Subject(s)
Burns/complications , Heparin/administration & dosage , Pancreatitis/complications , Plasma Substitutes/administration & dosage , Procaine/administration & dosage , Toxemia/drug therapy , Acute Disease , Drug Therapy, Combination , Humans , Infusions, Parenteral , Lymphatic System , Toxemia/etiologyABSTRACT
The article describes the morphological basis of the positive effect of the treatment of experimental pancreonecrosis, reproduced on dogs, by single endolymphatic injection of 5- fluorouracil. The index of the enzymatic activity in the pancreas correlated with the morphological data.
Subject(s)
Fluorouracil/administration & dosage , Pancreas/pathology , Animals , Biopsy , Dogs , Drug Evaluation, Preclinical , Injections, Intralymphatic , Microscopy, Electron , Necrosis , Pancreas/drug effects , Time FactorsABSTRACT
Histological and electron microscopic studies of canine pancrease were conducted with biochemical control of pancreatic enzyme activity before and after treatment of experimental hemorrhagic pancreatonecrosis. 30-60 minutes after the disease induction microfocal pancreatonecrosis was revealed, while 6-8 hours later subtotal hemorrhagic pancreatonecrosis with changes in microcirculatory vessels of the pancreas manifested in erythrostasis, thrombosis and fibrinoid wall necrosis accompanied by the increase of pancreatic enzyme activity and decrease in blood, lymph and serum antienzyme activity developed. The treatment of experimental pancreatonecrosis with endolymphatic injection of contrical resulted in the morphologically confirmed attenuation of the necrosis intensity, limitation of necrotic zone size, blood circulation recovery in microcirculatory system, marked decrease of proteolytic enzyme activity and increase of blood serum and central lymph antienzyme activity.
Subject(s)
Aprotinin/therapeutic use , Pancreas/pathology , Protease Inhibitors/therapeutic use , Animals , Aprotinin/administration & dosage , Dogs , Injections, Intralymphatic , Necrosis , Pancreas/drug effects , Protease Inhibitors/administration & dosageABSTRACT
Experiments on 12 dogs with experimental acute hemorrhagic pancreatitis were made to assess the effect of endolymphatic therapy with 5-fluorouracil (5-FU) in a dose of 5 mg/kg. The changes in the activity of pancreatic enzymes and kinins in the blood, lymph and urine were studied before and after endolymphatic administration of 5-FU. After the dogs were sacrificed the pieces of endocrine tissue from different parts of the pancreas were exposed to morphological study. Endolymphatic administration of 5-FU for experimental acute pancreatitis reduced the activity of proteo- and lipolytic enzymes. The structural manifestation of the enzymological changes included the delimitation of the areas of necrosis in acinar tissue of the pancreas, improvement of intraorgan blood and lymph circulation.
