ABSTRACT
Nocardial conjunctivitis associated with silicone tubing is an extremely rare finding. The authors present a case of a 52-year-old woman with previous dacryocystorhinostomy and silicone tube placement 3 years prior who presented with OD redness and discharge for 1 week. On examination, the patient was noted to have mucoid discharge and crusting surrounding the silicone tube. The tube debris was sampled, and the culture was positive for Nocardia nova complex sensitive to trimethoprim/sulfamethoxazole and amikacin. Silicone tube colonization and N. nova complex conjunctivitis are both rare but should be considered in the differential diagnosis of patients with indwelling silicone tubes presenting with chronic conjunctivitis resistant to fluoroquinolones and tobramycin.
Subject(s)
Conjunctivitis, Bacterial/microbiology , Eye Infections, Bacterial/microbiology , Nocardia Infections/microbiology , Nocardia/isolation & purification , Prosthesis-Related Infections/microbiology , Silicone Elastomers , Stents/microbiology , Anti-Bacterial Agents/therapeutic use , Chronic Disease , Conjunctivitis, Bacterial/diagnosis , Conjunctivitis, Bacterial/drug therapy , Dacryocystorhinostomy/instrumentation , Eye Infections, Bacterial/diagnosis , Eye Infections, Bacterial/drug therapy , Female , Humans , Intubation/instrumentation , Microbial Sensitivity Tests , Middle Aged , Nocardia Infections/diagnosis , Nocardia Infections/drug therapy , Prosthesis-Related Infections/diagnosis , Prosthesis-Related Infections/drug therapy , Trimethoprim, Sulfamethoxazole Drug Combination/therapeutic useABSTRACT
Preparation of a random-sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer or via commercial synthesis companies. Purification of a single-stranded pool and conversion to a double-stranded pool are presented as step-by-step protocols. Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions.
Subject(s)
DNA/chemistry , Base Sequence , DNA, Single-Stranded/chemical synthesis , Molecular Biology/methodsABSTRACT
Preparation of a random-sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer or via commercial synthesis companies. Purification of a single-stranded pool and conversion to a double-stranded pool are presented as step-by-step protocols. Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions.
