[Structure and biochemical parameters of glycoconjugates of alveolar macrophages exposed to fibrogenic dusts]. / Strukturno-biokhimicheskaia kharakteristika glikokoniugatov alveoliarnykh makrofagov pri deistvii fibrogennykh pylei.

Changes in the metabolism of rat alveolar macrophages exposed to quartz and coal dusts were studied by biochemical methods. The intratracheal administration of quartz and coal dusts in 6 hours induced the augmented storage of glycoproteins on the cell surface and the increased lysosomal enzymes (beta-galactosidase, beta-glucuronidase) activities. The quartz dust also induced the decrease of hexauronic acids level. The interaction of macrophage with the fibrogenic dust (quartz) may cause severe disorders in the balance of carbohydrate constituents of glycocalyx, which impairs the vitality of alveolar macrophages.

The influence of tetrachloromethane on subcellular structures of rat hepatocyte lysosomal and cytoplasmic enzymes of the liver, lungs and blood serum of rats during continuous and intermittent action of tetrachloromethane.

The authors performed a comparative biochemical study of some enzymes of lysosomic origin (hyaluronidase, N-acetyl-beta-D-glucosaminidase, beta-glucosidase, beta-galatosidase and acid phosphatase), of the state of enzyme substrate system N-acetylneuraminic acid---aldolase of neuramic acid and of the activity of lactatedehydrogenase (soluble in cytosol and bound on mitochodria) in the liver, lungs and blood serum of rats at various regimens of the inhalation action of CCl4. On the basis of results obtained they determined the biological importance of the change of activity of enzymes differently localized in cells at the adaptation of an organisme to the noxious action of CCl4.