ABSTRACT
A method for the isolation of six isoflavones (genistein, genistin, daidzein, daidzin, glycitein and glycitin) with high purity from Semen sojae praeparatum, a famous traditional Chinese medicine, by preparative HPLC is described.
Subject(s)
Drugs, Chinese Herbal/chemistry , Phytotherapy , Plants, Medicinal , Chromatography, High Pressure Liquid , Fermentation , Humans , Isoflavones/chemistry , Structure-Activity RelationshipABSTRACT
AIM: To study the chemical constituents of Marsdenia tenacissima (Roxb.) Wight et Arn. METHODS: To separate compounds with various chromatography technology and to elucidate their structures by chemical and spectral analysis. RESULTS: Two compounds were isolated from Marsdenia tenacissima and their stuctures were determined as tenacissosides J (I) and tenacissosides K (II). CONCLUSION: Compounds I and II are new C21 steroidal glycosides.
Subject(s)
Glycosides/isolation & purification , Marsdenia/chemistry , Plants, Medicinal/chemistry , Steroids/isolation & purification , Glycosides/chemistry , Molecular Conformation , Molecular Structure , Plant Stems/chemistry , Steroids/chemistryABSTRACT
OBJECTIVE: AIM To study the chemical constituents of the fruit of Eucalyptus globulus. METHOD: Various column chromatographies with silica gel were employed for the isolation and purification. The structures of the compounds were elucidated with of spectral analyses and chemical methods. RESULT: Five compounds were isolated and elucidated as follows: betulonic acid (I), betulinic acid (II), ursolic acid (III), corosolic acid (IV), daucosterol (V). CONCLUSION: Compound I, II, III and IV were isolated from this plant for the first time.
