focal Adhesion Kinase 1 , Focal Adhesion Protein-Tyrosine Kinases , Immunoblotting , Kinetics , Phosphorylation , Rats , Tetradecanoylphorbol Acetate/pharmacology , Tumor Cells, Cultured
10.
J Biol Chem ; 268(7): 5227-33, 1993 Mar 05.
Article in English | MEDLINE | ID: mdl-8444898

ABSTRACT

Adherence of cells to extracellular matrix components modulates cellular responses. Here we compared the array of tyrosine phosphorylated proteins induced by the aggregation of the high affinity receptor for IgE (Fc epsilon RI) in fibronectin-adherent and in nonadherent rat basophilic leukemia (RBL-2H3) cells. Adherence to fibronectin in the absence of Fc epsilon RI aggregation induced tyrosine phosphorylation of 105-115-kDa proteins. This phosphorylation was reversed by EDTA and by a synthetic peptide containing the sequence Arg-Gly-Asp, demonstrating a requirement for fibronectin-integrin interaction. Aggregation of Fc epsilon RI in fibronectin-adherent cells markedly enhanced the tyrosine phosphorylation of the same 105-115-kDa proteins. There were minimal differences in tyrosine phosphorylation of other proteins induced by the aggregation of Fc epsilon RI in nonadherent and in fibronectin-adherent cells. Direct activation of protein kinase C and/or increase in calcium influx induced the phosphorylation of the 105-115-kDa proteins only in fibronectin-adherent cells. The magnitude of the phosphorylation of the 105-115-kDa proteins induced by the aggregation of Fc epsilon RI in fibronectin-adherent cells was substantially greater than the sum of that due to adherence to fibronectin and the aggregation of Fc epsilon RI in nonadherent cells. Therefore, cell adherence and the aggregation of Fc epsilon RI synergistically regulate tyrosine phosphorylation of the 105-115 kDa proteins.


Subject(s)
Fibronectins/metabolism , Proteins/metabolism , Receptor Aggregation , Receptors, IgE/metabolism , Tyrosine/metabolism , Amino Acid Sequence , Animals , Blotting, Western , Calcimycin/pharmacology , Molecular Sequence Data , Molecular Weight , Phosphorylation , Precipitin Tests , Proteins/chemistry , Rats , Tetradecanoylphorbol Acetate/pharmacology , Tumor Cells, Cultured
11.
J Immunol ; 150(2): 617-24, 1993 Jan 15.
Article in English | MEDLINE | ID: mdl-7678278

ABSTRACT

2H3 subline of rat basophilic leukemia (RBL-2H3) cells are mast cell analogs that lack responsiveness to nonimmunologic stimuli such as compound 48/80 and substance P. To determine if fibroblasts can influence this responsiveness, RBL-2H3 cells were cocultured with confluent monolayers of mouse 3T3 fibroblasts and assayed for secretagogue-induced histamine release. After 1 wk in coculture, RBL-2H3 cells began to respond to compound 48/80. Responsiveness reached a maximum at 2 wk in coculture and remained at this level for an additional 2 wk. Histamine release was specific, noncytotoxic, dose-dependent, and occurred even in the absence of extracellular Ca2+. No soluble factor from 3T3 cells was found that induced these alterations. Moreover, neither recombinant rat or mouse steel factor, at concentrations up to 250 ng/ml, was able to alter RBL-2H3 cell reactivity to compound 48/80. By 2 wk in coculture, RBL-2H3 cells also became responsive to substance P, although no changes in histamine content, Alcian blue+/safranin- staining or type of serine protease were detected. These results show that 3T3 fibroblasts cause an alteration in the functional repertoire of RBL-2H3 cells and that soluble steel factor cannot duplicate the effect.


Subject(s)
Cell Communication , Fibroblasts/physiology , Leukemia, Basophilic, Acute/metabolism , p-Methoxy-N-methylphenethylamine/pharmacology , 3T3 Cells , Animals , Hematopoietic Cell Growth Factors/pharmacology , Histamine/analysis , Histamine Release/drug effects , Leukemia, Basophilic, Acute/pathology , Mice , Phenotype , Rats , Stem Cell Factor , Tumor Cells, Cultured
12.
J Immunol ; 149(3): 862-70, 1992 Aug 01.
Article in English | MEDLINE | ID: mdl-1634775

ABSTRACT

Previously we reported that the mAb AD1 recognized a heavily glycosylated 50- to 60-kDa protein (AD1 Ag) sterically close to the high-affinity IgE receptor on rat basophilic leukemia (RBL-2H3) cells. The N-terminal amino acid sequence of the AD1 Ag was nearly identical to that of human CD63 (melanoma-associated Ag ME491). In this study we cloned the cDNA of AD1 Ag from a rat basophilic leukemia 2H3 cDNA library. An open reading frame of 238 amino acids was identified that contained the N-terminal 43 amino acid sequence. No evidence of a signal peptide was found. However, four predominantly hydrophobic stretches of sequence were predicted to form membrane-spanning helices, and three putative N-glycosylation sites were identified. The AD1 Ag and CD63 were highly conserved between rat and human, suggesting that the sequence of this protein is important for its function. By immunostaining various rat tissues, the AD1 Ag was found localized to mast cells. However, it was located to lysosomes, secretory granules and the plasma membrane of RBL-2H3 cells and to lysosomes and plasma membrane of many other cultured cell lines. The AD1 Ag could be induced by placing cells in culture. Fibroblasts and hepatocytes freshly isolated from rat embryos stained very weakly for AD1 Ag; however, after 24 to 48 h in culture they were strongly positive. This increase in the expression of the AD1 Ag was accompanied by an increase in detectable RNA message. Therefore, AD1/ME491/CD63 Ag is a mast cell marker in tissue, but is also associated with other cells in culture.


Subject(s)
Antigens, CD/genetics , Antigens, Surface/genetics , Mast Cells/physiology , Membrane Glycoproteins/genetics , Platelet Membrane Glycoproteins/genetics , Amino Acid Sequence , Animals , Antigens, CD/metabolism , Base Sequence , Cloning, Molecular , DNA/genetics , Fluorescent Antibody Technique , Gene Expression , Immunohistochemistry , In Vitro Techniques , Leukemia, Experimental , Molecular Sequence Data , Oligodeoxyribonucleotides/chemistry , Platelet Membrane Glycoproteins/metabolism , Polymerase Chain Reaction , RNA, Messenger/genetics , Rats , Sequence Alignment , Tetraspanin 30 , Tissue Distribution , Tumor Cells, Cultured
13.
J Neuroimmunol ; 39(1-2): 163-73, 1992 Jul.
Article in English | MEDLINE | ID: mdl-1320057

ABSTRACT

Recent evidence indicates that astrocytes have a wide range of functions, usually attributed to cells of the immune system, which are critical for maintaining a balanced homeostatic environment in the central nervous system (CNS). Moreover, these cells are known to participate in inflammatory events within the CNS by secreting cytokines such as transforming growth factor-beta (TGF-beta). In this study we have investigated the ability of TGF-beta to influence astrocyte functions. TGF-beta 1 mRNA is constitutively expressed by astrocytes in vitro, and when cultures are stimulated with exogenous TGF-beta 1 an increase in the expression of this mRNA can be shown, suggesting both autocrine and paracrine regulation. In in vitro assays, TGF-beta 1 is chemotactic for astrocytes in a dose-dependent fashion and inhibits astrocyte proliferation. These results indicating signal transduction by TGF-beta 1-prompted studies to explore receptor-ligand interactions on isolated astrocyte populations. In a receptor binding assay, we demonstrate that astrocytes appear to express three distinct TGF-beta receptor subtypes with nearly 10,000 receptors per cell. Thus, TGF-beta may play an important role in regulating astrocyte functions pivotal to the evolution of intracerebral immune responses including recruitment and activation of glial cells at local inflammatory sites within the CNS.


Subject(s)
Astrocytes/physiology , Transforming Growth Factor beta/physiology , Animals , Astrocytes/drug effects , Astrocytes/metabolism , Chemotactic Factors/physiology , Interleukin-1/pharmacology , RNA, Messenger/metabolism , Receptors, Cell Surface/metabolism , Transforming Growth Factor beta/genetics , Transforming Growth Factor beta/pharmacology
14.
J Immunol ; 149(2): 615-21, 1992 Jul 15.
Article in English | MEDLINE | ID: mdl-1378072

ABSTRACT

Rat basophilic leukemia (RBL-2H3) cells are a useful in vitro model for studies of mast cells and basophils. We examined the adherence of RBL-2H3 cells to different extracellular matrix proteins and the effect of such attachment on secretion. The cells bound to fibronectin-coated surfaces with maximum binding by 1 h at 37 degrees C. There was less attachment to laminin, collagen type I, and collagen type IV. There was no adherence to uncoated surfaces or in the absence of Ca2+. Binding to fibronectin was blocked by a synthetic peptide containing the sequence Arg-Gly-Asp. Therefore, the binding of RBL-2H3 cells to fibronectin may be mediated by surface molecules that belong to the integrin family. Adherence to fibronectin-coated surfaces resulted in cell spreading, a reorganization of the cytoskeletal elements, and a redistribution of the secretory granules. Attachment to fibronectin also dramatically enhanced high affinity IgE receptor-mediated histamine release. This enhancement was maximum by 1 h of adherence and lasted for at least 6 h. There was also enhanced secretion by the Ca2+ ionophore A23187. Thus, adherence to fibronectin can enhance both receptor and non-receptor-mediated release. Addition of soluble fibronectin to RBL-2H3 cells in suspension had no effect on secretion. Therefore, enhanced histamine release required cell attachment to immobilized fibronectin. These results suggest that secretion from mast cells/basophils may be modulated by their interaction with the extracellular matrix.


Subject(s)
Basophils/metabolism , Fibronectins/physiology , Mast Cells/metabolism , Animals , Antibodies, Monoclonal/immunology , Antigens, Differentiation, B-Lymphocyte/physiology , Calcimycin/pharmacology , Cell Adhesion , Histamine Release , Oligopeptides/pharmacology , Rats , Receptors, Fc/physiology , Receptors, IgE , Tumor Cells, Cultured
15.
Infect Immun ; 60(4): 1684-6, 1992 Apr.
Article in English | MEDLINE | ID: mdl-1548091

ABSTRACT

Mononuclear phagocytes are essential for adjuvant activity and polyclonal immunoglobulin synthesis induced by endotoxin-associated protein (EP) from Salmonella spp. To define the mechanisms of EP-mediated immunostimulation, we evaluated monocyte functions central to adjuvanticity following exposure to Salmonella typhimurium EP. In this study, we show that EP promotes the survival of monocytes by blocking programmed cell death (apoptosis), enhancing the production of the immunostimulatory cytokine interleukin-1 (IL-1) and stimulating the increased expression of HLA-DR and IL-2 receptors, which are cell membrane proteins that facilitate antigen presentation and IL-2 regulation, respectively. These results indicate that, like lipopolysaccharide, EP is a potent activator of human monocytes and suggest that EP-induced immunostimulation may be mediated, in part, by enhanced monocyte survival, cytokine release, and receptor expression.


Subject(s)
Bacterial Proteins/immunology , Cell Death/drug effects , Endotoxins/immunology , Lipid A/immunology , Monocytes/drug effects , Monocytes/immunology , Salmonella typhimurium/immunology , Dose-Response Relationship, Immunologic , HLA-DR Antigens/biosynthesis , Humans , In Vitro Techniques , Interleukin-1/biosynthesis , Receptors, Interleukin-2/biosynthesis , Up-Regulation
16.
Growth Factors ; 7(1): 73-83, 1992.
Article in English | MEDLINE | ID: mdl-1323980

ABSTRACT

Intraperitoneal injection of Group A streptococcal cell wall (SCW) fragments into female Lewis rats results in the induction of an acute hepatic inflammation that progresses to granulomatous lesions. Kupffer cells have been shown to rapidly clear circulating SCW which triggers production of TGF-beta. In this study, we examined Kupffer cells for the expression of TGF-beta receptors to determine if these cells might be modulated in an autocrine/paracrine fashion by TGF-beta during SCW-hepatic inflammation. By receptor crosslinking and subsequent SDS-PAGE analysis we demonstrate that Kupffer cells express Type I TGF-beta receptors, but not Types II and III. Scatchard analysis indicated a receptor density of approximately 1100 receptors per cell. Functionally, TGF-beta was found to be chemotactic for Kupffer cells in vitro and this chemotactic response was higher in cells isolated from rats 1-21 days post SCW-injection. Although TGF-beta 1 mRNA is constitutively expressed by Kupffer cells, in vitro stimulation of the cultures with purified TGF-beta augments the expression of TGF-beta 1 mRNA and protein synthesis suggesting autocrine/paracrine regulation. These results indicate that TGF beta secreted by Kupffer cells during SCW-induced hepatic inflammation may amplify its own expression and regulate Kupffer cell functions relevant to the formation of granulomatous lesions within the liver.


Subject(s)
Granuloma/physiopathology , Kupffer Cells/physiology , Liver Diseases/physiopathology , Receptors, Cell Surface/metabolism , Streptococcal Infections/physiopathology , Transforming Growth Factor beta/genetics , Transforming Growth Factor beta/pharmacology , Animals , Cell Wall , Cells, Cultured , Chemotaxis/drug effects , DNA Probes , Granuloma/pathology , Kupffer Cells/drug effects , Kupffer Cells/pathology , Liver Diseases/pathology , Male , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Rats, Inbred Lew , Receptors, Cell Surface/isolation & purification , Receptors, Transforming Growth Factor beta , Streptococcal Infections/pathology , Streptococcus pyogenes , Transforming Growth Factor beta/metabolism
18.
J Immunol ; 147(8): 2559-64, 1991 Oct 15.
Article in English | MEDLINE | ID: mdl-1655894

ABSTRACT

Peritoneal and peripheral blood monocyte-macrophages from inbred Lewis (LEW) rats generate higher levels of reactive oxygen intermediates (ROI) in response to group A streptococcal cell walls (SCW) than do similar populations of cells from histocompatible Fischer rats. This differential sensitivity of the phagocytes to SCW is reflected in differences in susceptibility of the two strains to the development of arthritis in response to SCW. After systemic administration of the SCW, LEW rats develop acute and chronic erosive polyarthritis, whereas the Fischer rats are arthritis resistant. Inasmuch as these data suggested that the SCW-induced release of inflammatory cell products such as ROI might be an important contributory factor in the pathogenesis of arthritis in the LEW rats, the animals were injected with SCW and treated with ROI inhibitors. A single intraarticular injection of superoxide dismutase or catalase significantly reduced the SCW-induced inflammatory response and evolution of erosive arthritis in the treated animals (articular index 3.6 +/- 0.36 for SCW only vs 1.4 +/- 0.3 for SCW + SOD; p less than 0.001; n = 6). These data indicate that ROI play a pivotal role in synovitis and, furthermore, that suppression of these inflammatory mediators modulates both acute and chronic SCW-induced inflammation of the joint.


Subject(s)
Arthritis/etiology , Oxygen/metabolism , Animals , Arthritis/immunology , Arthritis/prevention & control , Catalase/pharmacology , Cell Wall/immunology , Female , Free Radicals , Rats , Rats, Inbred F344 , Rats, Inbred Lew , Streptococcus pyogenes/immunology , Superoxide Dismutase/pharmacology , Superoxides/metabolism
19.
J Exp Med ; 173(4): 981-91, 1991 Apr 01.
Article in English | MEDLINE | ID: mdl-2007861

ABSTRACT

The multifunctional cytokine, transforming growth factor beta (TGF-beta), was identified by immunocytochemistry in the brain tissues of four patients with acquired immune deficiency syndrome (AIDS), but not in control brain tissue. The TGF-beta staining was localized to cells of monocytic lineage as well as astrocytes, especially in areas of brain pathology. In addition, the brain tissues from the AIDS patients contained transcripts for human immunodeficiency virus 1 (HIV-1) by in situ hybridization, suggesting a correlation between the presence of HIV-1 in the brain and the expression of TGF-beta. However, the expression of TGF-beta was not limited to HIV-1-positive cells, raising the possibility of alternative mechanisms for the induction of TGF-beta in these AIDS patients' brains. To investigate these mechanisms, purified human monocytes were infected in vitro with HIV-1 and were shown to secrete increased levels of TGF-beta. In addition, HIV-1-infected monocytes released a factor(s) capable of triggering cultured astrocytes that are not infected with HIV-1 to secrete TGF-beta. The release of TGF-beta, which is an extremely potent chemotactic factor, may contribute to the recruitment of HIV-1-infected monocytic cells, enabling viral spread to and within the central nervous system (CNS). Moreover, TGF-beta augments cytokine production, including cytokines known to be neurotoxic. The identification of TGF-beta within the CNS implicates this cytokine in the immunopathologic processes responsible for AIDS-related CNS dysfunction.


Subject(s)
AIDS Dementia Complex/physiopathology , Astrocytes/physiology , Macrophages/physiology , Transforming Growth Factor beta/physiology , Astrocytes/metabolism , Blotting, Northern , Brain/microbiology , HIV-1/growth & development , Humans , In Vitro Techniques , Male , Monocytes/metabolism , RNA, Messenger/genetics , Transforming Growth Factor beta/genetics
20.
J Immunol ; 144(9): 3518-22, 1990 May 01.
Article in English | MEDLINE | ID: mdl-2158512

ABSTRACT

Previous studies from our laboratory have demonstrated that exposure of human monocytes to a stimulant, such as Con A, results in the production of the enzyme collagenase through PGE2-dependent pathway. Inasmuch as rIFN-gamma has been shown to modulate monocyte/macrophage PG synthesis, we examined the effect of rIFN-gamma on the activation sequence leading to collagenase production. The addition of rIFN-gamma (10 to 1000 U/ml) to Con A-stimulated monocytes resulted in a dose-dependent inhibition of PGE2 and collagenase synthesis. The suppression of collagenase production by rIFN-gamma was related to its ability to reduce PGE2 levels as demonstrated by the restoration of collagenase activity by the addition of PGE2. HPLC analysis of the arachidonic acid (AA) metabolites released by monocytes showed that rIFN-gamma caused a reduction in the release of AA and products of the cyclooxygenase and lipoxygenase pathways. These data indicated that rIFN-gamma decreased eicosanoid production by inhibiting the release of AA from phospholipids. This conclusion was supported by the reduction in membrane bound phospholipase activity in rIFN-gamma-treated monocytes. Moreover, the inhibition by rIFN-gamma of PGE2 and collagenase was reversed by the addition of phospholipase A2. Our findings demonstrate that rIFN-gamma inhibits phospholipase activity in activated monocytes and as a result blocks PGE2-dependent collagenase synthesis.


Subject(s)
Dinoprostone/biosynthesis , Interferon-gamma/pharmacology , Microbial Collagenase/biosynthesis , Monocytes/enzymology , Phospholipases/antagonists & inhibitors , Cells, Cultured , Dinoprostone/pharmacology , Humans , In Vitro Techniques , Lipoxygenase/metabolism , Phospholipases A/metabolism , Phospholipases A2 , Prostaglandin-Endoperoxide Synthases/metabolism , Recombinant Proteins
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