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1.
ACS Omega ; 8(48): 46236-46251, 2023 Dec 05.
Article in English | MEDLINE | ID: mdl-38075786

ABSTRACT

A diverse silver-lactoferrin (AgLTF) complex, comprising silver ions (Ag+) and silver nanoparticles, displayed a synergistic antibacterial effect while being almost five times more lethal than LTF alone. Gas chromatography-mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-in linear (LP) and reflectron (RP) positive modes-were used to comprehensively analyze metabolites and proteins profiles of bacteria (Staphylococcus aureus (SA), Pseudomonas aeruginosa (PA) and Enterococcus faecalis (EF)) treated using AgLTF complex versus exclusively Ag+. Although both agents resulted in similar metabolic shifts in bacteria, AgLTF significantly triggered the production of sulfides (related to bacterial stress resistance), ethanol, 2-butanol (indicating exhaustion of cell respiration), decanoic acid, and nonane (suggesting ongoing oxidative stress). Keto acids formation and fermentation pathways were enhanced by AgLTF and suppressed by Ag+. Furthermore, AgLTF appears to interact with proteins fraction of bacteria in a concentration-dependent manner. EF molecular profiles showed less changes between treated and untreated bacteria. On the other hand, SA and PA proteins and metabolic patterns were the most differentiated from untreated bacteria. In conclusion, our study may provide valuable insights regarding the molecular mechanisms involved in AgLTF antimicrobial action.

2.
Sci Rep ; 13(1): 8933, 2023 06 01.
Article in English | MEDLINE | ID: mdl-37264070

ABSTRACT

The synthesis of efficient and low-cost coatings for solid-phase microextraction attracted much attention. Conductive polymers are excellent candidates for this purpose due to the possibility of electropolymerization, which results in the reproducible synthesis of films. A plethora of studies reported in the literature concluded that modification of conductive polymers with innovative materials could lead to an increase in sensitivity toward specific analytes. In this work, the metal-organic framework-polypyrrole composite was electrodeposited in one step directly onto a stainless-steel substrate. The effect of synthesis parameters on extraction efficiency was investigated. The obtained PPy@ZIF-8 coating was subjected to physical-chemical characterization using electron microscopy and Fourier-transform IR spectroscopy. The main finding of the study was that the values of the limit of detection and intra- and inter-day reproducibility for analytes with different chemical structures were found to be lower as compared to pure polypyrrole coating. Furthermore, the obtained polypyrrole-MOF coating was applied for the collection of profiles of volatile organic compounds liberated by bacteria. Hence, the polypyrrole@ZIF-8 coating synthesized using a low-cost and facile approach presented in this study can be useful for the profiling of VOCs liberated by bacteria.


Subject(s)
Polymers , Pyrroles , Polymers/chemistry , Pyrroles/chemistry , Solid Phase Microextraction/methods , Reproducibility of Results , Bacteria
3.
Int J Mol Sci ; 23(13)2022 Jun 26.
Article in English | MEDLINE | ID: mdl-35806114

ABSTRACT

Antibiotic-resistant bacteria pose one of the major threats to human health worldwide. The issue is fundamental in the case of chronic wound treatment. One of the latest trends to overcome the problem is the search for new antibacterial agents based on silver. Thus, the aim of this research was to synthesize the silver-lactoferrin complex as a new generation of substances for the treatment of infected wounds. Moreover, one of the tasks was to investigate the formation mechanisms of the respective complexes and the influence of different synthesis conditions on the features of final product. The batch-sorption study was performed by applying the Langmuir and Freundlich isotherm models for the process description. Characterization of the complexes was carried out by spectroscopy, spectrometry, and separation techniques, as well as with electron microscopy. Additionally, the biological properties of the complex were evaluated, i.e., the antibacterial activity against selected bacteria and the impact on L929 cell-line viability. The results indicate the formation of a heterogeneous silver-lactoferrin complex that comprises silver nanoparticles. The complex has higher antibacterial strength than both native bovine lactoferrin and Ag+, while being comparable to silver toxicity.


Subject(s)
Metal Nanoparticles , Silver , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Bacteria , Chemical Phenomena , Humans , Lactoferrin/pharmacology , Metal Nanoparticles/chemistry , Microbial Sensitivity Tests , Silver/chemistry , Silver/pharmacology
4.
Int J Mol Sci ; 22(9)2021 Apr 29.
Article in English | MEDLINE | ID: mdl-33946710

ABSTRACT

Considering the advent of antibiotic resistance, the study of bacterial metabolic behavior stimulated by novel antimicrobial agents becomes a relevant tool to elucidate involved adaptive pathways. Profiling of volatile metabolites was performed to monitor alterations of bacterial metabolism induced by biosynthesized silver nanoparticles (bio-AgNPs). Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae and Proteus mirabilis were isolated from pressure ulcers, and their cultures were prepared in the presence/absence of bio-AgNPs at 12.5, 25 and 50 µg mL-1. Headspace solid phase microextraction associated to gas chromatography-mass spectrometry was the employed analytical platform. At the lower concentration level, the agent promoted positive modulation of products of fermentation routes and bioactive volatiles, indicating an attempt of bacteria to adapt to an ongoing suppression of cellular respiration. Augmented response of aldehydes and other possible products of lipid oxidative cleavage was noticed for increasing levels of bio-AgNPs. The greatest concentration of agent caused a reduction of 44 to 80% in the variety of compounds found in the control samples. Pathway analysis indicated overall inhibition of amino acids and fatty acids routes. The present assessment may provide a deeper understanding of molecular mechanisms of bio-AgNPs and how the metabolic response of bacteria is untangled.


Subject(s)
Bacteria/drug effects , Bacteria/metabolism , Metal Nanoparticles/therapeutic use , Pressure Ulcer/drug therapy , Pressure Ulcer/microbiology , Silver/therapeutic use , Volatile Organic Compounds/metabolism , Anti-Bacterial Agents/therapeutic use , Bacteria/isolation & purification , Drug Resistance, Bacterial , Enterococcus faecalis/drug effects , Enterococcus faecalis/isolation & purification , Enterococcus faecalis/metabolism , Escherichia coli/drug effects , Escherichia coli/isolation & purification , Escherichia coli/metabolism , Humans , In Vitro Techniques , Klebsiella pneumoniae/drug effects , Klebsiella pneumoniae/isolation & purification , Klebsiella pneumoniae/metabolism , Metabolic Networks and Pathways/drug effects , Metabolome/drug effects , Metabolomics , Microbial Sensitivity Tests , Proteus mirabilis/drug effects , Proteus mirabilis/isolation & purification , Proteus mirabilis/metabolism , Volatile Organic Compounds/chemistry , Volatile Organic Compounds/classification
5.
Molecules ; 26(6)2021 Mar 22.
Article in English | MEDLINE | ID: mdl-33810121

ABSTRACT

Volatile organic compounds (VOCs) have been assessed in breath samples as possible indicators of diseases. The present study aimed to quantify 29 VOCs (previously reported as potential biomarkers of lung diseases) in breath samples collected from controls and individuals with lung cancer, chronic obstructive pulmonary disease and asthma. Besides that, global VOC profiles were investigated. A needle trap device (NTD) was used as pre-concentration technique, associated to gas chromatography-mass spectrometry (GC-MS) analysis. Univariate and multivariate approaches were applied to assess VOC distributions according to the studied diseases. Limits of quantitation ranged from 0.003 to 6.21 ppbv and calculated relative standard deviations did not exceed 10%. At least 15 of the quantified targets presented themselves as discriminating features. A random forest (RF) method was performed in order to classify enrolled conditions according to VOCs' latent patterns, considering VOCs responses in global profiles. The developed model was based on 12 discriminating features and provided overall balanced accuracy of 85.7%. Ultimately, multinomial logistic regression (MLR) analysis was conducted using the concentration of the nine most discriminative targets (2-propanol, 3-methylpentane, (E)-ocimene, limonene, m-cymene, benzonitrile, undecane, terpineol, phenol) as input and provided an average overall accuracy of 95.5% for multiclass prediction.


Subject(s)
Adenocarcinoma of Lung/metabolism , Asthma/metabolism , Gas Chromatography-Mass Spectrometry , Lung Neoplasms/metabolism , Pulmonary Disease, Chronic Obstructive/metabolism , Volatile Organic Compounds/metabolism , Adult , Breath Tests , Female , Humans , Male
6.
Clin Oral Investig ; 25(7): 4415-4430, 2021 Jul.
Article in English | MEDLINE | ID: mdl-33387033

ABSTRACT

OBJECTIVES: Endogenous substances have been analyzed in biological samples in order to be related with metabolic dysfunctions and diseases. The study aimed to investigate profiles of volatile organic compounds (VOCs) from fresh and incubated saliva donated by healthy controls, individuals with oral tissue lesions and with oral cancer, in order to assess case-specific biomarkers of oxidative stress. MATERIALS AND METHODS: VOCs were pre-concentrated using headspace-solid phase microextraction and analyzed using gas chromatography-mass spectrometry. Then, VOCs positively modulated by incubation process were subtracted, yielding profiles with selected features. Principal component analysis and hierarchical cluster analysis were used to inspect data distribution, while univariate statistics was applied to indicate potential markers of oral cancer. Machine learning algorithm was implemented, aiming multiclass prediction. RESULTS: The removal of bacterial contribution to VOC profiles allowed the obtaining of more specific case-related patterns. Artificial neural network model included 9 most relevant compounds (1-octen-3-ol, hexanoic acid, E-2-octenal, heptanoic acid, octanoic acid, E-2-nonenal, nonanoic acid, 2,4-decadienal and 9-undecenoic acid). Model performance was assessed using 10-fold cross validation and receiver operating characteristic curves. Obtained overall accuracy was 90%. Oral cancer cases were predicted with 100% of sensitivity and specificity. CONCLUSIONS: The selected VOCs were ascribed to lipid oxidation mechanism and presented potential to differentiate oral cancer from other inflammatory conditions. CLINICAL RELEVANCE: These results highlight the importance of interpretation of saliva composition and the clinical value of salivary VOCs. Elucidated metabolic alterations have the potential to aid the early detection of oral cancer and the monitoring of oral lesions.


Subject(s)
Mouth Neoplasms , Volatile Organic Compounds , Gas Chromatography-Mass Spectrometry , Humans , Mouth Neoplasms/diagnosis , Saliva/chemistry , Solid Phase Microextraction , Volatile Organic Compounds/analysis
7.
J Clin Med ; 9(10)2020 Oct 01.
Article in English | MEDLINE | ID: mdl-33019642

ABSTRACT

Colorectal cancer (CRC) is the third most commonly diagnosed cancer in the world. In Europe, it is the second most common cause of cancer-related deaths. With the advent of metabolomics approaches, studies regarding the investigation of metabolite profiles related to CRC have been conducted, aiming to serve as a tool for early diagnosis. In order to provide further information about the current status of this field of research, 21 studies were systematically reviewed, regarding their main findings and analytical aspects. A special focus was given to the employment of matrices obtained non-invasively and the use of gas chromatography as the analytical platform. The relationship between the reported volatile and non-volatile biomarkers and CRC-related metabolic alterations was also explored, demonstrating that many of these metabolites are connected with biochemical pathways proven to be involved in carcinogenesis. The most commonly reported CRC indicators were hydrocarbons, aldehydes, amino acids and short-chain fatty acids. These potential biomarkers can be associated with both human and bacterial pathways and the analysis based on such species has the potential to be applied in the clinical practice as a low-cost screening method.

8.
Crit Rev Anal Chem ; 50(6): 501-512, 2020.
Article in English | MEDLINE | ID: mdl-31514505

ABSTRACT

Simple tests using sniff analysis that have the ability of diagnosing and rapidly distinguishing between infections due to different bacteria are urgently required by medical community worldwide. Professionals interested in this topic wish for these tests to be simultaneously cheap, fast, easily applicable, non-invasive, robust, reliable, and sensitive. Current analytical instrumentation has already the ability for performing real time (minutes or a few dozens of minutes) analysis of volatile bacterial biomarkers (the VOCs emitted by bacteria). Although many articles are available, a review displaying an objective evaluation of the current status in the field is still needed. This review tries to present an overview regarding the bacterial biomarkers released from in vitro cultivation of various bacterial strains and also from different biological matrices investigated, over the last 10 years. We have described results of relevant studies, which used modern analytical techniques to evaluate specific biomarker profiles associated with bacterial infections. Our purpose was to present a comprehensive view of available possibilities for detection of emitted bacterial VOCs from different matrices. We intend that this review to be of general interest for both medical doctors and for all researchers preoccupied with bacterial infectious diseases and their rapid diagnosis using analytical instrumentation.


Subject(s)
Bacterial Infections/diagnosis , Volatile Organic Compounds/metabolism , Animals , Bacteria/metabolism , Bacterial Infections/urine , Biomarkers/metabolism , Biomarkers/urine , Feces/chemistry , Feces/microbiology , Humans , Volatile Organic Compounds/urine
9.
J Clin Med ; 8(11)2019 Nov 19.
Article in English | MEDLINE | ID: mdl-31752439

ABSTRACT

Escherichia coli and salivary Klebsiella oxytoca and Staphylococcus saccharolyticus were subjected to different concentrations of silver nanoparticles (AgNPs), namely: 12.5, 50, and 100 µg mL-1. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) spectra were acquired after specified periods: 0, 1, 4, and 12 h. For study of volatile metabolites, headspace solid-phase microextraction coupled to gas chromatography/mass spectrometry (HS-SPME-GC-MS) was employed-AgNPs were added to bacteria cultures and the headspace was analyzed immediately and after 12 h of incubation. Principal components analysis provided discrimination between clusters of protein profiles belonging to different strains. Canonical correlation, network analysis, and multiple linear regression approach revealed that dimethyl disulfide, dimethyl trisulfide, 2-heptanone, and dodecanal (related to the metabolism of sulfur-containing amino acids and fatty acids synthesis) are exemplary molecular indicators, whose response variation deeply correlated to the interaction with bacteria. Therefore, such species can serve as biomarkers of the agent's effectiveness. The present investigation pointed out that the used approaches can be useful in the monitoring of response to therapeutic treatment based on AgNPs. Furthermore, biochemical mechanisms enrolled in the bactericidal action of nanoparticles can be applied in the development of new agents with enhanced properties.

10.
Anal Biochem ; 585: 113407, 2019 11 15.
Article in English | MEDLINE | ID: mdl-31449777

ABSTRACT

Escherichia coli (E. coli) is a Gram-negative bacterium commonly found in the lower intestine of warm-blooded organisms, including humans. Although the majority of the strains are considerably harmless, some serotypes are pathogenic, frequently causing diarrhea and other illnesses outside the intestinal tract. The standard antidote against bacteria is the use of antibiotics. Depending on their type, the antibiotics have various mechanisms of action on bacteria. Moreover, in case of in-vitro cultivation of bacteria, the used growth media plays a crucial role, since it influences bacterial inhibition as well. In the present study, we emphasize the importance of cultivability in bacterial inhibition under the treatment with five different antibiotics belonging to different classes. Consequently, E. coli was cultivated in three different growth media: trypcase soy broth (TSB), Mueller Hinton (MH), and minimal salts (M9) enriched with glucose, respectively. MALDI-TOF MS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry) analyses, that were used for fast characterization of changes that occur in ribosomal protein profiles, revealed differentiation and similarities between investigated cases, while flow cytometry (FCM) tests better explained the given changes that occurred in the analyzed samples after 3, 24 and 48 h of experimental campaign.


Subject(s)
Anti-Bacterial Agents/metabolism , Culture Media/chemistry , Culture Media/metabolism , Escherichia coli/drug effects , Escherichia coli/growth & development , Bacterial Proteins/analysis , Flow Cytometry , Glucose/chemistry , Ribosomal Proteins/analysis , Ribosomal Proteins/metabolism , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Time Factors
11.
Molecules ; 24(16)2019 Aug 16.
Article in English | MEDLINE | ID: mdl-31426356

ABSTRACT

Halitosis and submandibular abscesses are examples of mouth-related diseases with the possible bacterial origin. Salivary volatile organic compounds (VOCs) are potential biomarkers of them, once they can be addressed as metabolites of bacterial activity. Healthy patients (n = 15), subjects with submandibular abscesses located in fascial deep space (n = 10), and subjects with halitosis (n = 5) were enrolled in the study. Saliva samples were subjected to headspace solid-phase microextraction (HS-SPME) and gas chromatography coupled to mass spectrometry (GC/MS) analysis. A total number of 164 VOCs was detected by the developed methodology, 23 specific for halitosis and 41 for abscess. Halitosis' profiles were characterized by a larger number of sulfur compounds, while for abscess they had a higher variety of alcohols, aldehydes, and hydrocarbons-biomarkers of inflammatory processes. Principal components analysis allowed visualization of clusters formed according to the evaluated conditions. Kruskal-Wallis test indicated that 39 VOCs presented differentiated responses between the studied groups, with statistical relevance (p < 0.05). Random forest was applied, and a prediction model based on eight VOCs (2-butanone, methyl thioacetate, 2-methylbutanoic acid, S-methyl pentanethioate, dimethyl tetrasulfide, indolizine, pentadecane, and octadecanal) provided 100% of sensitivity, 82% of specificity, and 91% of balanced accuracy, indicating the specific presence of submandibular abscess.


Subject(s)
Abscess/diagnosis , Alcohols/isolation & purification , Aldehydes/isolation & purification , Halitosis/diagnosis , Hydrocarbons/isolation & purification , Sulfur Compounds/isolation & purification , Abscess/metabolism , Abscess/pathology , Adult , Aged , Alcohols/classification , Aldehydes/classification , Biomarkers/analysis , Case-Control Studies , Dentate Gyrus/metabolism , Dentate Gyrus/pathology , Diagnosis, Differential , Female , Gas Chromatography-Mass Spectrometry , Halitosis/metabolism , Halitosis/pathology , Humans , Hydrocarbons/classification , Male , Mandible/metabolism , Mandible/pathology , Middle Aged , Principal Component Analysis , Saliva/chemistry , Sensitivity and Specificity , Solid Phase Microextraction/methods , Sulfur Compounds/classification , Volatile Organic Compounds
12.
Anal Biochem ; 578: 36-44, 2019 08 01.
Article in English | MEDLINE | ID: mdl-31085164

ABSTRACT

Considering the shortcomings related to antibiotics usage, the introduction of other bacteriostatic and bactericidal agents that present synergetic effects or standalone properties is urgently needed. AgNO3 is an important bactericidal agent, which imparts various functions on bacteria dependent on its concentration. Therefore, an understanding of its mechanisms of action in infinitesimal concentrations plays an important role which can ultimately lead to AgNO3 involvement in the pharmaceutical industry. The monitoring of VOC (volatile organic compound) profiles emitted by bacteria is a simple method to assess changes occurring in bacterial metabolism. In this study, VOCs of Hafnia alvei, Pseudomonas luteola and Staphylococcus warneri cultures were analyzed both in the absence and in the presence of three concentrations of AgNO3. Headspace solid-phase microextraction gas chromatography/mass spectrometry (HS-SPME-GC/MS) was employed for extraction and analysis. After supplementation with AgNO3, changes in the emitted fingerprints were investigated. Odorants associated with mouth-related and systemic diseases, like dimethyl trisulfide, indole (halitosis) and 2-hexanone (celiac disease), were also affected by addition of AgNO3. Statistical tests proved discrimination between obtained profiles with more that 90% variability. Moreover, physiological states of bacteria after dosage with various concentration of stressing agent were investigated and explained by the mechanisms of action.


Subject(s)
Hafnia alvei/drug effects , Pseudomonas/drug effects , Saliva/microbiology , Silver Nitrate/pharmacology , Staphylococcus/drug effects , Volatile Organic Compounds/metabolism , Humans
13.
Electrophoresis ; 38(16): 2081-2088, 2017 08.
Article in English | MEDLINE | ID: mdl-28429817

ABSTRACT

The aim of the study was determination of bacterial viability in saliva samples and finding a correlation between microbiological and volatile profiles of saliva depending on incubation time. Bacteria colonizing healthy oral cavities were also identified. Twelve healthy adults donated unstimulated saliva samples. Flow cytometry, optical density measurements and colony-forming unit (CFU) counting method were employed for analyses of native and inoculated saliva after 0, 1, 2, 24, and 48 h of incubation. Volatile profiles were acquired using headspace-solid phase microextraction-gas chromatography/mass spectrometry (HS-SPME-GC/MS). Oral bacteria were the most viable within 2 h after collection of saliva. Extension of incubation time to 48 h caused considerable decrease in live bacteria counts and sharp increase in dead bacteria counts. The most prevalent strain was Sphingomonas paucimobilis (26.67%). The number of volatiles raised from 5 to 27 with incubation time and most of them were putrefaction products, such as methanethiol, indole and pyrrole. HS-SPME-GC/MS method is insufficient for volatile profiling of "fresh" saliva and should be directed rather to investigation of bacterial metabolites.


Subject(s)
Bacteria/isolation & purification , Microbial Viability , Saliva/microbiology , Volatile Organic Compounds/analysis , Adult , Cell Culture Techniques , Flow Cytometry , Gas Chromatography-Mass Spectrometry , Humans , Metabolome , Solid Phase Microextraction
14.
PLoS One ; 12(3): e0174521, 2017.
Article in English | MEDLINE | ID: mdl-28362838

ABSTRACT

The current work deals with the phenomenon of silver cations uptake by two kinds of bacteria isolated from dairy products. The mechanism of sorption of silver cations by Lactococcus lactis and Lactobacillus casei bacteria was investigated. Inductively coupled plasma-mass spectrometry (ICP-MS) was used for determination of silver concentration sorbed by bacteria. Analysis of charge distribution was conducted by diffraction light scattering method. Changes in the ultrastructure of Lactococcus lactis and Lactobacillus casei cells after treatment with silver cations were investigated using transmission electron microscopy observation. Molecular spectroscopy methods, namely Fourier transform-infrared spectroscopy (FT-IR) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) were employed for description of the sorption mechanism. Moreover, an analysis of volatile organic compounds (VOCs) extracted from bacterial cells was performed.


Subject(s)
Cations/metabolism , Lacticaseibacillus casei/metabolism , Lactococcus lactis/metabolism , Silver/metabolism , Dairy Products/microbiology , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Spectroscopy, Fourier Transform Infrared
15.
Crit Rev Anal Chem ; 47(3): 251-266, 2017 May 04.
Article in English | MEDLINE | ID: mdl-27905825

ABSTRACT

Saliva is an aqueous fluid found in the oral cavity playing fundamental role in the preservation and maintenance of oral health. Apart from higher molecular-weight constituents, it contains volatile organic compounds (VOCs) that may origin from such sources such as blood or be a result of bacterial metabolism. They can also reach the oral fluid through dissolving of volatiles from breath and consumed foods. Some of them may serve as biomarkers of various diseases, such as dimethyl disulfide (halitosis), pyridine (periodontal disease) and trimethylamine (trimethylaminuria) or occupational exposure. The review shows the basic functions of saliva and its composition. Furthermore, this paper presents the methodologies employed for sampling, extraction and enrichment of salivary VOCs and reviews the literature dedicated to profiling and searching of biomarkers emanated from saliva. Complete tables of reported VOCs and potential biomarkers from this specimen are also included.


Subject(s)
Saliva/chemistry , Volatile Organic Compounds/analysis , Biomarkers/analysis , Body Fluids/chemistry , Humans , Mouth/chemistry
16.
J Am Chem Soc ; 138(25): 7899-909, 2016 06 29.
Article in English | MEDLINE | ID: mdl-27263865

ABSTRACT

The process of silver immobilization onto and/or into bovine lactoferrin (LTF), the physicochemical properties of bovine lactoferrin and obtained silver-lactoferrin complexes, as well as antibacterial activity of silver-lactoferrin complexes were investigated in this work. Kinetic study of the silver immobilization into lactoferrin was carried out using batch sorption techniques. Spectrometric (MALDI-TOF/TOF-MS, ICP-MS), spectroscopic (FTIR, SERS), electron microscopic (TEM) and electrophoretic (I-DE) techniques, as well as zeta potential measurements, were applied for characterization of LTF and binding nature of silver in Ag-LTF complexes. On the basis of the results of the kinetics study, it was established that the silver binding to LTF is a heterogeneous process involving two main stages: (i) internal diffusion and sorption onto external surface of lactoferrin globules; and (ii) internal diffusion and binding into lactoferrin globule structure. Spectroscopic techniques combined with TEM analysis confirmed the binding process. Molecular dynamics (MD) analysis was carried out in order to simulate the mechanism of the binding process, and locate potential binding sites, as well as complement the experimental findings. Quantum mechanics (QM) simulations were performed utilizing density functional theory (DFT) in order to support the reduction mechanism of silver ions to elemental silver. Antimicrobial activity of synthesized lactoferrin complexes against selected clinical bacteria was confirmed using flow cytometry and antibiograms.


Subject(s)
Anti-Infective Agents/chemistry , Lactoferrin/chemistry , Metal Nanoparticles/chemistry , Silver/chemistry , Animals , Aspartic Acid/chemistry , Bacteria/drug effects , Binding Sites , Cattle , Drug Design , Glutamic Acid/chemistry , Ions , Microbial Sensitivity Tests , Molecular Dynamics Simulation , Nanotechnology , Protein Binding , Pseudomonas aeruginosa/drug effects , Quantum Theory , Software , Surface Properties
17.
Clin Chim Acta ; 433: 150-6, 2014 Jun 10.
Article in English | MEDLINE | ID: mdl-24657184

ABSTRACT

BACKGROUND: Low concentration of plasma pyridoxal-5-phosphate (PLP) is associated with hyperhomocysteinemia and inflammation. Most methods for the measurement of plasma PLP require large specimen volume and involve the use of toxic reagents. METHODS: We have developed a HPLC method for the measurement of PLP and 4-pyridoxic acid (4-PA) in plasma, which requires small specimen volume. The samples are prepared without adding any toxic reagents. Furthermore, we have examined whether intake of vitamin B6 affects the concentration of plasma PLP and 4-PA. RESULTS: The coefficient of variation of the method was 6% and the recovery of the added vitamin in plasma was about 100%. The concentrations of plasma PLP and 4-PA in 168 healthy subjects were 40.6 (8.4-165.0) nmol/L, median and (range) and 17.5 (3.7-114.79) nmol/L, median and (range) respectively. In the multiple regression analyses, the concentration of plasma PLP was associated with the concentration of plasma 4-PA (p<0.0001), BMI, (p=0.02) and sex, (p=0.0008). The concentration of plasma 4-PA was associated with plasma PLP (p<0.0001), serum folate (p=0.004), smoking (p=0.03) and vitamin B6 intake (p=0.01). CONCLUSION: The present method is suitable for large clinical studies for the measurement of plasma PLP and 4-PA. Our findings demonstrate that plasma 4-PA, BMI and sex are the major determinants of plasma PLP in healthy individuals.


Subject(s)
Blood Chemical Analysis/methods , Chromatography, High Pressure Liquid/methods , Pyridoxal Phosphate/blood , Pyridoxic Acid/blood , Adolescent , Adult , Aged , Calibration , Female , Healthy Volunteers , Humans , Male , Middle Aged , Young Adult
18.
Exp Ther Med ; 5(2): 572-580, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23407653

ABSTRACT

The clinical presentation of asthma results from complex gene-gene and gene-environment interactions. The natural variability of the DNA sequence within the NR3C1 gene affects the activity of glucocorticoid receptors (GCRs). The NR3C1 gene is localized on chromosome 5q31-q32. The gene coding for the GCR comprises nine exons. The structural domains of the GCR determine the biological functions of the functional domains. The observed resistance to glucocorticosteroids and the normal metabolic profile of Tth111I single nucleotide polymorphism (SNP) carriers is due to the ER22/23EK polymorphism that is present in them. BclI polymorphism significantly affects the process of alternative NR3C1 gene splicing and within that mechanism increases the sensitivity to glucocorticoids (GCs). A total of 451 subjects were enrolled in the present study, including 235 qualified to the group of bronchial asthma patients. A group of 216 healthy participants with no history of asthma or atopic conditions was qualified for the study. Genotyping was accomplished using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR-high resolution melting (HRM) methods. No statistically significant differences were observed in the frequency of Tth111I, BclI and ER22/23EK polymorphisms of the NR3C1 gene when comparing mild, moderate and severe asthma vs. the control group. Investigative analyses demonstrated statistically significant correlations for alleles and genotypes of Tth111I polymorphism of the NR3C1 gene between healthy subjects and patients with severe asthma characterized by a control profile corresponding to an Asthma Control Test (ACT)™ score ≥20. It was established that only the Tth111I polymorphism of the NR3C1 gene plays an important role in the pathogenesis of chronic bronchitis leading to the development of asthma with both allergic and non-allergic etiology.

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