ABSTRACT
The medical social characteristics of injured patients hospitalized in the traumatic surgery department of municipal hospital. The study results permitted to develop the organizational functional model of injury prevention management. The pattern presuppose the implementation of complex of activities considering the options of target population groups and measures realization period based on the regular occurrences revealed in the study.
Subject(s)
Health Status Indicators , Inpatients , Trauma Centers/organization & administration , Wounds and Injuries/classification , Female , Humans , Male , Retrospective Studies , Russia , Severity of Illness Index , Wounds and Injuries/prevention & controlABSTRACT
The analysis of the medical social portrait of the deceased in consequence of traffic accidents was implemented. The risk groups and the pattern of mortality indicators distribution according the days of week and seasons were revealed. The major medical social factors of traffic accidents making resulted in fatal outcomes were established considering the characteristics of injuries due to traffic accidents demanding the organization of opportune medical care.
Subject(s)
Accidents, Traffic/mortality , Automobile Driving/statistics & numerical data , Employment/statistics & numerical data , Adolescent , Adult , Female , Health Status Indicators , Humans , Male , Middle Aged , Russia/epidemiology , Socioeconomic Factors , Young AdultABSTRACT
It has been shown, that the total X-ray irradiation in the dozes of 0.5 and 1 Gy influences on the content of lipid peroxidation products and enzymatic activity of antioxidant system in rat spleen and thymus cells. The influence of preparations "AMMIVIT" and "Ceruloplasmin" on these processes is investigated also. So, the animals feeding by the vitamin concentrate "AMMIVIT" have lead to increase of MDA level (a final product of lipid peroxidation) and the overactivity of some antioxidant enzymes in rat spleen and thymus cells. Injection of the preparation "Ceruloplasmin" to experimental animals up 1 hour before the irradiation has normalized LPO intensity and activity of AO enzymes.
Subject(s)
Antioxidants/pharmacology , Ceruloplasmin/pharmacology , Lipid Peroxidation/drug effects , Animals , Antioxidants/metabolism , Dose-Response Relationship, Radiation , Male , Malondialdehyde/metabolism , Rats , Rats, Wistar , Spleen/metabolism , Spleen/radiation effects , Superoxide Dismutase/metabolism , Thymus Gland/metabolism , Thymus Gland/radiation effectsABSTRACT
The squalene synthase (SQS) gene encodes a key regulatory enzyme, farnesyl-diphosphate farnesyltransferase (EC 2.5.1.21), in sterol biosynthesis. The SQS1 gene was isolated from a subgenomic library of the industrially important yeast Yarrowia lipolytica, using PCR-generated probes. Probes were based on conserved regions of homologues from different organisms. The complete nucleotide sequence of the coding region and the corresponding amino acid sequence were determined. The sequences showed extensive homologies with squalene synthase genes and enzymes from a number of other organisms and extreme amino acid conservation within the binding and catalytic domains. Direct cloning of a 4.3 kb genomic Y. lipolytica fragment, also comprising its own promoter and terminator sequences, into autonomously replicating plasmid YEp352 and subsequent transformation of a Saccharomyces cerevisiae mutant strain with relevant erg9: ura3-1 markers, resulted in functional complementation of these deficiencies, although Northern blot analyses did not reveal a unique full-length messenger. The availability of the Y. lipolytica SQS1 gene (GenBank Accession No. AF092497) offers prospects for metabolic engineering of the isoprenoid and sterol biosynthetic pathways.
Subject(s)
Farnesyl-Diphosphate Farnesyltransferase/genetics , Farnesyl-Diphosphate Farnesyltransferase/metabolism , Saccharomyces cerevisiae/enzymology , Saccharomycetales/enzymology , Saccharomycetales/genetics , Amino Acid Sequence , Base Sequence , Blotting, Northern , Cloning, Molecular , Genes, Fungal , Genetic Complementation Test , Molecular Sequence Data , Polymerase Chain Reaction/methods , Saccharomyces cerevisiae/genetics , Sequence Analysis, DNASubject(s)
Radiation Injuries, Experimental/prevention & control , Radiation Tolerance/drug effects , Radiation-Protective Agents/pharmacology , Vitamins/pharmacology , Administration, Oral , Amino Acids/administration & dosage , Amino Acids/pharmacology , Animals , Ascorbic Acid/administration & dosage , Ascorbic Acid/pharmacology , Drug Combinations , Male , Mice , Mice, Inbred BALB C , Radiation Dosage , Radiation-Protective Agents/administration & dosage , Survival Analysis , Vitamin B Complex/administration & dosage , Vitamin B Complex/pharmacology , Vitamins/administration & dosageABSTRACT
The aim of this work was research of the morphofunctional features of rabbit free skin flaps after CO2 laser and scalpel interventions. pO2, histologic and histochemical data were analyzed. The results evidence an insignificant delay of the pO2 growth in laser-treated flaps early after surgery. This fact hints at a low speed of laser-treated flaps vascularization because of wound coagulation necrosis. However, this does not influence the course of the reparative process. Our conclusions are confirmed by histologic and histochemical findings.
Subject(s)
Laser Therapy , Regeneration/physiology , Skin Transplantation/physiology , Skin/physiopathology , Animals , Histocytochemistry , Laser Therapy/methods , Oxygen Consumption/physiology , Partial Pressure , Rabbits , Skin Transplantation/methods , Time Factors , Transplantation, AutologousABSTRACT
In this study in 90 rats the measured rate of healing, pO2 in sites of postoperative scar and histological specimen of identical excisional wounds produced by CO2-laser, scalpel and combination are compared. The data of healing laser wounds via scalpel ones show slow laser wound healing. We explain, this by inhibition of the inflammation phase due to diminishing macrophages migration into the wound. pO2 data in postoperative scar after laser and scalpel wounds show that laser thermal alteration does not influence CO2 regimen in the scar.
