ABSTRACT
The involvement of upper electron-excited states in bacterial bioluminescence process was studied with excitation energy-accepting molecules. The fluorescent aromatic compounds, anthracene and 1.4-bis(5-phenyloxazol-2-yl)benzene, were chosen. Energies of their lowest excited singlet states are higher than the energy of the analogous state of the bioluminescence emitter; their absorption spectra and bioluminescence do not overlap. Hence, the excitation of these molecules by singlet-singlet energy transfer or by light absorption is excluded. Sensitized fluorescence of these compounds in the bioluminescence systems has been recorded, indicating the activity of upper electron-excited states in the bioluminescent process.
Subject(s)
Electrons , Luminescent Measurements , Photobacterium/enzymology , Anthracenes/chemistry , Energy Transfer , Oxazoles/chemistry , Spectrometry, FluorescenceABSTRACT
The spatial distribution of two-photon excitation created by crossed focused Gaussian beams is shown. The effective volume calculation for the two-photon interaction of crossed Gaussian beams is presented. The dimensions of cubic and parallelepiped volumes, in which half of the two-photon interaction is localized, are determined. The memory densities of two-photon three-dimensional memory for crossed focused beams and one focused beam are compared.
