ABSTRACT
PREMISE OF THE STUDY: We developed and characterized nuclear microsatellite markers for Anadenanthera colubrina, a tropical tree species widely distributed in South America. METHODS AND RESULTS: Leaf samples of mature A. colubrina trees, popularly called "angico," were collected from an area that is greatly impacted by agricultural practices in the region of Ribeirão Preto in São Paulo State in southeastern Brazil. Twenty simple sequence repeat (SSR) markers were developed, 14 of which had polymorphic loci. A total of 96 alleles were detected with an average of 6.86 alleles per polymorphic locus. The expected heterozygosity, calculated at polymorphic loci, ranged from 0.18 to 0.83. Finally, we demonstrated that 18 loci were cross-amplified in A. peregrina. CONCLUSIONS: A total of 14 polymorphic markers suggest a high potential for genetic diversity, gene flow, and mating system analyses in A. colubrina.
Subject(s)
Fabaceae/genetics , Microsatellite Repeats/genetics , Trees/genetics , Tropical Climate , Brazil , Genetic Loci/genetics , Molecular Sequence Data , Polymorphism, Genetic , Species SpecificityABSTRACT
PREMISE OF THE STUDY: This study was designed to develop and characterize a preliminary set of nuclear microsatellite markers for Aspidosperma polyneuron, an endangered tropical tree species. METHODS AND RESULTS: Leaf samples were collected from a group of mature A. polyneuron trees in a large area along the Pardo and Mogi-Guaçu river basins in São Paulo State in the southeastern region of Brazil. Twenty-five simple sequence repeat (SSR) markers were developed and 16 of 30 samples had polymorphic loci. The number of alleles per locus ranged from two to 17 (average = 7.06). A high diversity (H(e) = 0.65) and parentage exclusion probability for the first (0.9995) and second (0.9999) parents were measured. CONCLUSIONS: These 16 polymorphic markers suggest a high potential for genetic diversity, gene flow, and mating system analyses in A. polyneuron.
Subject(s)
Aspidosperma/genetics , DNA, Plant/analysis , Trees/genetics , Aspidosperma/classification , Brazil , Endangered Species , Gene Flow , Genetic Variation , Microsatellite Repeats , Species Specificity , Trees/classificationABSTRACT
Microsatellite loci that were previously developed in the tropical tree Tabebuia aurea were used for the genetic analysis of Tabebuia roseo-alba populations. Nine of 10 simple sequence repeat markers were amplified, and the polymorphism was assessed in 58 individuals sampled from two stands in southeastern Brazil. All loci were polymorphic with Mendelian inheritance. The allele numbers were high, ranging from 5 to 13 in population I and 3 to 7 in population II, with means of 8.9 and 5.5, respectively. We conclude that these markers can be efficiently used for parentage and gene-flow studies.
ABSTRACT
Um total de 119 índios Waiäpi e 348 habitantes de Manaus, Amazonas, foram testados usando nove sistemas genéticos enzimáticos na primeira populaçäo e dois na segunda. Os Waiäpi mostraram algumas diferenças marcantes quanto às médias dos indígenas sul-americanos em ACP*A (1 por cento vs. 14 ñ 9 por cento). GLO1*1 (9 por cento vs. 30 ñ 14 por cento) e ESD*1 e CA2*1 na populaçäo de Manaus mostraram-se dentro do intervalo encontrado previamente na regiäo norte do Brasil (ESD*1: 85 por cento vs. 80-94 por cento; CA2*1: 98 por cento vs. 89-99.8 por cento). As freqüências quase idênticas obtidas para esses dois marcadores nesta populaçäo, quando a amostra foi subdividida de acordo com a cor da pele, sugerem que tais classificaçöes morfológicas têm pouco valor nesta regiäo. Distâncias genéticas entre os Waiäpi e outras tribos, obtidas usando esses e outros marcadores genéticos, mostraram bom paralelismo com as distâncias geográficas que separam esses índios dos outros grupos considerados.
Subject(s)
Humans , Genetic Markers , Genetics, Population , Indians, South American/genetics , Polymorphism, Genetic , Brazil , EnzymesABSTRACT
Estudou-se a distribuiçäo de fenótipos de 11 sistemas genéticos (ACP1, ADA, ESA, PEPA, Hp, Hb, ESD, ABO, Rb, CAII and GLO) em relaçäo à doença de Chagas. Näo foi encontrada associaçäo significante entre esses polimorfismos e a infecçäo por T. cruzi, como também näo se verificou alteraçöes no eletrocardiograma (ECG). Foi detectado um possível efeito biológico do sexo sobre ECG. Por outro lado, um efeito racial na infecçäo, postulado em outro estudo, näo foi confirmado no presente material, indicando ser espuria , essa associaçäo
