Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Arch Physiol Biochem ; 113(1): 13-24, 2007 Feb.
Article in English | MEDLINE | ID: mdl-17522981

ABSTRACT

There seems to be an association between increased concentrations of malonyl coenzyme A (malonyl CoA) in skeletal muscle and diabetes and/or insulin resistance. The purpose of the current study was to test the hypothesis that treatments designed to manipulate malonyl CoA concentrations would affect insulin-stimulated glucose transport in cultured C2C12 myotubes. We assessed glucose transport after polyamine-mediated delivery of malonyl CoA to myotubes, after incubation with dichloroacetate (which reportedly increases malonyl CoA levels), or after exposure of myotubes to 2-bromopalmitate, a carnitine palmitoyl transferase I inhibitor. All three of these treatments prevented stimulation of glucose transport by insulin. We also assayed glucose transport after 30 min of inhibition of acetyl coenzyme A carboxylase (ACC), the enzyme which catalyzes the production of malonyl CoA. Three unrelated ACC inhibitors (diclofop, clethodim, and Pfizer CP-640186) all enhanced insulin-stimulated glucose transport. However, none of the treatments designed to manipulate malonyl CoA concentrations altered markers of proximal insulin signaling through Akt. The findings support the hypothesis that acute changes in malonyl CoA concentrations affect insulin action in muscle cells but suggest that the effects do not involve alterations in proximal insulin signaling.


Subject(s)
Glucose/metabolism , Insulin/physiology , Malonyl Coenzyme A/physiology , Muscle Fibers, Skeletal/enzymology , Animals , Biological Transport, Active/drug effects , Biological Transport, Active/physiology , Cattle , Cell Line , Dichloroacetic Acid/pharmacology , Mice , Muscle Fibers, Skeletal/drug effects , Muscle Fibers, Skeletal/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...