ABSTRACT
The representation patterns of 15 cytokines RNA in blood plasma and blood cells of patients with breast cancer and apparently healthy women were investigated. Relative levels of RNA IL-8 and IL-18 in plasma of breast cancer patients are significantly increased compared with control group. At the same time no obvious differences were found in relative concentrations of these transcripts in blood cells of patients and control groups. Relative concentration of IL-8 RNA was higher in blood plasma of locally advanced compared with early breast cancer patients.
Subject(s)
Breast Neoplasms/diagnosis , Interleukin-18/biosynthesis , Interleukin-8/biosynthesis , RNA/blood , Adult , Aged , Breast Neoplasms/blood , Breast Neoplasms/pathology , Female , Humans , Middle Aged , Neoplasm Staging , Transcription, GeneticABSTRACT
The impact of various general anesthesia modes on the oxidant-antioxidant system (OAS) and cognitive function in the perioperative period was studied in 88 patients (4 groups, each comprising 22 patients according to the mode of general anesthesia who had undergone elective surgery for chronic calculous cholecystitis (out of an exacerbation) and inguinal, umbilical, and postoperative hernias. Oxidative protein and lipid damages with the lower activity of antioxidant defense enzymes were found in the surgical patients exposed to various modes of general anesthesia. The findings suggest that the pattern of changes in OAS parameters depends on the method of anesthetic maintenance. Oxidative protein modification is much more frequently recorded that lipid peroxidation. Propofol-based balanced anesthesia has a minimal impact on OAS parameters as compared to neuroleptic analgesia, ataralgesia, and sevoflurane anesthesia. After elective abdominal surgery, decreased free radical oxidation under propofol-anesthesia is concurrent with lower postoperative cognitive dysfunction.
Subject(s)
Abdomen/surgery , Anesthesia, General/methods , Anesthetics, Intravenous/adverse effects , Antioxidants/metabolism , Cognition/drug effects , Propofol/adverse effects , Reactive Oxygen Species/metabolism , Elective Surgical Procedures , Humans , Lipid Peroxidation/drug effects , Neuropsychological Tests , Oxidative Stress/drug effects , Reactive Oxygen Species/bloodABSTRACT
Hyperexpression of epidermal growth factor receptor (EGFR) is often identified as unfavorable prognosis for different epithelial cancers. The study was concerned with an attempt of establishing a relationship between EGFR expression, on the one hand, and patient's clinico-morphological status, prognosis and efficacy of chemotherapy for stage III-IV serous ovarian carcinoma, on the other. EGFR hyperexpression predominated in advanced aggressive tumors and involved a significantly shorter period preceding tumor progression. Similarly, overall survival median in patients with EGFR hyperexpression (21+/-4 months) appeared lower than without it (42+/-8 months). In serous ovarian carcinoma stage III-IV, EGFR hyperexpression should be considered sufficient for prognosis of chemotherapy efficacy, pre-progression time and survival.
Subject(s)
ErbB Receptors/analysis , Ovarian Neoplasms/chemistry , Ovarian Neoplasms/pathology , Adult , Aged , Disease-Free Survival , Female , Gene Expression Regulation, Neoplastic , Humans , Middle Aged , Neoplasm Staging , Predictive Value of Tests , Prognosis , Retrospective Studies , Up-RegulationABSTRACT
The spectrum of human antigens allows a monitoring of various pathological processes such as autoimmune disorders and tumorigenesis. Serological analysis of cDNA expression libraries (SEREX) is now used to search for new cancer-associated antigens, which are potential diagnostic markers or targets for immunotherapy of cancer. The results obtained for several solid tumors are reviewed. Groups of antigens detectable by SEREX, causes of immunogenicity of autoantigens, and prospective implication of antigens in diagnostics and monitoring of cancer are discussed.