Subject(s)
Immunoelectrophoresis/methods , Immunoglobulins/analysis , Adolescent , Adult , Female , Humans , Male , Middle AgedSubject(s)
Amylases/metabolism , Creatinine/metabolism , Pancreatitis/diagnosis , Acute Disease , Adult , Aged , Clinical Enzyme Tests , Female , Humans , Male , Middle Aged , Pancreatitis/enzymologyABSTRACT
We describe a method of analysis for nickel in serum and urine. After the sample (1 ml) has been subjected to acid digestion, Ni(II) is extracted into methylisobutylketone as a furildioximate complex, and is measured by electrothermal atomic absorption spectrometry. The method is more sensitive and more convenient than previous methods, and is less subject to nickel contamination because the digestion and extraction steps are performed in a single tube. Analytical recovery of Ni(II) (10 or 20 microng/liter) added to 20 specimens of serum or urine averaged 101% (range, 94 to 107). The following values (mean +/- SD) were obtained for nickel concentrations in specimens of serum and urine from 19 healthy adults living in central Connecticut: serum = 3.1 +/- 1.6 microng/liter; urine = 2.7 +/- 1.6 microng/liter. The method provides a practical means for monitoring occupational exposures of workers to nickel compounds.
Subject(s)
Nickel/analysis , Oximes , Adult , Chelating Agents , Humans , Indicators and Reagents , Male , Nickel/blood , Nickel/urine , Quality Control , Spectrophotometry, Atomic/methodsABSTRACT
Investigations have been performed on the possible effect of zinc and copper on the fertility of man. Semen was obtained from 67 men and the zinc concentration was found to be decreasing with a drop of the activity of the semen. Copper was found in semen in microgram amounts which do not change with the activity of the semen. Results suggest that the zinc concentration may serve as evidence in the evaluation of semen activity.
