Extracellular Distribution of Collagen II and Perifibrillar Adapter Proteins in Healthy and Osteoarthritic Human Knee Joint Cartilage.

Perifibrillar adapter proteins, interconnecting collagen fibrils, and linking the collagen network with the aggrecan matrix seem to play a crucial role in the pathogenesis of osteoarthritis (OA). Therefore, we examined immunohistochemically the extracellular distribution of collagen II and the main perifibrillar adapter proteins-collagen IX, decorin, cartilage oligomeric matrix protein (COMP), and matrilin-3-in human samples of healthy (n=4) and OA (n=42) knee joint cartilage. Histopathology assessment was performed using an OA score. Staining patterns were evaluated in relation to the disease stage. The perifibrillar adapter proteins were uniformly distributed in the upper zones of healthy cartilage. In moderate OA (n=8; score 14.3 ± 4.7), all proteins analyzed were locally absent in the fibrillated area or the superficial and upper mid zone. In advanced OA (n=20; score 18.9 ± 5.3), they were uniformly distributed in these zones and accumulated pericellularly. Perifibrillar adapter proteins are important for the stabilization of the collagen network in the upper zones of healthy cartilage. Their degradation might be a critical event in early OA. In advanced OA, there are indications for an increased synthesis in an attempt to regenerate the lost tissue and to protect the remaining cartilage from further destruction.

Experimental studies on the tensile properties of human umbilical cords.

When tried in court, mothers accused of neonaticide may claim that the umbilical cord just broke during birth and the newborn child bled to death accordingly. To evaluate the possibility of a breakage of the umbilical cord is the goal of this work. Therefore 25 umbilical cords from neonates of both sexes born at term were stretched using an electrically operated material testing machine and the energy necessary to break them was measured. This experimental set-up equals a static strain, not a dynamic one. The maximum force endured (F max) ranged from 37.24 N to 150.04 N. The average force endured was 79.87 N with a standard deviation of 27.39. The elongation at break varied from 13.24% to a maximum of 119.93%. We found no relationship between the force endured and any of the following parameters: birth weight, pH of the venous umbilical blood, diameter of cord, free length under testing, duration of pregnancy or the mother's age. We performed a literature research and tried to define the circumstances in which a break is more likely to occur, these being malformations, entanglement or disease, e.g. inflammation.

Morphology of the first metatarsal head and hallux rigidus: a cadaveric study.

PURPOSE: Treatment options for hallux rigidus include several conservative and surgical measures. The aetiology leading to the disease has not sufficiently been examined so far. MATERIALS AND METHODS: We analysed the anatomical configuration of the first metatarsal head of 120 metatarsal bones of different collectives aiming to find a possible correlation between the geometry of the first metatarsophalangeal joint and manifestation of hallux rigidus. Wet human cadaveric specimens and macerated dry specimens served as material. The relevant parameters used for analysis were an axis running through the metatarsal head, the anatomical longitudinal axis, and the radius of curvature of the first metatarsal bone. RESULTS: A significant difference was found in the radius of curvature of osteoarthritic and healthy subjects. Using the binary logistic regression, we were able to predict the probability of an occurrence of hallux rigidus in dependence of the radius of curvature. Furthermore, we were enabled to calculate a correct prediction for the appearance of osteoarthritis in 85 % of the healthy subjects, and 73 % of the osteoarthritic subjects. CONCLUSIONS: A consolidated view of the factors indicates that persons with a high risk for the appearance of hallux rigidus should be identified by measuring the radius of curvature in conventional radiographs and preventive measures to postpone the occurrence of clinically relevant hallux rigidus considered.

Femoral and obturator nerves palsy caused by pelvic cement extrusion after hip arthroplasty.

Cement extrusion into the pelvis with subsequent palsy of the obturator and femoral nerves is a rare entity after hip replacement surgery. Cemented fixation of the acetabular cup has been considered as a safe and reliable standard procedure with very good long term results. We present a case of fifty year old female patient after hip arthroplasty procedure which suffered an obturator and femoral nerve palsy caused by extrusion of bone cement into the pelvis. Postoperative X-rays and CT-scan of the pelvis demonstrated a huge mass consisted of bone cement in close proximity of femoral and obturator nerves. The surgery charts reported shallow and weak bony substance in postero-superior aspect of the acetabulum. This weak bony acetabular substance may have caused extrusion of bone cement during press-fitting of the polyethylene cup into the acetabulum, and the following damage of the both nerves produced by polymerization of bone cement. The bone cement fragment has been surgically removed 3 weeks after arthroplasty. The female patient underwent intensive postoperative physical therapy and electro stimulation which resulted in full recovery of the patient to daily routine and almost normal electromyography results.

Relationship between human femorotibial joint configuration and the morphometry of the anterior cruciate ligament.

INTRODUCTION: Individual variations in the anatomy of the knee joint have been suggested to affect the ability to functionally compensate for ACL insufficiency or to put an individual at an increased risk of ACL injury. These variations include the posterior tibial slope, the concavity of the medial tibial plateau, the convexity of the lateral tibial plateau, and the configuration of the femoral condyles. METHOD: This anatomical study investigates if there is a correlation between the individual surface geometry of the femorotibial joint and the morphometry of the ACL. These data were assumed to provide evidence whether or not the functional stability of an ACL-insufficient knee may be derived from its radiographic surface geometry. Standardised measurement techniques were used to analyse the surface geometry of 68 human cadaver knees. Data were correlated with the cross-sectional area, the area of insertion and position of the footprint of the ACL and its functional bundles. RESULTS: Analysis revealed that there was a significant, but weak correlation between the femoral and tibial area of ACL insertion and the depth of the medial and lateral femoral condyle. No correlation was found between the surface geometry of the femorotibial joint and the cross-sectional area of the ACL. The results of this anatomical study suggest that the relationship between the joint surfaces and the morphometry of the ACL primarily is a function of size of the knee joint. CONCLUSIONS: Based on our results, there is no evidence that the stability of the knee can be derived from its radiographic surface geometry.

Morphometric and biomechanical comparison of tendons used for interposition arthroplasty in carpometacarpal arthritis of the thumb.

PURPOSE: To identify the wrist tendon most effectively maintaining the trapezial space in interpositional arthroplasty in osteoarthritis of the carpometacarpal joint of the thumb. METHODS: The morphometrics of the os trapezium and the tendons of the flexor carpi radialis, extensor carpi radialis longus, and abductor pollicis longus were determined. The stiffness and compressive loading characteristics of the rolled-up tendons were compared to the os trapezium. RESULTS: No significant morphometric differences between the three tendons were found. The mass and volume of the trapezium was significantly larger when compared to the tendon balls. No significant differences in the compressive loading resistance were found between the tendons, but the mean stiffness was 85% lower when compared to the os trapezium. CONCLUSIONS: Neither tendon material approached the volume nor the stiffness provided by the os trapezium. Any tendon is considered to insufficiently maintain the trapezial space following trapeziectomy.

In vitro comparison of the pullout strength of 3 anterior double-screw fixation techniques with different screw angulations.

OBJECT: The pullout resistance of double-screw fixation systems in anterior spine surgery has been shown to be dependent on screw length as well as on screw angulation. The objective of the study was to evaluate the pullout strength of anterior double-screw systems with different angulations. METHODS: The authors conducted a comparative pullout test of converging, parallel, and diverging angulations of double-screw systems in human cadavers. Twenty-four human vertebral bodies from T-11 to L-1 were harvested from 8 donors, dissected from surrounding tissue, and matched to 3 different fixation groups. Three systems were tested: VentroFix, with near parallel screw direction; the Hopf Anterior Fixation System (HAFS), with converging screw angulation; and the ART anterior system, with diverging screw angulation. RESULTS: The mean (± SD) pullout strength of the VentroFix system was 699 ± 214 N, whereas the HAFS resisted to 591 ± 372 N. The ART anterior system with diverging screws demonstrated a pullout resistance of 810 ± 273 N. There was no significant difference amongst the pullout forces of the 3 groups (p > 0.05). In the HAFS and the ART anterior group, a weak correlation of pullout strength and bone mineral density measured by quantitative CT was found (r = 0.59 and r = 0.62, respectively), whereas the pullout force of the VentroFix system was not correlated with bone mineral density (r = 0.33). CONCLUSIONS: The in vitro pullout resistance of anterior double-screw systems does not appear to depend on screw angulation.

Human knee joint anatomy revisited: morphometry in the light of sex-specific total knee arthroplasty.

This study investigates differences in the anatomy of male and female knee joints to contribute to the current debate on sex-specific total knee implants. Morphometric data were obtained from 60 human cadaver knees, and sex differences were calculated. All data were corrected for height, and male and female specimens presenting with an identical length of the femur were analyzed as matched pairs. Male linear knee joint dimensions were significantly larger when compared with females. When corrected for differences in height, medial-lateral dimensions of male knees were significantly larger than female; however, matched paired analysis did not prove these differences to be consistent. Although implant design should focus interindividual variations in knee joint anatomy, our data do not support the concept of a female-specific implant design.

[Imageless computer navigation of hip resurfacing arthroplasty]. / Bildfreie Computernavigation von Hüftoberflächenersatzprothesen.

OBJECTIVE: Precise implantation of hip resurfacing arthroplasty by imageless computer navigation. Hence a malalignment of the femoral component, leading to early loss of the implant, can safely be avoided. INDICATIONS: Coxarthrosis in patients with normal bone mineral density; only minor deformity of the femoral head that enables milling around the femoral neck without notching. CONTRAINDICATIONS: Osteoporosis; large necrosis of the femoral head; metal allergy; small acetabular seat and corresponding wide femoral neck, leading to needless acetabular bone loss; pregnancy, lactation. SURGICAL TECHNIQUE: Hip joint exposure by a standard surgical approach, bicortical placement of a Schanz screw for the navigation array in the lesser trochanter. Referencing of the epicondyles, the four planes around the femoral neck and head by use of the navigation pointer. Planning of the desired implant position on the touchscreen of the navigation device; a guide wire is inserted into the femoral head and neck using the navigated drill guide; navigated depth drilling is performed. The femoral head is milled using the standard instruments. The acetabular bone stock is prepared with the conventional instrumentation; high-viscosity cement is finger-packed on the reamed head and the femoral component is inserted. Hammer blows should be avoided to prevent microfractures. Verification of the implant position by the navigation device; displacement of the Schanz screw; joint reposition and closure of the wound. POSTOPERATIVE MANAGEMENT: Standard postoperative management after hip arthroplasty. RESULTS: The comparison of 40 navigated and 32 conventionally implanted ASR prostheses resulted in a significant reduction of outliers by use of computer navigation (navigated procedures: one outlier, conventional procedure: nine outliers; p<0.001). Accuracy of the navigation device was tested by analysis of planned and verified implant position: CCD angle accuracy was 1 degrees , antetorsion accuracy was 1 degrees , and offset accuracy was 1.5 mm. An ongoing computed tomography-based anatomic study proved a varus-valgus accuracy of the navigation device of 1 degrees .

The epidemiology, etiology, diagnosis, and treatment of osteoarthritis of the knee.

BACKGROUND: Osteoarthritis is the most common joint disease of adults worldwide. Its incidence rises with age. Both intrinsic and extrinsic risk factors promote its development. In men aged 60 to 64, the right knee is more commonly affected; in women, the right and left knees are affected with nearly equal frequency. METHODS: The PubMed, Medline, Embase and Cochrane Library databases were selectively searched for current studies (up to September 2009; case reports excluded) on the epidemiology, etiology, diagnosis, staging, and treatment of osteoarthritis of the knee. The search terms were "gonarthrosis," "prevention," "conservative treatment," "joint preservation," "physical activity," "arthroscopy," "osteotomy," "braces," "orthoses," and "osteoarthritis knee joint." RESULTS AND CONCLUSION: Osteoarthritis is not yet a curable disease, and its pathogenesis remains unclear. The best treatment for osteoarthritis of the knee is prevention. The goal of therapy is to alleviate clinical manifestations. The therapeutic spectrum ranges from physiotherapy and orthopedic aids to pharmacotherapy and surgery.

Mid-range outcomes in 64 consecutive cases of multilevel fusion for degenerative diseases of the lumbar spine.

In the treatment of multilevel degenerative disorders of the lumbar spine, spondylodesis plays a controversial role. Most patients can be treated conservatively with success. Multilevel lumbar fusion with instrumentation is associated with severe complications like failed back surgery syndrome, implant failure, and adjacent segment disease (ASD). This retrospective study examines the records of 70 elderly patients with degenerative changes or instability of the lumbar spine treated between 2002 and 2007 with spondylodesis of more than two segments. Sixty-four patients were included; 5 patients had died and one patient was lost to follow-up. We evaluated complications, clinical/radiological outcomes, and success of fusion. Flexion-extension and standing X-rays in two planes, MRI, and/or CT scans were obtained pre-operatively. Patients were assessed clinically using the Oswestry disability index (ODI) and a Visual Analogue Scale (VAS). Surgery performed was dorsolateral fusion (46.9%) or dorsal fusion with anterior lumbar interbody fusion (ALIF; 53.1%). Additional decompression was carried out in 37.5% of patients. Mean follow-up was 29.4±5.4 months. Average patient age was 64.7±4.3 years. Clinical outcomes were not satisfactory for all patients. VAS scores improved from 8.6±1.3 to 5.6±3.0 pre- to post-operatively, without statistical significance. ODI was also not significantly improved (56.1±22.3 pre- and 45.1±26.4 post-operatively). Successful fusion, defined as adequate bone mass with trabeculation at the facets and transverse processes or in the intervertebral segments, did not correlate with good clinical outcomes. Thirty-five of 64 patients (54%) showed signs of pedicle screw loosening, especially of the screws at S1. However, only 7 of these 35 (20%) complained of corresponding back pain. Revision surgery was required in 24 of 64 patients (38%). Of these, indications were adjacent segment disease (16 cases), pedicle screw loosening (7 cases), and infection (one case). At follow-up of 29.4 months, patients with radiographic ASD had worse ODI scores than patients without (54.7 vs. 36.6; P<0.001). Multilevel fusion for degenerative disease still has a high rate of complications, up to 50%. The problem of adjacent segment disease after fusion surgery has not yet been solved. This study underscores the need for strict indication guidelines to perform lumbar spine fusion of more than two levels.

Functional midterm outcome in 131 consecutive cases of surgical clubfoot treatment.

INTRODUCTION: Since introduction and widespread use of the Ponseti method in the last decade, the need for surgical treatment of clubfeet is nowadays limited to resistant cases. In the time before, surgery via dorsomedial release was a very common treatment option. METHOD: Very few long-term follow-up studies cover the outcome of surgical methods, which is particularly interesting, as clinical results rather worsen with time. In the present study, 98 children (131 clubfeet), who underwent surgical correction using the Imhauser method at the age of 4.5 months were included. Follow-up time was 8.2 years (0-11.8 years) at average. RESULTS: Data could be retrieved from 46 patients (71 feet), only 5 patients (7 feet) were lost to follow-up. The rate of relapses was high, as 47 patients (53 feet) needed surgical revision for recurrence, while clinical scores showed a good result in the Laaveg and Ponseti Score and the Foot-Function-Index. Isokinetic testing and clinical data indicated a significant weakness of the treated foot, when compared to the healthy side in 12 patients with unilateral deformity. The presented study supports like others the issue that the clinical outcome of a surgical, posteromedial release in terms of relapses is disappointing. This fact is apparently not sufficiently reflected in the current clinical scores, which showed rather good results. CONCLUSION: As the question of evaluation methods for results of clubfoot treatment remains controversial, isokinetic testing is an easy to use alternative that provides detailed information about functional limitations and may help in reducing the need for repeated radiographic examinations.

Schwann cells overexpressing FGF-2 alone or combined with manual stimulation do not promote functional recovery after facial nerve injury.

PURPOSE: To determine whether transplantation of Schwann cells (SCs) overexpressing different isoforms of fibroblast growth factor 2 (FGF-2) combined with manual stimulation (MS) of vibrissal muscles improves recovery after facial nerve transection in adult rat. PROCEDURES: Transected facial nerves were entubulated with collagen alone or collagen plus naïve SCs or transfected SCs. Half of the rats received daily MS. Collateral branching was quantified from motoneuron counts after retrograde labeling from 3 facial nerve branches. Quality assessment of endplate reinnervation was combined with video-based vibrissal function analysis. RESULTS: There was no difference in the extent of collateral axonal branching. The proportion of polyinnervated motor endplates for either naïve SCs or FGF-2 over-expressing SCs was identical. Postoperative MS also failed to improve recovery. CONCLUSIONS: Neither FGF-2 isoform changed the extent of collateral branching or polyinnervation of motor endplates; furthermore, this motoneuron response could not be overridden by MS.

Electrical stimulation of paralyzed vibrissal muscles reduces endplate reinnervation and does not promote motor recovery after facial nerve repair in rats.

The outcome of peripheral nerve injuries requiring surgical repair is poor. Recent work has suggested that electrical stimulation (ES) of denervated muscles could be beneficial. Here we tested whether ES has a positive influence on functional recovery after injury and surgical repair of the facial nerve. Outcomes at 2 months were compared to animals receiving sham stimulation (SS). Starting on the first day after end-to-end suture (facial-facial anastomosis), electrical stimulation (square 0.1 ms pulses at 5 Hz at an ex tempore established threshold amplitude of between 3.0 and 5.0V) was delivered to the vibrissal muscles for 5 min a day, 3 times a week. Restoration of vibrissal motor performance following ES or SS was evaluated using the video-based motion analysis and correlated with the degree of collateral axonal branching at the lesion site, the number of motor endplates in the target musculature and the quality of their reinnervation, i.e. the degree of mono- versus poly-innervation. Neither protocol reduced collateral branching. ES did not improve functional outcome, but rather reduced the number of innervated motor endplates to approximately one-fifth of normal values and failed to reduce the proportion of poly-innervated motor endplates. We conclude that ES is not beneficial for recovery of whisker function after facial nerve repair in rats.

Manual stimulation of the orbicularis oculi muscle improves eyelid closure after facial nerve injury in adult rats.

We have shown that manual stimulation of rat whisker-pad muscles following facial-facial-anastomosis (FFA) restores normal whisking by lowering the proportion of polyinnervated motor endplates. Here we examined whether manual stimulation of the orbicularis oculi muscle (OOM) after FFA would also improve outcome. Blink responses to standardized air puffs were analyzed using video-based motion analysis. Two months after FFA, blink capacity was impaired, as indicated by a largely increased minimum distance between the eyelids after air-puff stimulation compared with intact rats (2.7 +/- 0.4 vs. 0.2 +/- 0.01 mm). Manual stimulation reduced this deficit by a factor of two (1.3 +/- 0.5 mm). The functional improvement after manual stimulation was associated with a 2-fold decrease in the proportion of polyinnervated OOM endplates (21 +/- 10% vs. 42 +/- 10% without manual stimulation, 0% in intact rats). We conclude that manual stimulation is a noninvasive and simple procedure with immediate potential for clinical rehabilitation of eyelid closure following facial nerve injury.

Treatment delay of bone tumours, compilation of a sociodemographic risk profile: a retrospective study.

BACKGROUND: Bone tumours are comparatively rare tumours and delays in diagnosis and treatment are common. The purpose of this study was to analyse sociodemographic risk factors for bone tumour patients in order to identify those at risk of prolonged patients delay (time span from first symptoms to consultation), professional delay (from consultation to treatment) or symptom interval (from first symptoms to treatment). Understanding these relationships might enable us to shorten time to diagnosis and therapy. METHODS: We carried out a retrospective analysis of 265 patients with bone tumours documenting sociodemographic factors, patient delay, professional delay and symptom interval. A multivariate explorative Cox model was performed for each delay. RESULTS: Female gender was associated with a prolonged patient delay. Age under 30 years and rural living predisposes to a prolonged professional delay and symptom interval. CONCLUSION: Early diagnosis and prompt treatment are required for successful management of most bone tumour patients. We succeeded in identifying the histology independent risk factors of age under 30 years and rural habitation for treatment delay in bone tumour patients. Knowing about the existence of these risk groups age under 30 years and female gender could help the physician to diagnose bone tumours earlier. The causes for the treatment delays of patients living in a rural area have to be investigated further. If the delay initiates in the lower education of rural general physicians, further training about bone tumours might advance early detection. Hence the outcome of patients with bone tumours could be improved.

Stabilization of the femoral head with a gliding screw in slipped capital femoral epiphysis.

OBJECTIVE: In situ fixation of the proximal femoral epiphysis to prevent further dislocation while maintaining the potential for longitudinal growth by insertion of a central gliding screw. Prevention of secondary coxarthrosis. INDICATIONS: Incipient and imminent slipped capital femoral epiphysis in children with a displacement angle of < 30 degrees in the axial view (ET' < 30 degrees ) and prophylactic treatment of the contralateral side. CONTRAINDICATIONS: Allergies to implant materials. SURGICAL TECHNIQUE: A Kirschner wire is inserted through a lateral proximal approach in the femur into the center of the displaced epiphysis at a right angle to its base. Overdrilling of the wire, thread tapping in the cancellous bone, insertion of the cannulated gliding screw with washer. The screw threads lie only in the epiphysis. The unthreaded part of the screw bridges the growth plate. The screw is allowed to protrude by about 2.5 cm to prevent an epiphyseodesis effect. POSTOPERATIVE MANAGEMENT: Partial weight bearing of the affected extremity up to 10 kg body weight for 6 weeks. Thrombosis prophylaxis during convalescence only for adolescents with signs of puberty (menarche/pubarche). Restriction on sports activities for 3 months. Follow-ups: clinical and radiologic examinations at 3 months postoperatively, then clinical examination every 6 months and radiologic assessment annually (possibly earlier, if there is a growth spurt). The gliding screw is removed when growth is completed. RESULTS: 63 hips (34 prophylactically and 29 therapeutically) were stabilized with the gliding screw. Clinically, the prophylactically stabilized hips showed excellent outcomes; of the therapeutically stabilized hips, the results were 19 excellent, seven good, two satisfactory, and one poor. Differences in leg length were seen in five cases, whereby the average value was 0.3 cm (0-1 cm). Chondrolysis or femoral head necrosis was not observed. Screw revision was necessary in 22 cases because contact between the screw head and the cortex would have prevented continued longitudinal growth (unintentional epiphyseodesis).