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1.
Curr Microbiol ; 76(4): 485-494, 2019 Apr.
Article in English | MEDLINE | ID: mdl-30783798

ABSTRACT

Burkholderia contaminans is a member of the Burkholderia cepacia complex (Bcc), a pathogen with increasing prevalence among cystic fibrosis (CF) patients and the cause of numerous outbreaks due to the use of contaminated commercial products. The antibiotic resistance determinants, particularly ß-lactamases, have been poorly studied in this species. In this work, we explored the whole genome sequence (WGS) of a B. contaminans isolate (FFH 2055) and detected four putative ß-lactamase-encoding genes. In general, these genes have more than 93% identity with ß-lactamase genes found in other Bcc species. Two ß-lactamases, a class A (Pen-like, suggested name PenO) and a class D (OXA-like), were further analyzed and characterized. Amino acid sequence comparison showed that Pen-like has 82% and 67% identity with B. multivorans PenA and B. pseudomallei PenI, respectively, while OXA-like displayed strong homology with class D enzymes within the Bcc, but only 22-44% identity with available structures from the OXA family. PCR reactions designed to study the presence of these two genes revealed a heterogeneous distribution among clinical and industrial B. contaminans isolates. Lastly, blaPenO gene was cloned and expressed into E. coli to investigate the antibiotic resistance profile and confers an extended-spectrum ß-lactamase (ESBL) phenotype. These results provide insight into the presence of ß-lactamases in B. contaminans, suggesting they play a role in antibiotic resistance of these bacteria.


Subject(s)
Bacterial Proteins/genetics , Burkholderia cepacia complex/enzymology , Burkholderia cepacia complex/genetics , Genome, Bacterial/genetics , beta-Lactamases/genetics , Amino Acid Sequence , Anti-Bacterial Agents/pharmacology , Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Burkholderia Infections/microbiology , Burkholderia cepacia complex/drug effects , Cystic Fibrosis/microbiology , Drug Resistance, Multiple, Bacterial , Escherichia coli/drug effects , Escherichia coli/genetics , Humans , Microbial Sensitivity Tests , Models, Molecular , Sequence Homology, Amino Acid , beta-Lactamases/chemistry , beta-Lactamases/metabolism
2.
Genome Announc ; 3(2)2015 Apr 02.
Article in English | MEDLINE | ID: mdl-25838490

ABSTRACT

Empedobacter (formerly Wautersiella) falsenii comb. nov. strain Wf282 was isolated from a cervical neck abscess sample from an 18-year-old female patient. The isolate was resistant to many antibiotics, including meropenem and colistin. The total DNA from the multidrug-resistant E. falsenii comb. nov. Wf282 clinical isolate was sequenced.

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