Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
1.
Lipids Health Dis ; 17(1): 285, 2018 Dec 13.
Article in English | MEDLINE | ID: mdl-30545366

ABSTRACT

BACKGROUND: The focus of studies on high-density lipoproteins (HDL) has shifted from HDL-cholesterol (HDL-C) to HDL function. We recently demonstrated that low USF1 expression in mice and humans associates with high plasma HDL-C and low triglyceride levels, as well as protection against obesity, insulin resistance, and atherosclerosis. Here, we studied the impact of USF1 deficiency on HDL functional capacity and macrophage atherogenic functions, including inflammation, cholesterol efflux, and cholesterol accumulation. METHODS: We used a congenic Usf1 deficient mice in C57Bl/6JRccHsd background and blood samples were collected to isolate HDL for structural and functional studies. Lentiviral preparations containing the USF1 silencing shRNA expression vector were used to silence USF1 in human THP-1 and Huh-7 cells. Cholesterol efflux from acetyl-LDL loaded THP-1 macrophages was measured using HDL and plasma as acceptors. Gene expression analysis from USF1 silenced peritoneal macrophages was carried out using Affymetrix protocols. RESULTS: We show that Usf1 deficiency not only increases HDL-C levels in vivo, consistent with elevated ABCA1 protein expression in hepatic cell lines, but also improves the functional capacity of HDL particles. HDL particles derived from Usf1 deficient mice remove cholesterol more efficiently from macrophages, attributed to their higher contents of phospholipids. Furthermore, silencing of USF1 in macrophages enhanced the cholesterol efflux capacity of these cells. These findings are consistent with reduced inflammatory burden of USF1 deficient macrophages, manifested by reduced secretion of pro-inflammatory cytokines MCP-1 and IL-1ß and protection against inflammation-induced macrophage cholesterol accumulation in a cell-autonomous manner. CONCLUSIONS: Our findings identify USF1 as a novel factor regulating HDL functionality, showing that USF1 inactivation boosts cholesterol efflux, reduces macrophage inflammation and attenuates macrophage cholesterol accumulation, linking improved macrophage cholesterol metabolism and inflammatory pathways to the antiatherogenic function of USF1 deficiency.


Subject(s)
Cholesterol, HDL/genetics , Cholesterol/genetics , Lipoproteins, HDL/genetics , Upstream Stimulatory Factors/genetics , ATP Binding Cassette Transporter 1/genetics , Animals , Chemokine CCL2/genetics , Cholesterol/blood , Gene Expression/genetics , Humans , Inflammation/blood , Inflammation/genetics , Inflammation/pathology , Insulin Resistance/genetics , Lipoproteins, HDL/blood , Macrophages, Peritoneal/metabolism , Macrophages, Peritoneal/pathology , Male , Mice , Mice, Knockout , Obesity/blood , Obesity/genetics , Obesity/pathology
2.
Sci Transl Med ; 8(323): 323ra13, 2016 Jan 27.
Article in English | MEDLINE | ID: mdl-26819196

ABSTRACT

USF1 (upstream stimulatory factor 1) is a transcription factor associated with familial combined hyperlipidemia and coronary artery disease in humans. However, whether USF1 is beneficial or detrimental to cardiometabolic health has not been addressed. By inactivating USF1 in mice, we demonstrate protection against diet-induced dyslipidemia, obesity, insulin resistance, hepatic steatosis, and atherosclerosis. The favorable plasma lipid profile, including increased high-density lipoprotein cholesterol and decreased triglycerides, was coupled with increased energy expenditure due to activation of brown adipose tissue (BAT). Usf1 inactivation directs triglycerides from the circulation to BAT for combustion via a lipoprotein lipase-dependent mechanism, thus enhancing plasma triglyceride clearance. Mice lacking Usf1 displayed increased BAT-facilitated, diet-induced thermogenesis with up-regulation of mitochondrial respiratory chain complexes, as well as increased BAT activity even at thermoneutrality and after BAT sympathectomy. A direct effect of USF1 on BAT activation was demonstrated by an amplified adrenergic response in brown adipocytes after Usf1 silencing, and by augmented norepinephrine-induced thermogenesis in mice lacking Usf1. In humans, individuals carrying SNP (single-nucleotide polymorphism) alleles that reduced USF1 mRNA expression also displayed a beneficial cardiometabolic profile, featuring improved insulin sensitivity, a favorable lipid profile, and reduced atherosclerosis. Our findings identify a new molecular link between lipid metabolism and energy expenditure, and point to the potential of USF1 as a therapeutic target for cardiometabolic disease.


Subject(s)
Adipose Tissue, Brown/metabolism , Upstream Stimulatory Factors/deficiency , Upstream Stimulatory Factors/genetics , Adult , Aged , Alleles , Animals , Atherosclerosis/metabolism , Blood Glucose/metabolism , Carbohydrates/chemistry , Cardiovascular System , Cholesterol, HDL/blood , Cholesterol, HDL/metabolism , Cohort Studies , Female , Gene Silencing , Glucose/metabolism , Humans , Insulin/blood , Insulin/metabolism , Lipids/chemistry , Lipoprotein Lipase/metabolism , Lipoproteins, VLDL/metabolism , Liver/metabolism , Male , Metabolic Syndrome/genetics , Mice , Mice, Inbred C57BL , Mice, Transgenic , Middle Aged , Oxygen Consumption , Phenotype , Polymorphism, Single Nucleotide , Thermogenesis , Triglycerides/blood , Triglycerides/metabolism
3.
J Mol Med (Berl) ; 87(8): 825-35, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19554302

ABSTRACT

Analysis of variants in three genes encoding oxysterol-binding protein (OSBP) homologues (OSBPL2, OSBPL9, OSBPL10) in Finnish families with familial low high-density lipoprotein (HDL) levels (N = 426) or familial combined hyperlipidemia (N = 684) revealed suggestive linkage of OSBPL10 single-nucleotide polymorphisms (SNPs) with extreme end high triglyceride (TG; >90th percentile) trait. Prompted by this initial finding, we carried out association analysis in a metabolic syndrome subcohort (Genmets) of Health2000 examination survey (N = 2,138), revealing association of multiple OSBPL10 SNPs with high serum TG levels (>95th percentile). To investigate whether OSBPL10 could be the gene underlying the observed linkage and association, we carried out functional experiments in the human hepatoma cell line Huh7. Silencing of OSBPL10 increased the incorporation of [(3)H]acetate into cholesterol and both [(3)H]acetate and [(3)H]oleate into triglycerides and enhanced the accumulation of secreted apolipoprotein B100 in growth medium, suggesting that the encoded protein ORP10 suppresses hepatic lipogenesis and very-low-density lipoprotein production. ORP10 was shown to associate dynamically with microtubules, consistent with its involvement in intracellular transport or organelle positioning. The data introduces OSBPL10 as a gene whose variation may contribute to high triglyceride levels in dyslipidemic Finnish subjects and provides evidence for ORP10 as a regulator of cellular lipid metabolism.


Subject(s)
Cholesterol, HDL/blood , Hyperlipidemia, Familial Combined/genetics , Lipid Metabolism , Polymorphism, Single Nucleotide , Receptors, Steroid/genetics , Triglycerides/blood , Cell Line, Tumor , Cholesterol, HDL/genetics , Cholesterol, HDL/metabolism , Female , Finland , Gene Silencing , Hepatocytes/metabolism , Humans , Hyperlipidemia, Familial Combined/metabolism , Male , Microtubules/chemistry , Receptors, Steroid/analysis , Receptors, Steroid/metabolism , Triglycerides/genetics , Triglycerides/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...