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1.
J Glaucoma ; 27 Suppl 1: S87-S90, 2018 07.
Article in English | MEDLINE | ID: mdl-29965902

ABSTRACT

The treatment of glaucoma in exfoliation syndrome is similar to primary open-angle glaucoma. Frequently, exfoliation glaucoma (XFG) patients require early polytherapy with topical medications. Little emphasis has been placed on tailoring treatment specifically to XFG. New outflow enhancing agents with novel mechanisms of action, such as Rho Kinase inhibition, NO signaling (both recently FDA-approved drugs) and adenosine α1-receptor stimulation, act directly on the trabecular meshwork. These agents may prove to be effective in lowering intraocular pressure and perhaps altering the pathogenesis of XFG aid in the long-term management of this disease.


Subject(s)
Antihypertensive Agents/therapeutic use , Exfoliation Syndrome/drug therapy , Glaucoma, Open-Angle/drug therapy , Adrenergic alpha-2 Receptor Agonists/therapeutic use , Adrenergic beta-Antagonists/therapeutic use , Carbonic Anhydrase Inhibitors/therapeutic use , Cholinergic Agents/therapeutic use , Humans , Intraocular Pressure/drug effects , Prostaglandins, Synthetic/therapeutic use , Tonometry, Ocular
2.
J Chromatogr A ; 1304: 1-9, 2013 Aug 23.
Article in English | MEDLINE | ID: mdl-23876769

ABSTRACT

A new approach based on microextraction by packed sorbent (MEPS) and a reversed-phase ultra-high pressure liquid chromatography (UHPLC) method was developed and validated for the determination and quantification of nonsteroidal anti-inflammatory drugs (NSAIDs) (acetylsalicylic acid, ketoprofen, diclofenac, naproxen and ibuprofen) in human urine. The important factors that could influence the extraction were previously screened using the Plackett-Burman design approach. The optimal MEPS extraction conditions were obtained using C18 phase as a sorbent, small sample volume (20µL) and a short time period (approximately 5min) for the entire sample preparation step. The analytes were separated on a core-shell column (Poroshell 120 EC-C18; 100mm×3.0mm; 2.7µm) using a binary mobile phase composed of aqueous 0.1% trifluoroacetic acid and acetonitrile in the gradient elution mode (4.5min of analysis time). The analytical method was fully validated based on linearity, limits of detection (LOD), limits of quantification (LOQ), inter- and intra-day precision and accuracy, and extraction yield. Under optimised conditions, excellent linearity (R(2)>0.9991), limits of detection (1.07-16.2ngmL(-1)) and precision (0.503-9.15% RSD) were observed for the target drugs. The average absolute recoveries of the analysed compounds extracted from the urine samples were 89.4-107%. The proposed method was also applied to the analysis of NSAIDs in human urine. The new approach offers an attractive alternative for the analysis of selected drugs from urine samples, providing several advantages including fewer sample preparation steps, faster sample throughput and ease of performance compared to traditional methodologies.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/urine , Chromatography, High Pressure Liquid/methods , Solid Phase Microextraction/methods , Chromatography, Reverse-Phase/methods , Humans , Limit of Detection
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