ABSTRACT
The possibility of long-term changes in the innate characteristics of the behavior of mice of different genotypes (C57Bl/6 and BALB/c) after active immunization with dopamine-BSA conjugates was studied. These experiments revealed significant differences in the effects of anti-dopamine antibody on behavioral responses in an open field and neurotransmitter contents of brain structures in mice of different lines. Immunization with the dopamine-protein conjugate led to increases in the functional activity of the corresponding brain receptors, to different extents in mice of different lines.
Subject(s)
Antibodies/pharmacology , Behavior, Animal/drug effects , Brain/drug effects , Dopamine/pharmacology , Neuroimmunomodulation , Animals , Antibodies/metabolism , Brain/metabolism , Corpus Striatum/drug effects , Corpus Striatum/metabolism , Dopamine/immunology , Dopamine/metabolism , Genotype , In Vitro Techniques , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Motor Cortex/drug effects , Motor Cortex/metabolism , Radioligand Assay , Receptors, Dopamine D2/metabolism , Serotonin/metabolism , Serum Albumin, Bovine/pharmacology , Somatosensory Cortex/drug effects , Somatosensory Cortex/metabolism , VaccinationABSTRACT
Plasma concentration of immune complexes and antibodies to serotonin and dopamine were higher in patients with Alzheimer's disease compared to mentally healthy volunteers of the same age. Plasma concentrations of antibodies to serotonin and dopamine decreased with the increase in the duration of this disease.
Subject(s)
Alzheimer Disease/immunology , Autoantibodies , Dopamine/pharmacology , Serotonin/pharmacology , Aged , Aged, 80 and over , Alzheimer Disease/metabolism , Antigen-Antibody Complex/blood , Cardiotonic Agents/pharmacology , Female , Humans , Neurotransmitter Agents/metabolism , Serotonin/immunologyABSTRACT
Immunization of BALB/c mice with glutamate-BSA conjugate reduced anxiety and improved passive avoidance retention, while in C57Bl/6 mice immunization disturbed passive avoidance retention, but had no effect on anxiety. Interstrain differences in the shuttle box behavior were found between control animals.
Subject(s)
Antibodies/metabolism , Behavior/physiology , Glutamic Acid/immunology , Immune System/physiology , Mice, Inbred BALB C , Mice, Inbred C57BL , Animals , Avoidance Learning , Glutamic Acid/metabolism , Immunization , Male , MiceABSTRACT
We studied the effects of active immunization with glutamate-protein conjugates on behavioral reactions of C57Bl/6 and BALB/c mice in the open-field test and on animal anxiety in the tail suspension test. Antiglutamate antibodies produced different behavioral changes in mice of these strains.
Subject(s)
Antibodies/immunology , Behavior, Animal , Glutamic Acid/immunology , Animals , Genotype , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Species SpecificityABSTRACT
Effects of active immunization with the serotonin-protein conjugate on open-field behavior, learning and retention of the conditioned passive avoidance response, neurotransmitter contents in brain structures, and functional activity of central serotonin receptors were studied on C57Bl/6 and BALB/c mice. Serotonin antibodies produced opposite effects on the studied parameters in genetically different mouse strains.
Subject(s)
Behavior, Animal , Brain/metabolism , Neuroimmunomodulation , Neurotransmitter Agents/metabolism , Receptors, Serotonin/metabolism , Serotonin/immunology , Serum Albumin, Bovine/immunology , Animals , Antibodies/metabolism , Avoidance Learning , Conditioning, Psychological , Humans , Immunization , Ligands , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Motor Activity , Protein Binding , Serotonin/metabolismABSTRACT
Dysregulation of neuroimmune connections is a primary or secondary pathogenic factor of some CNS diseases. Autoimmune aggression is typical of multiple sclerosis, Alzheimer's disease, and epilepsy, while dysregulation characterized by enhanced production of autoantibodies to neurotransmitters and activation of cell factors is characteristic of alcoholism and drug abuse. In experimental models of alcoholism and drug addiction, protective effects of antiserotonin antibodies are mediated by immune cells stimulated by these antibodies. These effects can be used in the therapy of various forms of neuroimmunopathology by the method of adoptive immunotherapy.
Subject(s)
Central Nervous System Diseases/immunology , Central Nervous System Diseases/pathology , Immunotherapy , Alzheimer Disease/immunology , Alzheimer Disease/pathology , Alzheimer Disease/therapy , Animals , Central Nervous System Diseases/therapy , Epilepsy/immunology , Epilepsy/pathology , Epilepsy/therapy , Humans , Multiple Sclerosis/immunology , Multiple Sclerosis/pathology , Multiple Sclerosis/therapy , Neuroimmunomodulation/immunology , Neuroimmunomodulation/physiologyABSTRACT
The effect of antiserotonin antibodies on basal electrocorticogram was studied in electrophysiological experiments on rats. Intracortical injection of 10 microg antiserotonin antibodies into the sensorimotor cortex induced epileptiform activity in this area. It is assumed that antiserotonin antibodies modulate activity of cortical neurons due to both binding serotonin molecules and interaction with serotonin receptors.
Subject(s)
Antibodies/metabolism , Motor Cortex/metabolism , Serotonin/immunology , Serotonin/physiology , Animals , Electrophysiology , Male , Neurons/metabolism , Protein Binding , Rats , Rats, WistarABSTRACT
Single i.p. doses of anti-dopamine antibodies were given to C57Bl/6 mice. This resulted in inhibition of motor activity in a large proportion of the animals in the "open field" test, which lasted five days. Hyperalgesia, detected 1.5 h and 1 day after doses of antibody, was replaced by analgesia on day 5. There was a sharp reduction in the levels of dopamine and its metabolites in the cerebral cortex at 1 and 5 days; the serotonin level was increased 1 day after doses of antibody, and was significantly decreased at 5 days. There was no effect on cells of the immune system. The possible mechanisms of the neurotropic action of these antibodies are discussed.
Subject(s)
Antibodies, Blocking/pharmacology , Behavior, Animal/physiology , Biogenic Monoamines/metabolism , Central Nervous System/metabolism , Dopamine/immunology , Pain/psychology , Animals , B-Lymphocytes/drug effects , B-Lymphocytes/immunology , Behavior, Animal/drug effects , Central Nervous System/physiology , Macrophages, Peritoneal/drug effects , Macrophages, Peritoneal/immunology , Male , Mice , Mice, Inbred C57BL , Motor Activity/drug effects , Motor Activity/physiology , Pain Measurement/drug effects , Phagocytosis/drug effects , T-Lymphocytes/drug effects , T-Lymphocytes/immunologyABSTRACT
Experiments on C57Bl/6 mice kept under conditions of free choice between alcohol and water for 4 and 12 months showed that adoptive transfer of splenocytes extracorporeally stimulated with arginine vasopressin suppressed alcohol intake.
