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1.
Stem Cell Reports ; 18(4): 899-914, 2023 04 11.
Article in English | MEDLINE | ID: mdl-36963389

ABSTRACT

Cell replacement therapy is expected as a new and more radical treatment against brain damage. We previously reported that transplanted human cerebral organoids extend their axons along the corticospinal tract in rodent brains. The axons reached the spinal cord but were still sparse. Therefore, this study optimized the host brain environment by the adeno-associated virus (AAV)-mediated expression of axon guidance proteins in mouse brain. Among netrin-1, SEMA3, and L1CAM, only L1CAM significantly promoted the axonal extension of mouse embryonic brain tissue-derived grafts. L1CAM was also expressed by donor neurons, and this promotion was exerted in a haptotactic manner by their homophilic binding. Primary cortical neurons cocultured on L1CAM-expressing HEK-293 cells supported this mechanism. These results suggest that optimizing the host environment by the AAV-mediated expression of axon guidance molecules enhances the effect of cell replacement therapy.


Subject(s)
Neural Cell Adhesion Molecule L1 , Animals , Mice , Humans , Neural Cell Adhesion Molecule L1/metabolism , Neural Cell Adhesion Molecule L1/pharmacology , HEK293 Cells , Axons/metabolism , Pyramidal Tracts , Brain/metabolism , Netrin-1/metabolism , Netrin-1/pharmacology
2.
Cell Transplant ; 24(10): 1931-43, 2015.
Article in English | MEDLINE | ID: mdl-25396326

ABSTRACT

Patients with severe hypophosphatasia (HPP) develop osteogenic impairment with extremely low alkaline phosphatase (ALP) activity, resulting in a fatal course during infancy. Mesenchymal stem cells (MSCs) differentiate into various mesenchymal lineages, including bone and cartilage. The efficacy of allogeneic hematopoietic stem cell transplantation for congenital skeletal and storage disorders is limited, and therefore we focused on MSCs for the treatment of HPP. To determine the effect of MSCs on osteogenesis, we performed multiple infusions of ex vivo expanded allogeneic MSCs for two patients with severe HPP who had undergone bone marrow transplantation (BMT) from asymptomatic relatives harboring the heterozygous mutation. There were improvements in not only bone mineralization but also muscle mass, respiratory function, and mental development, resulting in the patients being alive at the age of 3. After the infusion of MSCs, chimerism analysis of the mesenchymal cell fraction isolated from bone marrow in the patients demonstrated that donor-derived DNA sequences existed. Adverse events of BMT were tolerated, whereas those of MSC infusion did not occur. However, restoration of ALP activity was limited, and normal bony architecture could not be achieved. Our data suggest that multiple MSC infusions, following BMT, were effective and brought about clinical benefits for patients with lethal HPP. Allogeneic MSC-based therapy would be useful for patients with other congenital bone diseases and tissue disorders if the curative strategy to restore clinically normal features, including bony architecture, can be established.


Subject(s)
Bone Marrow Transplantation , Hypophosphatasia/therapy , Mesenchymal Stem Cell Transplantation , Mesenchymal Stem Cells/cytology , Osteogenesis/physiology , Bone Marrow Transplantation/methods , Cell Differentiation/physiology , Cells, Cultured , Humans , Infant , Male , Mesenchymal Stem Cell Transplantation/methods , Transplantation, Homologous/methods , Treatment Outcome
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