[Modern methods in the serological diagnosis of toxoplasmosis]. / Sovremennye metody serologicheskoi diagnostiki toksoplazmoza.

A total of 235 sera from patients suspected for toxoplasmosis were examined in the enzyme immunoassay (EIA), complement fixation, indirect immunofluorescence (IIF) and indirect hemagglutination (IHA) tests carried out in parallel. Good correlation between all tests was noted. As confirmed by the data obtained in this investigation, EIA showed the highest sensitivity. In IIF and IHA tests the maximum titers (1:512) were obtained in 2% of cases, while in EIA 17.5% of sera were positive in titers of 1:800 to 1:25,000. High sensitivity and good reproducibility of this assay suggest the necessity for its standardization and automation, which will permit its further use in laboratories at any level.

[Comparative evaluation of the commercial Sevatest ELISA immunoenzyme set and other serological tests for detecting Toxoplasma gondii antibodies]. / Sravnitel'naia otsenka kommercheskogo immunofermentnogo nabora Sevatest ELISA i drugikh serologicheskikh testov dlia obnaruzheniia antitel k Toxoplasma gondii.

This work analyzes the results of 4 serologic tests used for the diagnosis of toxoplasmosis: the complement fixation (CFT), indirect immunofluorescence (IIF), passive hemagglutination (PHAT) tests, and the enzyme-linked immunosorbent assay (ELISA). The last-mentioned one was made with the use of the commercial kits Sevatest ELISA IgG/Toxo Micro I. The results of ELISA were in good correlation with those yielded by the traditional tests: 70% coincidence with CFT, 80% with IIF, 84% with PHAT; besides, ELISA has shown a higher sensitivity in the screening of sera.