Use of the GnRH analogue, deslorelin acetate, in a slow-release implant to accelerate ovulation in oestrous mares.

In two separate controlled clinical trials, the efficacy and safety of 2.2 mg of the GnRH analogue deslorelin, administered subcutaneously as a short-term implant to normally cycling mares in oestrus with a dominant ovarian follicle more than 30 mm in diameter, were evaluated, using a placebo as a negative control. The oestrous cycle of each mare was followed by teasing, palpation per rectum and transrectal ultrasonography. Follicles were monitored every 24 hours by ultrasonography until ovulation occurred. The mares were either mated naturally or inseminated artificially. In trial 1, 174 mares were treated at six locations in Canada, and in trial 2, 98 mares were treated at three locations in the USA. In trial 1, the treatment with deslorelin reduced the mean (sd) time to ovulation from 84.2 (48.4) hours to 50.2 (19.6) hours (P < 0.001) and in trial 2 it reduced it from 88.8 (40.3) hours to 54.1 (26.5) hours (P < 0.001). In trial 1, the percentage of mares ovulating within 48 hours increased from 37.7 per cent in control mares to 86.1 per cent in treated mares (P < 0.001) and in trial 2 the percentage increased from 26.5 to 80.9 per cent (P < 0.001). In trial 2, the duration of oestrus in the deslorelin-treated mares was reduced from 6.1 days to 4.3 days and the number of matings or artificial inseminations was reduced from 2.5 to 1.7 (P < 0.001). In trial 1, days 12 to 20 pregnancy rates for matings at the treatment oestrus were not different for deslorelin-treated (75.6 per cent) and placebo-treated (66.1 per cent) mares. In trial 2, days 12 to 20 pregnancy rates from matings at the treatment oestrus were lower for deslorelin-treated (58.7 per cent) than for placebo-treated (83.3 per cent) mares (P < 0.05), although pregnancy rates were similar for deslorelin-treated (97.1 per cent) and placebo-treated (95.0 per cent) mares after mating at the second oestrus. In both trials, pregnancy losses due to early or late abortions were within the normally expected range and similar for deslorelin-treated (3.6 and 3.7 per cent, respectively) and placebo-treated (13.4 and 7.5 per cent) mares. The treatments did not cause systemic side effects and local reactions at the implantation sites were slight and of short duration.

Contrasting task demands alter the perceived duration of brief time intervals.

Four experiments were performed to assess the effects of task differences on duration judgments. Experiments 1 and 2 used the method of reproduction in prospective, within-subjects designs; their results supported previous research on the effects of task difficulty. Both experiments, using tasks that varied along somewhat different dimensions, found that subjects provided reproduction values that varied inversely with task difficulty. That is, while subjects tended to underreproduce across all tasks, the more difficult the task performed during the target interval, the greater the extent of the underreproduction. Experiments 3 and 4 used a modification of the reproduction method by placing demands upon the subjects during both the target interval and the reproduction phase of each trial; they demonstrated that the greater the degree of contrast between demands made by the task performed during the target interval and those made during reproduction, the less accurate the duration reproduction. The results are discussed in terms of the contextual and resource allocation models of duration estimation.

Quantifying the occurrence of early embryonic mortality on three equine breeding farms.

This prospective field study was designed to describe the incidence of early embryonic mortality (EEM) and factors associated with the cause of EEM on three equine breeding farms in Ontario during the 1989 breeding season. Early embryonic mortality was defined as the loss of a single embryo during the first 40 days of pregnancy (day 0 = day of ovulation or last breeding). Pregnancy diagnoses and subsequent embryonic losses were observed by serial trans-rectal ultrasonography between days 12-20 (PD1) and 21-30 (PD2), and by trans-rectal ultrasonography or palpation per rectum between days 31-40 (PD3). Information on pregnancy status of a mare (or cycle) at 40 days after the last breeding was recorded when available. Nonpregnancy rates were calculated on a per cycle basis, to account for mares with no ultrasonic evidence of an embryo at the initial pregnancy examination. Embryonic mortality rates per cycle were calculated cumulatively (EMR((40))) for the entire 40 day embryonic period and during the specific time periods when a pregnancy diagnosis took place (EMR((PD1)), EMR((PD2)), EMR((PD3))). Embryonic mortality rates were also calculated on a per mare basis for mares experiencing EEM on either their first (EMR((f))) or any (EMR((a))) breeding cycle. Per cycle mare withdrawal rates were calculated cumulatively for the entire 40 day embryonic period (MWR((40))), and at each specific pregnancy diagnosis time period (MWR((PD1)), MWR((PD2)), MWR((PD3))) to account for those breeding cycles in which mares were not able to be observed for the entire forty days of the embryonic period. Records from a total of 699 mares involving 1014 breeding cycles were examined and analyzed. Per cycle risk rates for nonpregnancy (NP) were 36.4%, 45.0%, and 22.1%, for farms 1,2 and 3, respectively. Per cycle EMR((40)) ranged from 8-17%. Per cycle MWR((40)) ranged from 56.5-98.9%, indicative of a high rate of mare withdrawal from the study for the duration of the "embryonic" period. Significant differences (p < 0.05) in EMR((f)) and EMR((a)) per mare existed between two farms, indicative of a farm effect on EMR. Multivariable forward stepwise logistic regression analyses revealed that mares bred on foal heat were 1.9 times more likely than mares not bred on foal-heat to experience EEM (p = 0.008).

Abortion of a mummified fetus associated with short uterine body in a mare.

A mummified fetus was aborted at 284 days of gestation from a Quarter Horse mare with a history of consecutive abortions. The abortion occurred 12 days after administration of supplemental progestogen had been discontinued. Results of a breeding soundness evaluation revealed that this mare had a short uterine body. Other abnormalities were not discovered. This condition may have been responsible for repeated abortions in this mare, and may represent a cause of fetal mummification other than twin pregnancy in mares.

Fertilizing capacity of equine spermatozoa stored for 24 hours at 5 or 20 degrees C.

A breeding trial was conducted to evaluate the effect of in vitro storage time and temperature on fertilizing capacity of equine spermatozoa. Semen obtained from one stallion and diluted with skim milk-glucose extender was used to artificially inseminate 45 estrussynchronized mares. The mares were assigned to one of three treatment groups (15 mares per group): 1) insemination with fresh semen (collected within 0.5 h of use), 2) insemination with semen stored for 24 h at 20 degrees C or 3) insemination with semen stored for 24 h at 5 degrees C. The mares were inseminated daily during estrus, from the detection of a 35-mm follicle until ovulation, with 250 x 10(6) progressively motile spermatozoa (based on initial sperm motility of fresh semen). Semen samples (n = 35) were evaluated prior to insemination for percentages of total sperm motility (TSM), progressive sperm motility (PSM) and sperm velocity (SV). Single-cycle 15-d pregnancy rates. resulting from insemination with fresh semen, from fresh semen stored for 24 h at 20 degrees C or from semen stored for 24 h at 5 degrees C were the same (11 15 ; 73%). Mean diameters (mm) of 15-d embryonic vesicles were not different (P>0.05) among these three treatment groups (21.5 +/- 2.9, 19.6 +/- 2.6 and 20.5 +/- 3.6, respectively). Ten pregnant mares were aborted on Day 15 of gestation for use in another project. The pregnancy status of the 23 remaining pregnant mares was again determined at 35 to 40 d and 55 to 60 d of gestation. No pregnancy losses occurred during this time period. Mean TSM percentages were different (P<0.05) among the three groups: the fresh semen percentage was 89 +/- 2, semen stored for 24 h at 20 degrees C was 57 +/- 11 and semen stored for 24 h at 5 degrees C was 80 +/- 6. Similar differences were found for mean PSM and SV. Semen storage at either 20 or 5 degrees C for 24 h had no apparent effect on the fertilizing capacity of the extended semen samples; however, the reduction in all motility parameters tested was more dramatic in semen stored at 20 degrees C than that stored at 5 degrees C.

Penile paralysis and paraphimosis associated with reserpine administration in a stallion.

Reserpine was administered to an 8-yr-old Thoroughbred stallion at a dosage of 5 mg subcutaneously (s.c.) every 2 wk for a 2-mo period to control unmanageable behavior. Reserpine produced a satisfactory calming effect that lasted for about 2 wk. After the last injection, the stallion developed penile paralysis and was unable to retract his penis, resulting in paraphimosis and attendant penile edema. The prolapsed penis was reduced and kept within the prepuce by placing a purse string suture in the preputial orifice. Phenylbutazone was given orally (1 gm) and the stallion was exercised for 20 to 30 min twice daily. This treatment was continued for 20 d with little improvement. The purse string retention suture cut through the skin 5 d after the stallion was discharged from the clinic. The penis was then supported against the abdominal wall and the stallion was exercised by hand for 30 min each day. The stallion was not used for breeding within 34 mo after the last injection of reserpine. A breeding soundness examination was performed approximately 3 yr after the initial injury. At this time the stallion's penis was noted to extend 5 to 8 cm from the prepuce when in a detumescent state. Although the stallion protruded his penis when exposed to a mare in estrus, a full rigid erection was never attained. Examination of the penis revealed partial engorgement of the corpus cavernosum penis and a 2.5-cm-wide dorsal semi-circumferential depression of the penile shaft approximately 10 to 12 cm proximal to the glans penis. The penile shaft and glans penis distal to this depression were cooler than the proximal portion of the penis. Semen collection was attempted, aided by manual insertion of the penis into the artificial vagina. When serving the artificial vagina, no "belling" of the glans penis was observed, although ejaculation occurred. Semen evaluation indicated normal spermatozoal motility and morphology parameters. The stallion was able to breed several mares with manual assistance to guide the penis into the vagina and one mare was diagnosed pregnant.

Hormonal changes in sows after induced porcine parvovirus infection in early pregnancy.

Hormonal changes, lesions, and virus isolation studies were determined in sows after uterine artery inoculation with porcine parvovirus [( PPV], strain NADL-8) in early pregnancy. Two sows were given PPV on days 14 or 16 and were euthanatized and necropsied on day 35 after twice daily plasma collection for hormone measurement. Parvovirus was given to 4 sows on day 14 and to 4 sows on day 21 with 5 times daily plasma samples collected for 1 week. Sows were examined on days 21 and 28, respectively. Four control sows in each group on days 14 and 21 were given a placebo injection and were similarly studied. All embryos in all but 1 sow given PPV were in various stages of resorption at necropsy. Normal embryos were present in all control sows. Estrone sulfate values increased logarithmically, progesterone values remained stable, and concentrations of 13, 14-dihydro-15-keto-prostaglandin (PG) F2 alpha (PGFM), a PGF2 alpha metabolite, were less than 200 ng/ml for sows given a placebo. In contrast, sows with resorbing embryos did not have an increase in estrone sulfate values. A decrease in plasma progesterone values occurred in 9 of 10 sows inoculated with PPV; this decrease was accompanied by greater than or equal to 1 marked increase in PGFM concentrations. Quantitative assessment of the uterus revealed significantly greater cytoplasmic density in endometrial and glandular cell (P less than 0.01), a greater glandular epithelium height (P less than 0.05), and twice the number of glands (P less than 0.05) in control sows, compared with values in sows inoculated with PPV.(ABSTRACT TRUNCATED AT 250 WORDS)