Synergistic impact of innate immunity hyper-activation and endothelial dysfunction on the magnitude of organ failure in the infection-sepsis continuum.

OBJECTIVES: Identifying host response biomarkers implicated in the emergence of organ failure during infection is key to improving the early detection of this complication. METHODS: Twenty biomarkers of innate immunity, T-cell response, endothelial dysfunction, coagulation, and immunosuppression were profiled in 180 surgical patients with infections of diverse severity (IDS) and 53 with no infection (nIDS). Those better differentiating IDS/nIDS in the area under the curve were combined to test their association with the sequential organ failure assessment score by linear regression analysis in IDS. Results were validated in another IDS cohort of 174 patients. RESULTS: C-reactive protein, procalcitonin, pentraxin-3, lipocalin-2 (LCN2), tumoral necrosis factor-α, angiopoietin-2, triggering receptor expressed on myeloid cells-1 (TREM-1) and interleukin (IL)-15 yielded an area under the curve ≥0.75 to differentiate IDS from nIDS. The combination of LCN2, IL-15, TREM-1, angiopoietin-2 (Dys-4) showed the strongest association with sequential organ failure assessment score in IDS (adjusted regression coefficient; standard error; P): Dys-4 (3.55;0.44; <0.001), LCN2 (2.24; 0.28; <0.001), angiopoietin-2 (1.92; 0.33; <0.001), IL-15 (1.78; 0.40; <0.001), TREM-1(1.74; 0.46; <0.001), tumoral necrosis factor-α (1.60; 0.31; <0.001), pentraxin-3 (1.12; 0.18; <0.001), procalcitonin (0.85; 0.12; <0.001). Dys-4 provided similar results in the validation cohort. CONCLUSIONS: There is a synergistic impact of innate immunity hyper-activation (LCN2, IL-15, TREM-1) and endothelial dysfunction (angiopoietin-2) on the magnitude of organ failure during infection.

Benzene and Borazine, so Different, yet so Similar: Insight from Experimental Charge Density Analysis.

Although benzene and borazine are isoelectronic and isostructural, they have very different electronic structures, mainly due to the polar nature of the B-N bond. Herein, we present an experimental study of the charge density distribution obtained from the multipole model formalism and Hirshfeld atom refinement (HAR) based on high-resolution X-ray diffraction data of borazine B3N3H6 (1) and B,B',B″-trichloroborazine (2) crystals. These data are compared to those obtained from HAR for benzene (4) and 1,3,5-trichlorobenzene (5) and further compared with values obtained from density functional theory calculations in the gas phase, where N,N',N″-trichloroborazine (3) was also included. The results confirm that, unlike benzene, borazines are only weakly aromatic with an island-like electronic delocalization within the B3N3 ring involving only the nitrogen atoms. Furthermore, delocalization indices and interacting quantum atom energy for bonded and non-bonded atoms were found to be highly suitable indicators capable of describing the origin of the discrepancies observed when the degree of aromaticity in 2 and 3 is evaluated using common aromaticity indices. Additionally, analysis of intermolecular interactions in the crystals brings further evidence of a weakly aromatic character of the borazines as it reveals surprising similarities between the crystal packing of borazine and benzene and also between B,B',B″-trichloroborazine and 1,3,5-trichlorobenzene.

Digital Intimate Partner Violence Among Peruvian Youths: Validation of an Instrument and a Theoretical Proposal.

The present study presents psychometric information on a new instrument, the Digital Intimate Partner Violence Questionnaire (DIPVQ), and explores the similitudes and differences between in-person and digital-based abuses (those that involve the use of information and communication technologies [ICTs]). In all, 449 Peruvian students took part in the study (X = 21.2 years; SD = 4.3 years; 73% women). DIPVQ structure was determined by carrying out an exploratory factor analysis (EFA) with polychoric correlation matrices and oblique rotation. In-person violence was assessed using the Dating Violence Questionnaire (DVQ) and self-labeling questions (e.g., feeling trapped, afraid, and abused). Relationship satisfaction was assessed using the Perceived Relationship Quality Components-Short Form (PRQC-SF). EFA showed a two-scale structure for the DIPVQ: control-centered cyberabuse (N = 5; control, monitoring, and identity theft; EAP alpha = .96) and damage-centered cyberabuse (N = 7; unwanted sexual contents, blackmailing, and causing debts throughout ICT; Expected-A-Posteriori alpha = .97). DIPVQ had direct relationship to DVQ and self-labeling (p < .001; d = 0.38-1.18), and inverse to PRQC-SF (p = .11; d = .22-.33). Behaviors such as impersonation and monitoring were reported by more than 20% of participants. Online and offline victimization coexist in 42% of cases, while 3.6% of aggressions happened exclusively via ICT. DIPVQ is a valid and reliable measure of digital victimization. The control-centered scale had a higher frequency, although the damage-centered scale had stronger relationship to feeling afraid and abused. While previous literature has classified online aggressions regarding their aesthetic appearance, it seems that their functional value (control vs. hurting) could provide a better framework for understanding these aggressions.