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1.
Photodiagnosis Photodyn Ther ; 7(2): 98-105, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20510304

ABSTRACT

BACKGROUND: Susceptibility of bacterial and fungal species to the photodynamic killing effects of various photosensitizing dyes has received increasing attention. In the oral cavity oral candidiasis is primarily caused by Candida albicans. Evidence suggests that Oropharyngeal Candidiasis, found frequently in patients with immunodeficiency, present with mixed Candida organisms and are more difficult to treat than those solely due to C. albicans. In the present study we demonstrate the ability to efficiently kill antifungal resistant isolates of Candida using Photofrin induced PDT. METHODS: Candida strains from the ATCC as well as fluconazole and amphotericin B resistant and sensitive isolates from adults with AIDS were grown cultures and grown under standard conditions. Photofrin was added to appropriate cultures as dictated by experimental design. Light was delivered to assigned cultures using a 630 nm laser source at a power density of 150 mW/cm(2), for appropriate time to deliver 45-135 J/cm(2). Colony forming assays were used to determine survival. RESULTS: After illumination cultures treated with Photofrin had significant reduction in colony forming ability at all light doses examined. Isolates from AIDS patients which had demonstrated antifungal resistance showed equivalent sensitivity to photodynamic killing as did control ATCC cultures of the same strain. CONCLUSION: This study demonstrates Photofrin induced PDT can eliminate Candida species with significant efficiency as revealed by colony forming ability. Further Candida isolates from AIDS patients that had demonstrated fluconazole and amphotericin B resistance were equally susceptible to photodynamic killing.


Subject(s)
AIDS-Related Opportunistic Infections , Candida/radiation effects , Candidiasis/radiotherapy , Dihematoporphyrin Ether/therapeutic use , Drug Resistance, Fungal/radiation effects , AIDS-Related Opportunistic Infections/complications , AIDS-Related Opportunistic Infections/microbiology , Adult , Candida/classification , Cells, Cultured , Disease Susceptibility , Humans , Photosensitizing Agents/therapeutic use
2.
J Periodontol ; 63(7): 611-7, 1992 Jul.
Article in English | MEDLINE | ID: mdl-1324302

ABSTRACT

The studies reported here describe the development, characterization, and initial application of latex agglutination assays for periodontal pathogens. Latex reagents were prepared by sensitization of latex microspheres with rabbit IgG antibodies to either Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, or Prevotella intermedia. The protein concentration utilized for sensitization and microsphere size were optimized. One reagent was prepared to A. actinomycetemcomitans and a second combination reagent was prepared by mixing latex sensitized with antibodies to P. gingivalis and latex sensitized with antibodies to P. intermedia. The sensitivity of both latex reagents in the traditional wet and a dried format was evaluated. In addition, sensitivity and specificity with homologous and heterologous bacterial suspensions were evaluated. The reagents were found to demonstrate both specificity and sensitivity. Initial studies with subgingival human plaque demonstrated the ability of these reagents to detect the specific organisms in plaque.


Subject(s)
Aggregatibacter actinomycetemcomitans/isolation & purification , Bacteroides/isolation & purification , Latex Fixation Tests/methods , Periodontal Diseases/microbiology , Porphyromonas gingivalis/isolation & purification , Aggregatibacter actinomycetemcomitans/classification , Animals , Bacteria/classification , Bacteria/isolation & purification , Bacteroides/classification , Dental Plaque/microbiology , Fluorescent Antibody Technique , Gingivitis/microbiology , Humans , Immune Sera , Indicators and Reagents , Microspheres , Periodontitis/microbiology , Porphyromonas gingivalis/classification , Rabbits
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