ABSTRACT
2 g phenacetin or paracetamol in a single oral dose were administered to five healthy persons under the conditions of antidiuresis and subsequent water diuresis. Excretion of the brush border enzyme GGT, the cytoplasm enzyme LDH, and the lysosomal enzymes, NAG and GAL, was analysed before, during and after ingestion of the analgesics. Increased excretion of LDH and GGT indicated a similar moderate damage of the tubular epithelia after phenacetin and paracetamol. The state of diuresis appeared to have no influence.
Subject(s)
Acetaminophen/pharmacology , Diuresis , Enzymes/urine , Kidney/enzymology , Phenacetin/pharmacology , Acetylglucosaminidase/urine , Humans , Kidney/drug effects , L-Lactate Dehydrogenase/urine , Leucyl Aminopeptidase/urine , Random Allocation , Water/metabolism , beta-Galactosidase/urine , gamma-Glutamyltransferase/urineABSTRACT
A stable liquid control material for urinary enzyme assays was prepared by addition of ethylene glycol (30% v/v) or glycerol (30% v/v) to centrifuged urine, and pH adjustment to 7.0. Tested for 375 days, the material exhibited stable beta-N-acetylglucosaminidase, alanine aminopeptidase, gamma-glutamyltransferase and lactate dehydrogenase activities, at both 4 and -20 degrees C. The material further has been successfully evaluated in an external quality control program.
