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1.
J Hosp Infect ; 73(2): 121-8, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19716628

ABSTRACT

Listeria monocytogenes is a foodborne bacterial pathogen. Immunocompromised patients are at higher risk of developing invasive listeriosis with high fatality rates. After notification of two patients with Listeria that had stayed in the same hospital (hospital A) before the onset of infection, we began an investigation to ascertain the extent of the outbreak, identify its source and prevent further infections. We conducted active case finding by contacting hospital A, reviewing medical records and retrospectively investigating listeriosis notifications from the German surveillance system (SurvNet). The kitchen (hospital A) and its meat supplier (company X) were inspected and environmental and food samples were taken for microbiological testing. All isolates of L. monocytogenes, together with patient and food-related isolates from Baden-Württemberg 2006 to 2008, were characterised by pulsed-field gel electrophoresis (PFGE). Altogether, 16 cases of listeriosis were identified. Serotype 4b with the indistinguishable PFGE patterns (AscI 17a/ApaI 10) was detected from nine patients, five environmental and three ready-to-eat scalded sausage samples from company X, and two food samples from hospital A. All 11 patient cases linked to hospital A were immunosuppressed and were regularly served food during their hospital stay. Ten of these patients received corticosteroids and proton pump inhibitors (PPIs). Five cases were fatal. Our investigations indicate that ready-to-eat scalded sausages from company X caused this outbreak of listeriosis. Hospital food suppliers should guarantee the absence of L. monocytogenes in ready-to-eat meat products, controlled through optimised quality assurance.


Subject(s)
Community-Acquired Infections/epidemiology , Cross Infection/epidemiology , Disease Outbreaks , Listeria monocytogenes , Listeriosis/epidemiology , Meat Products/microbiology , Aged , Aged, 80 and over , Animals , Community-Acquired Infections/microbiology , Cross Infection/microbiology , Electrophoresis, Gel, Pulsed-Field , Female , Food Contamination/analysis , Food Industry , Food Microbiology , Food-Processing Industry , Foodborne Diseases/epidemiology , Foodborne Diseases/microbiology , Humans , Immunocompromised Host , Listeria monocytogenes/classification , Listeria monocytogenes/genetics , Listeria monocytogenes/isolation & purification , Listeriosis/microbiology , Male , Medical Records , Middle Aged , Serotyping
3.
Ophthalmic Res ; 28(1): 50-6, 1996.
Article in English | MEDLINE | ID: mdl-8726677

ABSTRACT

We developed a method to culture mouse corneal epithelium. Cultured cells tested by 1-D-SDS-PAGE exhibited protein mobility patterns similar to freshly isolated epithelia. Western blots with antibodies broadly recognizing cytokeratins showed a similar pattern for both fresh and cultured cells, but only the fresh sample stained with J7, specific for a 55-kD 'corneal' cytokeratin. Cultured cells examined at confluency by transmission electron microscopy exhibited desmosomal contacts typical of epithelia. The ability to culture mouse corneal epithelial tissue will be useful for studies requiring large amounts of material by reducing animal numbers.


Subject(s)
Cornea/cytology , Cornea/metabolism , Animals , Cells, Cultured , Cornea/ultrastructure , Electrophoresis, Polyacrylamide Gel , Epithelial Cells , Epithelium/metabolism , Epithelium/ultrastructure , Female , Fluorescent Antibody Technique , Immunohistochemistry , Mice , Mice, Inbred Strains , Microscopy, Electron
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