ABSTRACT
Candida fukuyamaensis RCL-3 yeast has the ability to decrease copper concentration in a culture medium. High copper concentrations change the cell color from white/cream to brown. The effect of color change ceases with the addition of KCN or when cells are grown in a culture medium without sulfate ions. These results could be associated with CuS bioaccumulation in the cell surface. This report revealed that mineralization would be a mechanism used by this yeast for copper bioremediation.
Subject(s)
Candida/metabolism , Copper/metabolism , Biodegradation, Environmental , Biotransformation , Candida/drug effects , Color , Copper Sulfate/metabolism , Crystallization , Culture Media/metabolism , Potassium Cyanide/pharmacology , Sulfates/pharmacologyABSTRACT
OBJECTIVE: This survey compares certain quality of work-life factors between a relocated work group and a control group. METHODS: A self-administered, cross-sectional survey was conducted 12 months after five departments (304 workers) had been relocated between two public health sites. The survey explored the workers' psychosocial job characteristics, their perceived health, and psycho-organizational constraints. The results compared both the relocated and control groups by using univariate and then multivariate statistical analyzes. RESULTS: When compared with the control group (nâ=â272), the relocated group (nâ=â180) showed a higher prevalence of psychosocial job characteristic "isostrain." The relocated group showed greater psycho-organizational constraints regarding the organizations favoring communication and team relationships. CONCLUSIONS: It seems that staff relocation can provoke a sense of uncertainty and isolation. Perhaps better communication might have reduced this and deter possible negative health outcomes.
Subject(s)
Health Personnel/psychology , Hospital Departments/organization & administration , Organizational Innovation , Stress, Psychological/epidemiology , Workplace/organization & administration , Workplace/psychology , Adult , Cross-Sectional Studies , Female , Health Status , Hospitals, University/organization & administration , Humans , Male , Middle Aged , Prevalence , Quality of Life , Surveys and Questionnaires , UncertaintyABSTRACT
Candida fukuyamaensis RCL-3 yeast strain isolated from a copper filter plant is able to lower copper concentration in culture medium. In the present study, effect of copper in proteins expression and mechanisms involved in copper resistance were explored using comparative proteomics. Mono-dimensional gel electrophoresis revealed differential band expressions between cells grown with or without copper. 2-DE analysis of C. fukuyamaensis RCL-3 revealed that copper exposure produced at least an over-expression of 40 proteins. Sixteen proteins were identified and grouped in four categories according to their functions: glycolysis and ATP production, synthesis of proteins, oxidative stress response, and processing and transport of proteins. Integral membrane proteins and membrane-associated proteins were analyzed, showing nine protein bands over-expressed in Cu-supplemented medium. Four proteins were identified, namely nucleoporin pom152, elongation factor 2, copper chaperone Sod1 Ccs1, and eiosome component Lsp1. The proteomic analysis performed allowed the identification of different metabolic pathways and certain proteins involved in metal input and storage related to cell ability to bioremediate copper. These proteins and mechanisms could be used for future applications of C. fukuyamaensis RCL-3 in biotechnological processes such as remediation of heavy metals.
