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1.
Cell Rep ; 32(3): 107907, 2020 07 21.
Article in English | MEDLINE | ID: mdl-32698008

ABSTRACT

During development of the central nervous system (CNS), neurons polarize and rapidly extend their axons to assemble neuronal circuits. The growth cone leads the axon to its target and drives axon growth. Here, we explored the mechanisms underlying axon growth in three dimensions. Live in situ imaging and super-resolution microscopy combined with pharmacological and molecular manipulations as well as biophysical force measurements revealed that growth cones extend CNS axons independent of pulling forces on their substrates and without the need for adhesions in three-dimensional (3D) environments. In 3D, microtubules grow unrestrained from the actomyosin cytoskeleton into the growth cone leading edge to enable rapid axon extension. Axons extend and polarize even in adhesion-inert matrices. Thus, CNS neurons use amoeboid mechanisms to drive axon growth. Together with our understanding that adult CNS axons regenerate by reactivating developmental processes, our findings illuminate how cytoskeletal manipulations enable axon regeneration in the adult CNS.


Subject(s)
Axons/metabolism , Central Nervous System/metabolism , Actins/metabolism , Actomyosin/metabolism , Animals , Cell Adhesion , Cell Polarity , Collagen/metabolism , Fibroblasts/metabolism , Growth Cones/metabolism , Hippocampus/embryology , Mice, Inbred C57BL , Microtubules/metabolism , Neuronal Outgrowth , Polymerization
2.
Nat Protoc ; 9(5): 1028-37, 2014 May.
Article in English | MEDLINE | ID: mdl-24705599

ABSTRACT

An understanding of the molecular mechanisms of axon regeneration after injury is key for the development of potential therapies. Single-cell axotomy of dissociated neurons enables the study of the intrinsic regenerative capacities of injured axons. This protocol describes how to perform single-cell axotomy on dissociated hippocampal neurons containing synapses. Furthermore, to axotomize hippocampal neurons integrated in neuronal circuits, we describe how to set up coculture with a few fluorescently labeled neurons. This approach allows axotomy of single cells in a complex neuronal network and the observation of morphological and molecular changes during axon regeneration. Thus, single-cell axotomy of mature neurons is a valuable tool for gaining insights into cell intrinsic axon regeneration and the plasticity of neuronal polarity of mature neurons. Dissociation of the hippocampus and plating of hippocampal neurons takes ∼2 h. Neurons are then left to grow for 2 weeks, during which time they integrate into neuronal circuits. Subsequent axotomy takes 10 min per neuron and further imaging takes 10 min per neuron.


Subject(s)
Axotomy/methods , Hippocampus/cytology , Nerve Regeneration/physiology , Neural Pathways/cytology , Single-Cell Analysis/methods , Animals , Fluorescence , Mice
3.
Neuron ; 76(6): 1091-107, 2012 Dec 20.
Article in English | MEDLINE | ID: mdl-23259946

ABSTRACT

Neurites are the characteristic structural element of neurons that will initiate brain connectivity and elaborate information. Early in development, neurons are spherical cells but this symmetry is broken through the initial formation of neurites. This fundamental step is thought to rely on actin and microtubule dynamics. However, it is unclear which aspects of the complex actin behavior control neuritogenesis and which molecular mechanisms are involved. Here, we demonstrate that augmented actin retrograde flow and protrusion dynamics facilitate neurite formation. Our data indicate that a single family of actin regulatory proteins, ADF/Cofilin, provides the required control of actin retrograde flow and dynamics to form neurites. In particular, the F-actin severing activity of ADF/Cofilin organizes space for the protrusion and bundling of microtubules, the backbone of neurites. Our data reveal how ADF/Cofilin organizes the cytoskeleton to drive actin retrograde flow and thus break the spherical shape of neurons.


Subject(s)
Actin Depolymerizing Factors/physiology , Actins/metabolism , Cell Shape/physiology , Cerebral Cortex/embryology , Destrin/physiology , Growth Cones/metabolism , Neurites/metabolism , Animals , Biological Transport , Cell Growth Processes/physiology , Cells, Cultured , Cerebral Cortex/cytology , Hippocampus/cytology , Hippocampus/embryology , In Vitro Techniques , Mice , Mice, Knockout , Microtubules/physiology , Neurogenesis/physiology
4.
J Neurosci ; 27(48): 13117-29, 2007 Nov 28.
Article in English | MEDLINE | ID: mdl-18045906

ABSTRACT

The establishment of polarity is an essential process in early neuronal development. Although a number of molecules controlling neuronal polarity have been identified, genetic evidence about their physiological roles in this process is mostly lacking. We analyzed the consequences of loss of Cdc42, a central regulator of polarity in multiple systems, on the polarization of mammalian neurons. Genetic ablation of Cdc42 in the brain led to multiple abnormalities, including striking defects in the formation of axonal tracts. Neurons from the Cdc42 null animals sprouted neurites but had a strongly suppressed ability to form axons both in vivo and in culture. This was accompanied by disrupted cytoskeletal organization, enlargement of the growth cones, and inhibition of filopodial dynamics. Axon formation in the knock-out neurons was rescued by manipulation of the actin cytoskeleton, indicating that the effects of Cdc42 ablation are exerted through modulation of actin dynamics. In addition, the knock-outs showed a specific increase in the phosphorylation (inactivation) of the Cdc42 effector cofilin. Furthermore, the active, nonphosphorylated form of cofilin was enriched in the axonal growth cones of wild-type, but not of mutant, neurons. Importantly, cofilin knockdown resulted in polarity defects quantitatively analogous to the ones seen after Cdc42 ablation. We conclude that Cdc42 is a key regulator of axon specification, and that cofilin is a physiological downstream effector of Cdc42 in this process.


Subject(s)
Actin Depolymerizing Factors/metabolism , Neurons/physiology , cdc42 GTP-Binding Protein/physiology , Actins/metabolism , Amino Acid Chloromethyl Ketones/pharmacology , Animals , Apoptosis/physiology , Axons/physiology , Cell Polarity/drug effects , Cell Polarity/physiology , Cells, Cultured , Cysteine Proteinase Inhibitors/pharmacology , Embryo, Mammalian , Gene Expression Regulation/physiology , Green Fluorescent Proteins/metabolism , Hippocampus/cytology , Mice , Mice, Knockout , Microtubule-Associated Proteins , Phosphorylation , RNA Interference/physiology , cdc42 GTP-Binding Protein/deficiency
5.
J Biol Chem ; 277(7): 5637-43, 2002 Feb 15.
Article in English | MEDLINE | ID: mdl-11741885

ABSTRACT

Polarized cells such as neurons and endothelial cells appear to be involved in two invariant pathological features of Alzheimer's disease pathology, namely the formation of senile plaques and cerebral amyloid angiopathy. This implicates polarized sorting mechanisms in the production and accumulation of amyloid beta-peptide (Abeta). We have now studied polarized sorting of beta-secretase (BACE) in Madin-Darby canine kidney (MDCK) cells. The majority of BACE is sorted to the apical surface of MDCK cells where very little beta-amyloid precursor protein (betaAPP) is observed, because betaAPP undergoes basolateral sorting. Consistent with the usage of similar mechanisms for polarized sorting, BACE was also found to be targeted to axons of hippocampal neurons. The remaining basolaterally sorted BACE competes with the highly polarized basolateral alpha-secretase activity. Therefore, substantial amounts of BACE are targeted away from betaAPP to a non-amyloidogenic compartment, a cellular mechanism that limits Abeta generation. In addition, no alpha-secretase activity was observed on the apical side whereas gamma-secretase activity is observed on the basolateral and the apical side. Consistent with this finding, substantial amounts of Abeta can be produced apically upon missorting of betaAPP to the apical surface. These data demonstrate that Abeta production is limited in polarized cells by differential targeting of BACE and its substrate betaAPP. Moreover, our findings suggest that betaAPP may not be a major physiological substrate of BACE.


Subject(s)
Amyloid beta-Peptides/chemistry , Aspartic Acid Endopeptidases/chemistry , Aspartic Acid Endopeptidases/metabolism , Peptides/chemistry , Amino Acid Sequence , Amyloid Precursor Protein Secretases , Animals , Cell Line , Cell Membrane/enzymology , Cells, Cultured , DNA, Complementary/metabolism , Dogs , Endopeptidases/metabolism , Hippocampus/metabolism , Immunohistochemistry , Microscopy, Confocal , Models, Biological , Molecular Sequence Data , Neurons/metabolism , Precipitin Tests , Protein Binding , Rats , Time Factors
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