ABSTRACT
Paired wavelength relevance as spectrophotometric strategy was carried for simultaneous analysis of unresolved bands of doxycycline hydrochloride (DOX) and tylosin tartrate (TYT) as an example of veterinary binary mixture. These methods based on the relation between two points on the scanned or manipulated spectra (derivative, ratio). The methods using the absorbance difference between two wavelengths namely, dual wavelength (DW), induced dual wavelength (IDW), absorption correction (AC) and advanced absorbance subtraction (AAS) or amplitude difference namely, ratio difference (RD), amplitude subtraction namely, amplitude correction (PC) and advanced amplitude modulation (AAM) as well as amplitude addition namely, derivative ratio (DD1). Calibration graphs are direct relation in the range of (5-40) µg/mL for doxycycline hydrochloride and (5-45) µg/mL for tylosin tartrate. The suggested methods was successfully applied for evaluate the potency of their veterinary medicine that has direct affecting human health. The method has been validated according to the guideline of international conference on Harmonization and parameters like linearity, range, accuracy and precision have been studied. The outcomes were matched statistically with those of the authorized systems; Student's t-test, F-test, and One-way ANOVA, presenting acceptable values and no momentous change with accuracy. The procedures can be considered useful for the quantitative analysis.
