Acoustic Monitoring Confirms Significant Poaching Pressure of European Turtle Doves (Streptopelia turtur) during Spring Migration across the Ionian Islands, Greece.

The European turtle dove (Streptopelia turtur) is an Afro-Palearctic migrant whose populations have declined by 79% from 1980 to 2014. In 2018, the International Single Species Action Plan for the Turtle Dove (ISSAP) was developed with the goal of enabling, by 2028, an increase in turtle dove numbers along each of the three migration flyways (western, central, eastern). To achieve this, the illegal killing of turtle doves, a critical threat to the species, has to be eradicated. The Ionian Islands off the west coast of Greece lie on the eastern flyway and are considered a major turtle dove poaching hot-spot during spring migration. Quantifying wildlife crime, however, is challenging. In the absence of a reliable protocol for monitoring spring poaching levels, the agencies tasked with tackling the problem have no means of assessing the effectiveness of the anti-poaching measures and adapting them if required. Using passive acoustic monitoring (PAM) methods, we recorded gun hunting intensity at known turtle dove poaching sites during the 2019-2022 spring migrations (2-10 sites/season) with unprecedented spatial and temporal resolution. Based on published gunshot to killed/injured bird ratio for similar species (corroborated with discussions with local hunters) and an estimate of the proportion of hunting sites monitored by our PAM grid (using gunshot detection range estimates from control gunshots), we estimated that in 2021, up to 57,095 turtle doves were killed or injured across five Ionian Islands (Zakynthos, Paxi, Antipaxi, Othoni, and Mathraki). The 2022 estimate was almost half, but it is unclear as to whether the change is due to a decline in poachers or turtle doves. We propose ways of improving confidence in future estimates, and call for a temporary moratorium of autumn turtle dove hunting in Greece-as per ISSAP recommendation-until spring poaching is eradicated and the eastern flyway population shows signs of a full recovery. Finally, we hope our findings will pave the way for the development of PAM grids at turtle dove poaching hot-spots across all migration flyways, contributing to the global conservation of the species.

Spatial Ecology and Diel Activity of European Wildcat (Felis silvestris) in a Protected Lowland Area in Northern Greece.

The Balkan populations of the European wildcat are among the least studied. This study reports the first findings on the spatial ecology and activity pattern of the wildcat in Greece and compares them to those of better studied northern populations. We fitted five wildcats (two males, three females) with collars containing GPS and accelerometer loggers (E-obs 1A) and collected data from fall to early summer. All animals moved within a mosaic of lowland agricultural fields, woodland patches, riparian forests and wetlands near the banks of a lake. The trapping rate was the highest reported for the species. The home range sizes, estimated using Brownian bridge movement models, ranged from 0.94 to 3.08 km2 for females and from 1.22 to 4.43 km2 for males. Based on overall dynamic body acceleration (ODBA) values estimated from the accelerometer data, the diel activity of male wildcats followed the species' typical nocturnal pattern with crepuscular peaks. Female activity varied seasonally, at times being cathemeral. We found only weak effects of environmental variables on wildcat activity, and no significant difference in the activity in open versus forested areas. Our findings suggest that human modified landscapes can play a significant role in the conservation of this typically forest-associated species.

Endoparasites of European Wildcats (Felis silvestris) in Greece.

The European wildcat (Felis silvestris) is the only wild felid living in Greece. Wildcat populations are declining due to anthropogenic and phenological unfavourable conditions, and parasites may have an additional negative impact. In the present study, the occurrence of endoparasites in wildcats in Greece and the potential threats posed to wildcats, domestic animals, and humans in the study areas has been investigated. In a six-year period, 23 road-killed wildcats and 62 wildcat faecal samples were collected from different areas of the country. Necropsy for the detection of endoparasites and standard parasitological examinations of faecal samples were performed. Parasites were morphologically identified and, in selected cases, molecularly analysed. All necropsied wildcats (100%) were infected by three to 10 different parasite taxa, with the most prevalent being Taenia taeniaeformis (73.9%), Toxocara cati (60.9%), Angiostrongylus chabaudi (56.5%), Ancylostoma tubaeforme (39.1%), Cylicospirura spp. (34.8%), Troglostrongylus brevior (34.8%), and Capillaria aerophila (33.8%). Of the 62 faecal samples examined, 53 (85.5%) were positive for one or more parasite elements (larvae, eggs, or oocysts). The most frequent were T. cati (45.2%), A. chabaudi (29%), C. aerophila (24.2%), and Ancylostomatidae (17.7%). This is the first survey on endoparasites affecting wildcats in Greece. Some of the parasites here found are frequent in domestic and wild felids, while others, i.e., Oslerus rostratus and Cylicospirura petrowi, were described for the first time in the European wildcat. Most of them have a significant pathogenic potential, causing severe to hazardous diseases to infected felids and some, under specific circumstances, can also threaten human health.

Clinical investigations and treatment outcome in a European wildcat (Felis silvestris silvestris) infected by cardio-pulmonary nematodes.

Recently, feline cardio-pulmonary nematodes have attracted high scientific interest, as they are increasingly reported from various areas. Most of these parasites have similar transmission patterns and/or host reservoirs, thus they may affect domestic and wild felids living in sympatry. In the present study, a case of multiple cardio-pulmonary parasitism in co-infection with other parasites in a European wildcat is presented. The animal, found exhausted, was hospitalised for recovery and parasitological, haematological, clinical and imaging examinations were performed. The parasitological examinations revealed 4 cardio-pulmonary nematodes, i.e. Aelurostrongylus abstrusus, Troglostrongylus brevior, Eucoleus aerophilus, Angiostrongylus chabaudi, 3 intestinal parasites, i.e. Toxocara cati, ancylostomatids, Cystoisospora felis, 2 haemoparasites, i.e. Hepatozoon felis and elements morphologically compatible with small Babesia/Cytauxzoon spp., and Ixodes ricinus and Haemaphysalis erinacei ticks. Treatment with a spot-on formulation containing imidacloprid 10% and moxidectin 1% (Advocate® spot-on solution for cats, Bayer) was decided and follow-up faecal examinations were performed until the release of the animal. By the end of the hospitalisation, all metazoan endoparasites were no longer detectable in faecal examinations, with the exception of a low number of A. abstrusus larvae. Thus, the animal was released after a second treatment with the same product. This is the first description of an apparently successful treatment of multiple cardio-respiratory parasitosis in a naturally infected wildcat showing compatible clinical signs. The evidence that Advocate® may be effective against A. chabaudi could be useful for treating infected, hospitalised, wildcats and it is promising in the case A. chabaudi infection will spread to domestic cats in a near future.

First evidence of the European wildcat (Felis silvestris silvestris) as definitive host of Angiostrongylus chabaudi.

Angiostrongylus chabaudi (Strongylida, Angiostrongylidae) is a parasitic nematode described for the first time last century from the pulmonary arteries of six European wildcats (Felis silvestris silvestris) in central Italy. Since then, this parasite remained practically unknown until recently, when immature A. chabaudi have been reported from one wildcat in Germany and two domestic cats (Felis silvestris catus) in Italy. The present report describes the first record of A. chabaudi in Greece and, most importantly, the first known case of patent infection by A. chabaudi. The necropsy of a road-killed F. s. silvestris found near the lake Kerkini, in the municipality of Serres (Macedonia, Greece), revealed the presence of nematodes of both sexes in the right ventricle and the pulmonary artery of the heart. All parasites were mature adults and numerous eggs were present in the uteruses of females. The morphological characteristics of the parasites were consistent with those of A. chabaudi. Moreover, Angiostrongylus-like first stage larvae (L1) were present in the faeces of the animal that was negative for any other cardio-pulmonary parasite. Genetic examination of adult parasites and L1 confirmed the morphological identification as A. chabaudi. Histopathological examination of the lungs showed severe, multifocal to coalescing, chronic, interstitial granulomatous pneumonia due to the presence of adult parasites, larvae and eggs. These findings demonstrate for the first unequivocal time that this nematode reproduces in the European wildcat which should be ultimately considered a definitive host of A. chabaudi. Finally, the L1 of A. chabaudi are described here for the first time, opening new prospects for further studies on this neglected parasite.