[Antimutagenic hypoxen activity in vivo]. / Izuchenie antimutagennoi aktivnosti gipoksena in vivo.

The antimutagen effect of the drug hypoxen, representing poly(2,5-dihydroxyphenylene)-4-thiosulfonic acid sodium salt, was studied by chromosome aberration assay in the bone marrow cells of C57BL/6 mice. Hypoxen (20 mg/kg, i.p.) administered simultaneously with dioxidine (300 mg/kg, i.p.) reduced genotoxicity of the latter compound by 35% over a time period of 24 h. Preliminary five-day administration of hypoxen (70 mg/kg, p.o.) did not decrease the dioxidine damage. The genoprotector activity of hypoxen upon interaperitoneal injection is more pronounced as compared to that of the reference drug sladex (aspartam).

[Origin of the phagocytes of the central nervous system]. / O proiskhozhdenii fagotsitov TsNS.

Presence of cells having hemopoietic origin in an intact tissue and in the lesion focus in the brain of xenogenic (a rat and mice) radiative chimeras has been studied in order to elucidate the sources of phagocytes in the CNS and their relation to the microglial elements. To identify the cells of the hemopoietic origin in the xenogenic radiative chimeras, an indirect immunofluorescent method has been applied using a specific antiserum against nonsoluble antigens of the rat bone marrow cells. In the intact cerebral tissue of the xenogenic radiative chimeras no cells of the donor type have been revealed during one year after irradiation and bone marrow transplantation. The cells having hemopoietic origin (macrophages, leucocytes) are revealed in the lesion locus (inflammatory granuloma) and in the surrounding cerebral tissue. A suggestion is made that phagocytes in the CNS lesion focus are of hematogenic origin and that there is not any histogenic connection between the microglia and the system of mononuclear phagocytes.

[Cells of hematopoietic origin in the connective tissue of various organs in xenogeneic murine radiation chimeras]. / Kletki krovetvornogo proiskhozhdeniia v soedinitel'noi tkani raznykh organov ksenogennykh myshinykh radiatsionnykh khimer.

The distribution of donor cells in the connective tissue of different organs of xenogeneic (rat in mouse) radiation chimaeras was studied under the normal conditions and upon its de novo formation. The donor cells were identified by means of indirect immunofluorescent method with the use of specific antiserum against surface antigens of the rat bone marrow cells. It was shown that macrophages of subcutaneous connective tissue, lung alveolar macrophages, Kuppfer's cells, macrophages of intestinal mucosa in the long living radiochimaeras were represented by the donor cells. The fibroblast-like cells of donor origin were found in the subcutaneous loose connective tissue, among the connective tissue elements of cross striated muscles, in the cardiac muscle, in the intestinal mucosa. The repopulation of Peyer's patches of the radiochimaeras by the donor cells was demonstrated. The macrophages of hemopoietic origin (donor origin) were found in the inflammatory granuloma in the brain cortex. The donor cells were found in the connective tissue of mammary glands following the hormonal stimulation. Differences were found with respect to binding of the antiserum used to cells of the capsule around the foreign body in different organs of xenogeneic chimaeras.