[THE CHARACTERISTIC OF SOMATOTYPE AND FUNCTIONAL STATE OF CIRCULATORY SYSTEM OF STUDENT YOUTH OF THE NORTHEAST OF RUSSIA].

The article considers results of single-step study in random sampling of female students of the M.K. Ammosov north-east federal university (n=456). The study was carried out to investigate somatotype and functional state of circulatory system. The standard technique was applied to measure height, body mass, chest circumference, level of arterial pressure and rate of heart beats. The type of somatotype was established using Pignet index. The tone of vegetative system was determined using Kérdö index. The adaptation potential of circulatory system was determined using functional changes index. The results of study established that in 61% of examined female students the type of constitution corresponds to normosthenic one. The percentage of persons with asthenic and hypersthenic type of constitution amounted to 27% and 12% correspondingly. The signs of increasing oftone ofsympathetic nervous system are observed in 89% of girls. The functional condition of circulatory system is evaluated as "tension of adaptation mechanisms" that is apparently related to period of adaptation to new conditions. The prolonged preservation of such states results in exhaustion offunctional resources of organism and can promote development of diseases. In conditions of impacting of unfavorable ecological factors the deconditionning disorders can significantly contribute to health disturbances and decreasing of life quality. To preserve youth's health during period of education the comprehensive strategy is to be implemented such components as dynamic monitoring of health, organization of adequate diet, explanation of necessity of observance of sleep and rest pattern, development of conditions for active aerobic physical exertion and activities on correction of risk factors of development of diseases are to be included.

[The evaluation of health condition of first year students].

The study was organized to evaluate initial health condition and to detect risk factors of chronic diseases in first year students of University. The sampling consisted of 649 students aged from 15 to 30 years (mean age is 18.8 ± 1.5 years). The analysis of total morbidity demonstrated that 46.1% of first year students suffered from chronic diseases. It is noted that diseases of musculo-skeletal system have especially high prevalence in students. The analysis of data of anonymous survey concerning behavioral risk factors established prevalence of tobacco smoking, alcohol consumption and sedentary life-style. Among students, 23.5% of males and 9.3% of females smoked and correspondingly 28.5% and 12.5% took alcoholic drinks. The physical activity of students was insufficient and only one third of respondents follows healthy life-style. The group of healthy students consisted only 18.2% and 81.8% had different deviations i.e. risk factors of development of chronic diseases.

[Vibrio cholerae 01, variant eltor, in the environment]. / Vibrio cholerae 01 biovara eltor v okruzhaiushchei srede.

The possibilities of the autochthonous existence of V. cholerae in open water reservoirs, depending on the combined effect of different biotic and abiotic factors are considered. The role of adaptive variability of V. cholerae O1, biovar eltor for its preservation in the environment is emphasized. The data on the duration of the V. cholerae O1 isolation from different environmental objects in some regions of Ukraine are presented.

[Factors contributing to preservation of Vibrio cholerae in water reservoirs]. / Faktory sokhraneniia kholernykh vibrionov v vodoemakh.

The summarized data of literature concerning the survival of V. cholerae in the environment and the influence of abiotic and biotic factors on this process are presented. These data make it possible to regard cholera as sapronosis and to form an idea of the role of factors contributing to the survival of V. cholerae in the environment and to its spread among human population.

[Polymorphism of membrane proteinases from mitochondria]. / Polimorfizm membrannyx proteinaz mitokhondrii.

Using native polyacrylamide gel electrophoresis in the absence of sodium dodecyl sulfate, the detergent extracts of sonic submitochondrial particles (SMP) were separated into three protein fractions capable of accomplishing the proteolysis of cytochrome c and three other fractions catalyzing the hydrolysis of N-a-benzoyl-L-arginine-p-nitroanilide (BAPA) and N-a-benzoyl-L-arginine-B-naphthylamide (BANA). The fractions isolated from the gel were subjected to a thorough anaylsis. Cytochrome c hydrolases were shown to have identical molecular weights (17000) but different isoelectric points (4.0, 4.2 and 4.4). The total cytochrome c hydrolase activity of these enzymes was inhibited by phenylmethylsulfonylfluoride but was insensitive to ethylenediaminetetraacetate and o-phenanthroline. Three BANA (BAPA) hydrolases have identical Mr values (approximately 17500) but different pI values (4.2, 4.3 and 4.7). Apart from the indicated hydrolases, the detergent extracts of SMP were shown to contain minor components with identical activities distinguished by the tightness of binding to the inner mitochondrial membrane, Mr and sensitivity to proteinase inhibitors. The observed phenomenon is considered to be due to the polymorphism of proteinases coupled with the inner mitochondrial membrane.

Detection, isolation and some properties of membrane proteinases from yeast mitochondria.

Three cytochrome c hydrolase species were found in the 0.05% SDS extract from submitochondrial particles. Their polypeptides all have a molecular weight of 17 000 but differ in pI values (4.0, 4.2 and 4.4), as shown by SDS-polyacrylamide gel electrophoresis and polyacrylamide gel isoelectric focusing. The activity of pooled cytochrome c hydrolases is sensitive to PMSF, pCMPS , and leupeptin but insensitive to EDTA or o-phenanthroline. Besides the cytochrome c-hydrolyzing enzymes, the SDS extract contains three protein components with BAPA ( BANA )-hydrolyzing activity, which also show similar molecular weights (17 5000) but different pI values (4.2, 4.3 and 4.7). It is supposed that at least some of the enzymes mentioned are involved in the intramembrane proteolysis of polypeptides synthesized on mitoribosomes .