ABSTRACT
Aim of this study was to analyze the state of coronary arteries in patients with acute coronary syndrome according to polymorphism of integrin ß-3 (ITGB3) gene. All patients were divided into 2 groups: carries and non-carries of PLA2 allele. Carriers of PLA2 allele compared with noncarriers had lesser grades of coronary artery stenoses but greater number of involved arteries. Carriers had more repetitive acute coronary events.
Subject(s)
Acute Coronary Syndrome , Coronary Vessels/pathology , Integrin beta3/genetics , Acute Coronary Syndrome/diagnosis , Acute Coronary Syndrome/genetics , Acute Coronary Syndrome/physiopathology , Aged , Coronary Angiography , Female , Genetic Carrier Screening , Heterozygote , Humans , Male , Middle Aged , Polymorphism, Genetic , Recurrence , Severity of Illness IndexABSTRACT
PLA polymorphism of platelet integrin receptor, GpIIIa glycoprotein, (PLA polymorphism of the ITGB3 gene) is associated with the risk of myocardial infarction and CHD especially in young subjects. We analyzed ITGB3 gene polymorphism in patients with acute coronary insufficiency. It was shown that increased AP and altered blood lipid spectrum in the acute period of disease in carriers of the PLA allele (PLA1/PLA2 and PLA2/PLA2 genotypes) can be regarded as manifestations of stress reaction. The data obtained indicate that the PLA2 allele is a predictor of complications of acute coronary insufficiency. This observation is of importance for the choice of adequate therapy for the patients with this disorder.
Subject(s)
Acute Coronary Syndrome/genetics , DNA/genetics , Hypertension/epidemiology , Integrin beta3/genetics , Polymorphism, Genetic , Acute Coronary Syndrome/complications , Aged , Aged, 80 and over , Alleles , Female , Genotype , Humans , Hypertension/complications , Hypertension/genetics , Incidence , Integrin beta3/metabolism , Male , Middle Aged , Risk Factors , Russia/epidemiologyABSTRACT
The authors analyse effect of GPIIIA gene (PI a allele) polymorphism on the frequency of complicated coronary heart disease in patients with dyslipidemia and hypertensive disease. Specific features of ventricular repolarization (T-wave variability) in patients with acute coronary syndrome are described.
Subject(s)
Acute Coronary Syndrome/genetics , DNA/genetics , Electrocardiography , Integrin beta3/genetics , Polymorphism, Genetic , Acute Coronary Syndrome/complications , Acute Coronary Syndrome/physiopathology , Adult , Aged , Alleles , Dyslipidemias/blood , Dyslipidemias/complications , Dyslipidemias/genetics , Gene Frequency , Humans , Hypertension/blood , Hypertension/complications , Hypertension/genetics , Integrin beta3/blood , Middle Aged , Polymerase Chain Reaction , PrognosisABSTRACT
We studied the role of integrins, primarily, the role of allele distribution of GP3a gene in development of prostatic cancer (PC) and assessment of its prognostic significance. From November 2003 to May 2004 we examined 32 patients with PC: 11 patients with local PC T1-2N0M0; 14 patients with locally advanced cancer T3N0M0 and 7 patients with invasive and/or metastatic cancer T3-4N10-1 or T3-4N0-1M1. The blood from all the patients we studied with PCR for alleles of GP3a gene, PSA. Seventeen patients were found to have alleles PLA1A1, 14(44%)--alleles PLA1A2, 1(3%)--alleles PLA2A2. Alleles PLA1A2 occurred significantly more often than in the population (p < 0.005). The group analysis has found that 8 patients with local PC had alleles PLA1A1, 3 patients--alleles PLA1A2 (27%). We discovered alleles PLA2A2, PLA1A1 and PLA1A2 in 1(7%), 5(36%) and 8(57%) patients with locally advanced PC, respectively. Among patients with metastatic and/or invasive prostatic cancer, there were 4 (57%) and 3 (43%) cases of alleles PLA1A1 and PLA1A2, respectively. Our study demonstrated influence of carriage of PLA2 allele on occurrence of PC risk (5-fold higher) and its invasive forms (10-fold higher and more). Probability to develop local invasion among patients with prostatic cancer--carriers allele PLA1A2 is 6 times higher than among carriers of alleles PLA1A1. A PC course in carriers of alleles PLA1A2 may be characterized by faster development of local invasion and metastasizing vs carriers of alleles PLA1A1. These findings can be used in design of nomograms for prognostication of invasion of clinically small tumors in verification of significance on greater number of the patients.
Subject(s)
Antigens, Human Platelet/genetics , Integrin beta3/genetics , Prostatic Neoplasms/diagnosis , Aged , Aged, 80 and over , Alleles , Genetic Predisposition to Disease , Humans , Male , Middle Aged , Neoplasm Invasiveness/diagnosis , Neoplasm Staging , Pilot Projects , Prognosis , Prostatic Neoplasms/genetics , Risk FactorsABSTRACT
On the basis of transfer factor pAP42 and nonconjugative plasmids pRSF2124 and pUB781, the cointegrative plasmids pAP42/pRSF2124 and pAP42/pUB781 were constructed. Complex systems of plasmid transfer inhibition (funU and finV) were detected in the structure of cointegrative plasmids.
Subject(s)
DNA Transposable Elements/genetics , Escherichia coli/genetics , Gene Expression Regulation, Bacterial/genetics , Plasmids/genetics , Replicon/genetics , Conjugation, Genetic/genetics , F Factor/geneticsABSTRACT
A study was made of the ability of reference plasmids of the 6 known Fin-groups to inhibit the functions of transfer genes (tra-genes) of the 4 derepressed F-like plasmids (pAP22-2, pAP38, pAP43, pAP53). It was shown that unlike the derepressed Flac plasmid, the conjugation transfer of pAP38 and pAP53 plasmids was inhibited only by, the FinV plasmid, whereas pAP22-2 plasmids by Fin V and Fin V plasmids. The formation of donor-specific pili in case of pAP38 plasmid was inhibited by Fin Q, Fin U and Fin V plasmids, in case of pAP43 plasmid by Fin U Fin V and Fin W plasmids.
