Can additional air supply enhance decomposition processes in sludge treatment reed beds?

This work was designed to investigate the influence of artificial aeration on the sludge decomposition process in mesocosm sludge treatment reed beds (STRBs). In addition to the typical STRB design, where ventilation is mainly provided by a drainage pipe, passive aeration via a "chimney" and active aeration via a blower were introduced. During the entire observation period of 1.5 years, O2 concentrations in the upper part of the filter were significantly higher in the artificially aerated beds. To determine decomposition rates, a study with decomposition bags, measurements of CO2 emissions from the STRB and isotopic partitioning of CO2 emissions were performed. The results indicate an accelerated sludge degradation process in both active and passive beds. However, this effect was limited to part of the season and could not be demonstrated by episodic measurements of CO2 efflux. The CO2 efflux showed a seasonal pattern. Average CO2 efflux was below 3.0 µmol m-2 s-1 in the winter months and reached 43 µmol m-2 s-1 in the warmer months. The low sludge load and drought period in summer 2018 resulted in an extremely low CO2 efflux in August 2018. Isotopic analyses revealed changes in decomposition dynamics for certain parts of the season, differences in contributions of sludge and plant derived CO2 to total CO2 emissions from differently aerated beds. Overall, passive aeration proved to be similarly efficient as active aeration and could therefore be considered for application in a full-scale system.

Genes controlling polyunsaturated fatty acid synthesis are developmentally regulated in broiler chicks.

1. The objective of this study was to characterise the regulation of the pathways that synthesise long-chain polyunsaturated fatty acids (PUFA) on developing adipose deposits in broiler embryos and chicks. Subcutaneous adipose depots were harvested from embryos and embryonic d E13, E15 and E17. Subcutaneous, abdominal and crop (neck) adipose, as well as liver, were collected at 7 and 14 d post-hatch. 2. Targeted RNA sequencing was used to quantify expression of 6 elongation of very long-chain fatty acid (ELOVL) genes, two isoforms of stearoyl-CoA desaturase (SCD and SCD5), and three fatty acid desaturases (FADS1, FADS2, and FADS6) in each depot and in the liver. Expression levels of marker genes for fatty acid oxidation and adipogenesis (peroxisome proliferator-activated receptor gamma (PPARG)) were quantified. Fatty acid composition of subcutaneous adipose was analysed using gas chromatograph-mass spectrometry (GC/MS). 3. Genes in the PUFA synthetic pathway were differentially expressed across developmental ages and between depots. These include elongase and desaturase genes, that have not previously been characterised in chicken. Correlation analyses identified subsets of co-regulated genes and fatty acids and highlighted relationships that may influence adipose metabolism and development. 4. It was concluded that PUFA synthesis is an active and dynamically regulated pathway in developing adipose deposits in the broiler chick. These data highlighted potential novel roles for specific elongase and desaturase genes in adipose deposition and metabolism.

Associations of the interleukin-1 gene locus polymorphisms with risk to hip and knee osteoarthritis: gender and subpopulation differences.

Genetic predisposition to the complex hereditary disease like osteoarthritis (OA) of the large joints (hip and knee) includes the interleukin-1 gene (IL-1) cluster on chromosome 2. Using a case-control study with 500 OA patients (240 knee and 260 hip OA patients, all with joint replacement), we analysed frequencies of IL-1 gene cluster polymorphisms in Croatian Caucasian population. The control samples came from 531 healthy individuals including blood donors. We genotyped two single nucleotide polymorphisms in the IL-1 gene locus at IL-1A (-889, C>T, rs1800587) and IL-1B (+3594, C>T, rs1143634) and compared their frequencies between patients and controls. We predicted haplotypes by combining current data with our previous results on gene polymorphisms (IL-1B, rs16944 and the IL-1 receptor antagonist gene [IL-1RN] variable number tandem repeat [VNTR]) for the same population. Haplotype analyses revealed gender disparities and showed that women carriers of the 1-2-1-1 haplotype [IL-1A(rs1800587) - IL-1B(rs1143634) - IL-1B(rs16944) - IL-1RN(VNTR)] had sixfold lower risk to develop knee OA. However, carriers of the 1-1-1-2 haplotype of both sexes had over twofold higher predisposition to hip OA. Our results differ from some earlier studies in Caucasian subpopulations, which may be due to the fact that this is the first study to separate genders in assessing the IL-1-locus genetic risk of OA. The results suggest that inflammatory mediators like IL-1 might be implicated in the pathogenesis of primary OA in large joints and that as yet unidentified gender-specific factors exist in a Croatian Caucasian population.

Maintenance therapy in multiple myeloma.

Therapeutic advances in the treatment of multiple myeloma have significantly improved remission duration and overall survival (OS). These strategies have included the use of immunotherapy (interferon), novel agents (bortezomib, thalidomide, and lenalidomide), corticosteroids, and chemotherapy. While novel agents have had a major impact on response rates with initial therapy, most patients with multiple myeloma will eventually relapse. In the setting of minimal residual disease following standard dose or high-dose therapy, a number of different 'maintenance' strategies have emerged to prolong the duration of initial or subsequent remissions. The impact of these strategies on OS and event-free survival (EFS) is critically important, as the use of ineffective maintenance therapy adds the burden of additional cost, morbidity, and may reduce quality of life. Truly successful maintenance therapy will be effective in the setting of minimal residual disease, and will improve not only EFS, but also OS. This review summarizes the currently available data in the maintenance setting for multiple myeloma, and will discuss potential future trials to further address this important issue.

[Ultrasonic diagnosis of abscesses in tubercular spondylitis of the vertebrae]. / Mogucnosti ultrazvucne dijagnostike kod apscesa tuberkuloznog spondilitisa kraljeznice.

Over the period of the last year morbidity of TBC spondylitis of vertebrae due to orthopaedic research in Special clinical orthopaedic hospital Lovran, is found to be increasing once again. In consideration of difficulties of citing TBC abscess of vertebrae in several cases, to help ourselves we have tried to solve this problem with ultrasound. In the last two years 15 patients with TBC spondylitis of vertebrae have been examined. From these, inflammation process was located in thoracal part of vertebrae at 7 patients, and at 8 patients in lumbar part. After result evolution, we have found out that abscesses located in abdominal part could have been cited with ultrasound. With the present degree of accoutrements abscesses in thoracal part located close to spine couldn't be cited by this method of research. The reason was absorption of ultrasound waves in bony structures of torax. By guidance of ultrasound it is possible to accomplish puncture of abscess's sack.