ABSTRACT
Recruitment Consultants use Executive Searches - headhunting - to fill key vacancies in client companies. They cultivate a thorough understanding of the ideal candidate profile and the client's culture/strategy, and undertake a focussed search to identify competent individuals. A well-informed Consultant - sensitive to the requirements of both the client and the candidates throughout - will ultimately introduce the correct candidate to the correct job, to the benefit of all concerned.
ABSTRACT
We have initiated work towards the construction of YAC clone contigs across a repeat sequence island region on the mouse X Chromosome (Chr). The repeat sequence island region-the 141 island-located at band A3 contains 50 copies of a localized long complex repeat unit (LCRU). We have isolated 87 YAC clones from the 141 island and have used a dual faceted approach towards the construction of contigs across the repeat sequence island. First, we have identified YAC clones originating from the same region of the island by the identification of commonly held LCRU restriction site variants. Second, we have constructed rare cutter restriction maps of each YAC clone. Taken together, we have been able to assemble one large contig of 2.8 Mb and a number of smaller contigs. In total, contigs covering 5Mb of the island region have been identified. The island region would appear to represent a major component of the A3 Giemsa dark band on the mouse X Chr.
Subject(s)
Repetitive Sequences, Nucleic Acid , X Chromosome/genetics , Animals , Base Sequence , Chromosomes, Artificial, Yeast/genetics , Cloning, Molecular , DNA Fingerprinting , DNA Primers/genetics , Female , In Situ Hybridization, Fluorescence , Mice , Molecular Sequence Data , Restriction MappingSubject(s)
Fishes/genetics , Genome , Animals , Biological Evolution , Genome, Human , Humans , Mammals/genetics , Models, Genetic , Repetitive Sequences, Nucleic Acid , Vertebrates/geneticsABSTRACT
We have constructed a new generation yeast artificial chromosome (YAC) library from female C57BL/10 mice in a recombination-deficient strain of Saccharomyces cerevisiae carrying a mutation in the RAD52 gene. The YAC library contains 41,568 clones with an average insert size of 240 kilobases, representing a greater than threefold coverage of the mouse genome. Currently, the library can be screened by polymerase chain reaction and we have isolated positive clones at a number of loci in the mouse genome. This rad52 library should enable a long-term assessment of the effect of one of the yeast recombination pathway genes on both, genome-wide YAC clone stability and the frequency of chimaeric clones.
